Harvard University

Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen

Acanthamoeba castellanii causes infectious blindness and resides in a key evolutionary outgroup to humans and fungi. Its divergent mitochondria are of outstanding interest due to their predicted aerobic and anaerobic functions with potential for drug targeting. However, a detaile...

Acanthamoeba ATP synthase structure reveals the TCA cycle is tethered to OXPHOS

Oxidative phosphorylation (OXPHOS) is a key metabolic process that couples redox energy to ATP production. While some core OXPHOS complex subunits are found across all domains of life, many have diverged or expanded across evolution—as seen in the protozoan pathogen Acanthamoeba...

Murmurations, Mestre–Nagao sums, and convolutional neural networks for elliptic curves

Abstract We apply one-dimensional convolutional neural networks to the Frobenius traces of elliptic curves over $$\mathbb {Q}$$ Q and evaluate and interpret their predictive capacity. In keeping with similar experiments by Kazalicki–Vlah, Bujanović–Kazalicki–Novak, and Pozdnyakov...

Big data and psychiatry: advances, constraints and future directions.

Early work in psychiatry research, often involving single sites, small samples, and limited variables, has shifted to contemporary research involving multiple sites, large samples, and many variables. Such research raises important questions, including concerns about data quality...

Conserved and divergent mitochondrial assemblies in kinetoplastid parasites

The mitochondrial proteomes of Leishmania tarentolae and Trypanosoma brucei contain ∼1,700 proteins, most of which lack homologs in higher eukaryotes. Here, we integrate complexome profiling and cryo-electron microscopy to define conserved and lineage-specific macromolecular asse...

Rectifying market externalities via AI policymaking

Abstract Traditional competitive markets do not account for negative externalities; indirect costs that some participants impose on others, such as the cost of over-appropriating a common-pool resource (which diminishes future stock, and thus harvest, for everyone). Quantifying a...

Single-cell profiling and genetic regulation of alternative polyadenylation in the human brain

Abstract Alternative polyadenylation (APA) has a central role in regulation of the fate of mRNA and exhibits high variability in the brain and in neurons. However, brain APA regulation has not been mapped at cell type resolution, and the genetic control of APA in brain cell types...

Mixed updating in structured populations

Evolutionary graph theory (EGT) studies the effect of population structure on evolutionary dynamics. The vertices of the graph represent the N individuals. The edges denote interactions for competitive replacement. Two standard update rules are death-Birth (dB) and Birth-death (B...

The global cost of schizophrenia: a health economic modelling study

Abstract Background Schizophrenia is a chronic psychiatric disorder that leads to substantial disability, impaired quality of life, and reduced life expectancy. In addition to its profound impact on affected individuals, the disorder places a considerable financial burden on heal...

Assessing Individual Differences in Adult Theory of Mind Through Mental State Language: Human and Artificial Intelligence Approaches

Theory of mind is central to human social behavior, yet empirical studies rely largely on biased measures with limited ecological validity. We propose and evaluate the use of mental state language as a spontaneous indicator of theory of mind expressed in social interaction. Among...

Comprehensive profiling of brain dynamics during anesthesia across phylogeny

Abstract The behavioral effects of anesthetics are highly conserved across species, hinting at shared and fundamental underlying mechanisms. Here we compile a dataset of multiscale neural activity during wakefulness and anesthesia, encompassing human, macaque, marmoset, mouse, ze...

Melanoma Prognostication Using AI ‐Guided Histopathology

Accurate diagnosis and risk stratification are central for optimal management of patients with melanoma. Current American Joint Committee on Cancer (AJCC) staging systems inadequately stratify patients with clinically meaningful metastatic potential. Manual histopathologic interp...

Experimental model of ventilator-induced lung injury induces proteomic signatures of neurodegeneration in the hippocampus

Abstract Background Mechanical ventilation is associated with acute and long-term cognitive dysfunction, yet the molecular pathways linking ventilator-induced lung injury (VILI) to brain injury remain poorly characterized. We previously demonstrated that peripheral IL-6 signaling...

Coblation Versus Microdebrider in Pediatric Intracapsular Tonsillotomy: A Comparison of Efficiency, Bleeding, and Revision Surgery

OBJECTIVE: Intracapsular tonsillectomy or "tonsillotomy" is an increasingly popular alternative to extracapsular tonsillectomy due to decreased bleeding risk and pain. Coblation and microdebrider are most frequently used, but studies comparing them are limited. We aimed to compar...

Revealing the Proton Slingshot Mechanism in Solid Acid Electrolytes through Machine-Learning Molecular Dynamics

Abstract In solid acid electrolytes CsH2PO4 and CsHSO4, the origin of fast proton conduction has long been debated, attributed either to local proton hopping or to polyanion rotation. However, the precise role of polyanion rotation and its interplay with proton hopping remains un...

Preconception, Prenatal and Postnatal PM2.5 Exposure and Neurodevelopmental Disorder Risk among Medicaid Recipients

Abstract Evidence linking ambient fine particulate matter (PM2.5) exposure to childhood neurodevelopmental disorders (NDDs) remains mixed. We conducted a cohort study (2001–2013) of 1,547,244 Medicaid-enrolled mother-child pairs to assess associations between zip-code–level PM2.5...

Comparative Renal Effectiveness of GLP-1RA and SGLT2i in US Veterans with Type 2 Diabetes and CKD

Objective: To address limited comparative evidence in older, clinically complex patients with type 2 diabetes and moderate-to-advanced chronic kidney disease (CKD), we compared nephroprotective outcomes after GLP-1RA versus SGLT2i initiation among US Veterans aged ≥65 years with...

Can We Diagnose Early Melanoma by Genomics?

Melanoma incidence has increased approximately 6-fold over the past 40 years, driven primarily by melanoma in situ and thin invasive melanomas, which are among the most subjective lesions to diagnose on histopathology. This review examines whether genomics clarifies the "gray zon...

Conversational themes and interaction patterns in peer support for adolescents and young people living with HIV: scoping review protocol

Peer support for adolescents and young people living with HIV (AYPLHIV) has demonstrated effectiveness in improving clinical and psychosocial outcomes. However, the specific conversational content and interaction patterns that underpin successful peer support remain poorly charac...

Accumulating safety data for remote ischemic conditioning in acute pediatric neurological conditions

Acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) is the most common infection-triggered encephalopathy in East Asian children and one for which existing neuroprotective strategies are limited. Despite the use of therapeutic hypothermia (TH) or targete...