Huazhong Agricultural University

A Poly(lactic Acid)‐Porphyrin‐Cobalt Vaccine Adjuvant System Elicits Protein‐ or Peptide‐Specific Humoral and Cellular Immunity

ABSTRACT There is growing interest in vaccine adjuvant systems that are scalable, adaptable, and capable of stimulating humoral and cellular immune responses with recombinant protein or synthetic peptide immunogens. In this study, we introduced a “plug‐and‐play” nanoadjuvant syst...

Synthesis of Bioactive Fluorinated 2-Heterocyclic Pyrrolidines via Copper(I)-Catalyzed Asymmetric 1,3-Dipolar Cycloaddition of Azomethine Ylides

Abstract A copper-catalyzed asymmetric 1,3-dipolar cycloaddition of α-N-(heteroaryl-methyl)imines with gem-difluoroalkenes is reported. Using a Cu(I)/Segphos complex, this protocol provides diverse chiral fluorinated 2-heterocyclic pyrrolidines in high yields (up to 99%) with exc...

A reproducible and efficient inoculation system for rice false smut based on conidia-germinated hyphae

Abstract Rice false smut, caused by the ascomycete fungus Ustilaginoidea virens , is one of the most severe diseases of rice. Conventional artificial inoculation methods for rice false smut rely mainly on conidia–hyphae mixtures (CH) as inocula, which frequently result in poor re...

A GRX-SOD module maintains superoxide homeostasis and meristem development in maize

Abstract The continuous initiation of plant aerial organs relies on the maintenance of dynamic stem cell homeostasis within the shoot apical meristem (SAM). Although reactive oxygen species (ROS) have emerged as crucial signals determining cell fate, how plants precisely maintain...

Heart failure may promote breast cancer progression through a Col10a1-associated Tgf-β/Smad-EMT pathway

Heart failure (HF) is clinically associated with aggravated breast cancer (BC) progression; however, the integrative genomic mechanisms underlying this cardio-oncological crosstalk remain poorly understood. We conducted integrative bioinformatics analyses combining weighted gene...

Complete mitogenome of Plagiognathops microlepis and its phylogenetic position within the subfamily Xenocyprinae (Cypriniformes: Cyprinidae)

The complete mitochondrial genome of Plagiognathops microlepis was sequenced using Illumina NovaSeq and PacBio Sequel II platforms.The circular mitogenome is 16,632 bp in length and contains 37 genes: 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (...

OccPepSeg-YOLO for Instance Segmentation of Occluded Peppers in Field Images

Agricultural operations such as pepper harvesting, fruit counting, and field phenotyping rely on accurate visual recognition and instance segmentation algorithms. However, pepper fruits in complex field environments often exhibit slender and curved shapes, partial occlusion, ambi...

Integrated physiological, transcriptomic and metabolomic analyses characterize treatment-associated autumn shoot self-pruning and maturation in Wogan mandarin

Autumn shoot maturation is important for citrus canopy management and subsequent reproductive development. We compared five foliar treatments but interpret their effects as treatment-specific because the salts were not equimolar and KH₂PO₄ supplied both K and P. Water (CK), 0.3%...

Beyond the factor-driven paradigm: Spatiotemporal dynamics and system coupling of agricultural transition in Henan Province, China

The core of agricultural transition lies in changes to the various components of the agricultural systems and their interactions. Drawing on the social-ecological-technological systems (SETS) framework, this study developed an “element coupling–external manifestation” analytical...

Iteratively DesignedPhospholipid-Targeted SupramolecularSonosensitizers for Precision Treatment of Pulmonary Infections

Abstract Fungal infections represent a significant global health threat, exacerbated by the scarcity of new therapeutic options, increasing drug resistance, and host toxicity. Sonodynamic therapy (SDT) offers a promising approach to overcoming antimicrobial resistance by enabling...

Threshold-window framework reveals management-sensitive controls of CH4 and N2O emissions in flooded rice

Rice paddies are major sources of methane (CH 4 ) and nitrous oxide (N 2 O), yet co-managing water and nitrogen (N) to limit both gases remains challenging because emissions arise from non-linear interactions among multiple drivers. We combined a two-year field experiment in subt...

Nutrient–Microbiota Co-Regulation of Protein Conversion in Black Soldier Fly Larvae: The Role of Alkali-Soluble Protein and Gut Microbial Communities

Insect protein farming provides sustainable advantages in land efficiency, emission reduction, and bioconversion. A major challenge is optimizing nutrient composition to achieve cost-effective protein production. This study investigates the co-regulatory mechanism between alkali-...

Biocontrol of tomato soilborne diseases by fungivorous nematode Aphelenchoides bicaudatus

<title>Abstract</title> Crop soil-borne diseases caused by pathogens, such as Fusarium spp. and root-knot nematodes, contribute to substantial yield losses worldwide. This study investigated the biocontrol potential of the fungivorous nematode, Aphelenchoides bicaudatus isolate A...

Thiram Disrupts Hepatic and Tibial Calcium Homeostasis via ER Stress‐Mediated IP3R1 / VDAC1 Hyperactivation

ABSTRACT Thiram is an agricultural fungicide known to disrupt cellular calcium homeostasis by inducing endoplasmic reticulum (ER) stress and mitochondrial calcium overload in hepatic and growth plate (GP) tissues. Current study investigated thiram‐induced alterations at mitochond...

Spinetoram‐based seed‐pelleting with synergistic mixtures enhances control of the diamondback moth

BACKGROUND: The diamondback moth, Plutella xylostella, is one of the most destructive pests of cruciferous crops worldwide. Its high fecundity, stress tolerance and seasonal migration often render traditional spray control ineffective. In this study, we propose an alternative met...

TPS10 contributes to linalool emission in rice in response to brown planthopper infestation

Abstract BACKGROUND Terpenoids, a prominent class of herbivore‐induced plant volatiles, play a crucial role in plant responses to insect attacks. However, the precise ecological functions of individual terpene synthases in rice defense mechanisms remain unclear. RESULTS The level...

A Label‐Free Bioluminescent scFv‐NLuc Fusion Probe Enabling a Smartphone‐Based Sensitive Immunoassay Platform Using Lomefloxacin as a Model Analyte

ABSTRACT Enzymes as labeling agents enhance lateral flow immunoassays (LFIA). However, their biological instability and reliance on labor‐intensive chemical conjugation hinder practical high‐throughput screening of food contaminants. To address this, we report a rapid, label‐free...

Land marketization, fiscal decentralization and carbon emissions

During China’s period of economic transition, its policy of fiscal decentralization (FD) has vigorously promoted China’s economic development, but it has brought about a mass of carbon emissions (CE) from its urban and industrial development. To solve environmental problems, the...

Genetic Determinants of Milk Production Phenotypes in Dairy Goats: A Review

Dairy goats sustain milk production across marginal environments worldwide, yet genetic improvement of milk yield and composition remains slower than in cattle, constrained by smaller reference populations and limited functional validation. This review consolidates current eviden...

Dual‐line Genome‐scale CRISPR Screening Enables Robust Target Gene Discovery

Pooled CRISPR screening enables genome-scale functional gene discovery by linking genetic perturbations to cellular phenotypes. However, its application in insects remains limited, as systematic differences often obscure the universal functional signals and compromise the reliabl...