One Health genomic surveillance identifies potential genetic links of antimicrobial-resistant foodborne pathogens across sectors
Abstract
Foodborne pathogens and antimicrobial resistance are major global health concerns. The integration and analysis of genomic surveillance data across human, animal, and environmental sectors remain a critical challenge in the study of foodborne bacteria. Here we investigate potential cross-sector genetic links between antimicrobial-resistant isolates from food (mainly chicken meat), animals (mainly cecal and rectal samples), and humans (clinical cases) by comparing whole-genome sequences of non-typhoidal Salmonella (n = 1,613), Campylobacter (n = 682), and Enterococcus (n = 72) collected from these sectors across Japan. For Salmonella, we identify closely related human- and food-derived S. Blockley isolates carrying blaCTX-M-15, with pairwise SNP distances of 6–10 SNPs, as well as S. Agona isolates carrying fosA7.2, with pairwise SNP distances of 13–15 SNPs, suggesting that resistant Salmonella strains are shared between human and food sectors. For Campylobacter, we identified closely related human- and food-derived ST-22 isolates carrying a tetracycline resistance gene and a fluoroquinolone resistance-conferring gyrA mutation, with a pairwise SNP distance of 4 SNPs. These findings highlight potential genetic links of antimicrobial-resistant foodborne bacteria between food and humans. In this study, the authors use nationwide genomic data collected across sectors in Japan to identify closely related antimicrobial-resistant Salmonella and Campylobacter isolates in food and humans, highlighting the value of integrated One Health surveillance.
// Source
Authors: Norikazu Kitamura, Hiroto Shinomiya, Michiko Kawanishi, Noriko Konishi, Kenji Ohya, Haruyoshi Tomita, Yukiko Asano, Chizuko Kimura, Hideto Sekiguchi, Manao Ozawa, Kanako Ishihara, Jun Kurushima, Koichi Tanimoto, Takahiro Nomura, Yusuke Hashimoto, Shintaro Hirai, Yuka Fukuguchi, 紀子 青木, Yuka Otsuka, Yamagami Takeshi, Yohei Takahashi, Yukina Hashimoto, Shihomi Osanai, Takashi Iwama, Chizuko Sato, Tomoko Yazaki, Satoko Yamaya, Mai Shiina, Yoko Kimura, Natsuki Ueda, Naoshi Ando, Miyuki Suzuki, Tomoka Masaoka, Ichiro Furukawa, Chieko Goto, Mitsumasa Koizumi, Yuko Mizuno Matsumoto, Keita Yanagimoto, Keiko Kimata, Emi Maenishi, Junko Isobe, Kaho Ikeda, 治樹 石森, 暁洋 永田, Nobushige Sakai, Koji Yokoyama, Shiho Tajima, Satomi Iwasaki, Miho Toho, Miyuki Saeki, Yui Tsukiyama, Shunya Nishijima, Yuki Wakabayashi, Junko Sakata, 岩崎直昭, Katsunori Nakano, Takahito Tano, Hiromi FUKUDA, Etsuko Saito, Kenichi Ogita, Yuta Kawakami, Hiroki Hayashi, Ryoji Nomura, Hisahiro Kawai, Tomohiro Kajihara, Kazumi Ikeda, Hideaki Kariya, 末永朱美, Nobuyo Ikeda, Ayaka Chigami, 蔵田和正, Yukie Ohara, Kaori Sato, Miwako Yamamoto, Chiemi Fukuda, Kazumi Seki, Yoko Iwashita, Kyoko Meguro, Etsuko Nakamura, Kanako Ueno, Chinami Hakataya, Hironobu Ooba, Michiko Fujisaki, Tetsuo Asai, Haruo Watanabe, Yo Sugawara, Koji Yahara, Motoyuki Sugai
Institutions: Gunma University, National Institute of Infectious Diseases, Tokyo University of Agriculture and Technology, Toyama Institute of Health, Gifu University, Kanagawa Prefectural Institute of Public Health, National Institute of Health Sciences, Japan Institute for Health Security, Ehime Prefectural Institute of Public Health and Environmental Science, Ministry of Agriculture, Forestry and Fisheries, National Veterinary Assay Laboratory, Tokyo Metropolitan Institute of Public Health, Miyagi Prefectural Institute of Public Health and Environment, Chiba Prefectural Institute of Public Health, Yokohama City Institute of Public Health, Yamanashi Institute for Public Health and Environment, Okayama Prefectural Institute for Environmental Science and Public Health, Fukui Prefectural Institute of Public Health and Environmental Science, Nara Prefectural Institute of Public Health Research Center, Osaka Institute of Public Health, Sakai City Institute of Public Health, Hyogo Prefectural Institute of Environmental Sciences, Shimane Prefectural Institute of Public Health and Environmental Sciences, Hiroshima City Institute of Public Health, Kagawa Prefectural Research Institute for Environmental Sciences and Public Health, Kitakyushu City Institute of Health and Environmental Sciences