Columbia University

The clinical and molecular spectrum of AGO2-associated Lessel-Kreienkamp neurodevelopmental syndrome

Abstract Background Pathogenic variants in AGO2 ,encoding a central component of the RNA-induced silencing complex (RISC), cause the neurodevelopmental disorder Lessel-Kreienkamp syndrome (LESKRES). The variant spectrum and associated molecular mechanisms underlying phenotypic va...

Feasibility and acceptability of adding peer support to a school-based nurse-led smoking cessation intervention among male adolescents in Pakistan: a pilot cluster-randomized trial

Reducing adolescent smoking is a global health priority. School-based, nurse-led interventions show promise, yet most studies have been conducted in Western and other high-income countries. This study assessed the feasibility and acceptability of a school-based, nurse-led smoking...

Benchmarking generative AI tools for literature retrieval and summarization in genomic variant interpretation

Abstract Background Generative AI is increasingly used to extract structured information across domains, but its reliability in academic and clinical research, where precision and accuracy are essential, remains largely unexplored. This study evaluates the ability of Large Langua...

Dual-oxygen pancreatic cancer organoids recapitulate basal–classical heterogeneity validated by spatial transcriptomics

Pancreatic ductal adenocarcinoma (PDAC) comprises heterogeneous classical and basal-like subtypes. Although pancreatic cancer organoids (PCOs) are widely used, normoxic cultures preferentially maintain the classical subtype, limiting their ability to represent intrinsic intratumo...

Transfer Learning of CATE with Kernel Ridge Regression

The proliferation of data has sparked significant interest in leveraging findings from one study to estimate treatment effects in a different target population without direct outcome observations. However, the transfer learning process is frequently hindered by substantial covari...

Dysregulated adult hippocampal neurogenesis in major depressive disorders

Major depressive disorder (MDD) is associated with reduced hippocampal volume, altered connectivity and negative memory bias, suggesting disrupted hippocampal plasticity. Dysregulated adult hippocampal neurogenesis is a potential contributor, but its relevance in humans and role...

Emergence of novel SARS-CoV-2 variants keeps slowing down

Abstract Background Although COVID-19 pandemic has no longer been classified as an international public health emergency of concern after May 2023, multiple variants with different characteristics keep emerging. Given the complex spatial-temporal characteristics of epidemics, res...

Bayesian inference of lineage trees by joint analysis of single-cell multimodal lineage-tracing data with BiLinT

Abstract The advent of single-cell lineage-tracing technologies has enabled simultaneous measurement of gene expression and lineage barcodes. However, integrating these modalities for high-resolution lineage reconstruction has remained challenging due to limitations of methods th...

Bidirectional AllostericLigand Regulation in a CentralGlycolytic Enzyme

Abstract Allosteric regulation enables fine-tuned control of enzyme activity in response to cellular signals, yet its molecular basis often remains unclear. Phosphofructokinase-1 (PFK), the highly conserved, rate-limiting glycolytic enzyme, is a paradigm for Monod–Wyman–Changeux...

Time-dependent divergence in infection risks among patients with multiple sclerosis treated with fumarates versus anti-CD20 monoclonal antibodies

Background: People with multiple sclerosis (pwMS) treated with anti-CD20 monoclonal antibodies have an elevated infection risk, yet long-term comparative data with oral fumarates are limited. Objective: To compare infection incidence, healthcare resource utilization, and relapse...

Optimizing culture-free approaches to recover high-quality Mycobacterium tuberculosis genomic variation

Background. Mycobacterium tuberculosis ( Mtb ) genomic epidemiology often relies on culturing patient sputum, a time- and labour-intensive process. Hybrid capture approaches have been successfully used to enrich Mtb DNA from complex clinical samples, yet the accuracy of variant i...

Retail Electricity Costs and Emissions Incentives Are Misaligned for Commercial and Industrial Power Consumers

Abstract Electrification is contributing to substantial growth in U.S. commercial and industrial loads, but the cost and Scope 2 carbon emission implications of this load growth are opaque for both power consumers and utilities. We present a comprehensive spatiotemporal data set...

Altered thalamo–prefrontal synchrony dynamics during spatial working memory task performance in a SETD1A loss-of-function mouse model of schizophrenia predisposition

ABSTRACT Schizophrenia is associated with profound working memory deficits, for which there are no approved treatments. Rare heterozygous null mutations in SETD1A , a gene encoding a key epigenetic regulatory protein, have been definitively linked to increased risk for schizophre...

Molecular Designof Flotation Collectors: Impact ofan Allyl Group on Thionocarbamate Adsorption

Abstract Thionocarbamates, particularly N-ethyl O-isopropyl thionocarbamate (IPETC), are widely used as collectors in froth flotation. The recently developed N-allyl O-isobutyl thionocarbamate (AIBTC) shows broader applicability and enhanced recovery for coarse and fine particles...

Therapeutic Reversalof PH-HFpEF via Lung SpheroidCell Exosomes

Abstract Pulmonary hypertension–associated heart failure with preserved ejection fraction (PH-HFpEF) is a prevalent and lethal cardiopulmonary syndrome without effective disease-modifying therapy. We investigated the therapeutic potential of lung spheroid cell–derived exosomes (L...

Challenges to treatment preparedness among lung cancer patients from a healthcare provider perspective: a mixed-method study

We aim to (1) explore the main barriers to treatment faced by lung cancer patients, from a provider’s perspective, and (2) the existing support systems in addition to recommended mitigation strategies to enhance the preparedness process. Our study invol ves 50 surveys and 10 inte...

Less air pollution previously related to clean air and climate policies is linked to improved cognitive scores over time in NYC children

Prenatal and postnatal exposure to air pollution has been associated in multiple epidemiological studies with adverse neurodevelopmental outcomes in children, including in New York City cohorts. Studies are lacking on changes in associations between air quality and neurodevelopme...

Highly attenuated dendritic propagation of isolated synaptic potentials in vivo

The integration of synaptic inputs is a fundamental function of neurons. In the traditional model, excitatory inputs are summed at the soma to generate action potentials. However, how synaptic inputs are integrated by dendrites in vivo remains poorly explored. We used intravital...

Photonic-crystal hydraulic manometry quantifies epithelial basal compression for rapid, label-free functional screening

Polarized epithelia integrate barrier sealing, vectorial ion-water transport, and cytoskeletal mechanics, yet scalable assays that report this coupled functional state in real time remain limited. Here, we introduce photonic-crystal hydraulic manometry (PCHM), which quantifies ou...

Optimizing Inventory Placement for a Downstream Online Matching Problem

Problem definition: We study the inventory placement problem of splitting [Formula: see text] units of a single item across warehouses in advance of a downstream online matching problem that represents the dynamic fulfillment decisions of an e-commerce retailer. This is a challen...