A pair of chitosan receptors confer broad-spectrum fungal resistance
Abstract
Chitosan, a major fungal cell wall component, activates plant immunity, yet the underlying perception mechanism remains unknown. In this study, we identified OsFID1, an apoplast-localized DUF26 domain protein, as a chitosan-binding receptor essential for chitosan-triggered immunity. OsFID1 forms a receptor complex with OsFID2, a plasma membrane–localized DUF26 receptor-like kinase, to mediate chitosan sensing and immune activation, requiring OsFID2 phosphorylation for function. The OsFID1-OsFID2 complex confers broad-spectrum resistance to fungal pathogens. OsFID1 homologs are conserved in land plants, which show chitosan-binding activity in both monocots and dicots. Ectopic expression of OsFID1 in wheat and maize enhanced fungal resistance without growth penalty. These findings uncover a conserved receptor complex for direct chitosan sensing, advancing our understanding of plant-fungus interactions and providing a potential strategy for engineering durable fungal resistance crops.
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Authors: Keyi Huang, Jingni Wu, Yu Cao, Yingying Cao, Zhongji Pu, Aoshen Xu, Wenzhao Wang, Xue Zhou, Ming Li, Min Zhang, Yuhua Wang, Jinbiao Ma, Yulin Du, Qin Wang, Yingqiao Wan, Shangyu Gao, Dacheng Wang, Guangzheng Sun, Qin Gu, Huan Chen, Guosheng Xiong, Heng Yin, Yiwen Deng, Wensheng Zhao, Zhengguang Zhang, Shengben Li, Xiquan Gao, Sun Tae Kim, Gang Li, Baolei Jia, Jijie Chai, Kenichi Tsuda, Zheng Qing Fu, Yiming Wang
Institutions: Shanghai Jiao Tong University, University of South Carolina, Chinese Academy of Sciences, Zhejiang University, Westlake University, China Agricultural University, Nanjing Agricultural University, Northwest A&F University, Dalian Institute of Chemical Physics, Pusan National University, Anhui Agricultural University, Huazhong Agricultural University, Center for Excellence in Molecular Plant Sciences