University of Manchester

Structural basis of the gating mechanism of the large-conductance mechanosensitive channel from Escherichia coli

Abstract The mechanosensitive channel of large conductance (MscL) is a tension-gated, pore-forming protein that acts as a safety valve to protect bacteria from osmotic lysis. Escherichia coli MscL (EcMscL) was the first mechanosensitive channel discovered and subsequently served...

Strategic roadmap for delivery of clinical trials in rare childhood central nervous system (CNS) tumours: a multi-stakeholder consensus

Abstract Rare paediatric central nervous system (CNS) tumours comprise a biologically-heterogeneous group of low-incidence diseases that present significant challenges to both therapeutic development and clinical research, limiting the availability of high-quality evidence to gui...

Motivational Trajectories of Chinese Secondary School EFL Learners: Understanding Demotivation and Remotivation Through a Bioecological Lens

This study drew on bioecological systems theory to investigate factors influencing demotivation and remotivation among Chinese secondary school EFL learners. Adopting a sequential explanatory mixed-methods design, 129 Grade-12 students were asked to create motivational change gra...

Understanding bowel cancer screening behaviour and intention across British, Indian, and Indian-Immigrant populations: A qualitative cross-cultural comparison

Bowel cancer screening reduces mortality, yet uptake remains suboptimal across many populations. While disparities in participation are well documented, limited qualitative research has explored how cultural and healthcare contexts shape attitudes towards screening and preventive...

1.5 µm Emission Properties of Erbium‐Implanted Thin‐Film Lithium Niobate

ABSTRACT Lithium niobate on insulator (LNOI) has emerged as a promising platform for photonic integrated circuits due to its strong electro‐optic, piezoelectric and nonlinear properties. The incorporation of rare‐earth ions, particularly erbium (Er 3+ ), enables potential functio...

Paediatric therapeutic development workshop on osteosarcoma

The fourth Paediatric Therapeutic Development Workshop focused on osteosarcoma, the most common primary bone cancer in children and young adults. Current treatment of osteosarcoma comprises surgery and chemotherapy. Outcome has shown very little improvement over the last four dec...

Adaptive optics imaging in bilateral vitelliform maculopathy

This report presents the clinical, electrophysiological and multimodal imaging findings of an individual with bilateral vitelliform maculopathy. Adaptive optics imaging enables in vivo visualisation of retinal microstructure at cellular resolution, providing a detailed baseline f...

Tiny Droplets, Large Insights: Studying Peptide Aggregationwith Droplet-Based Microfluidics Hyphenated with Ion Mobility MassSpectrometry

Abstract Peptide and protein aggregation are hallmarks of numerous neurodegenerative diseases. However, the structural characterization of oligomeric intermediates remains challenging due to their low abundance, heterogenic nature, and complexity of coinciding and transient react...

Cross-cultural adaptation and validation of the Localised Scleroderma Quality of Life Instrument (LoSQI): an international study

Juvenile localised scleroderma (jLS) is a rare disease which impacts negatively on health-related quality of life (HRQoL). We aimed to undertake the cross-cultural adaptation and validation of a novel disease-specific HRQoL measure, the Localised Scleroderma Quality of Life Instr...

Acceptability of breast cancer risk assessment for women aged 30–49: Views of women from six under-served groups

Inequalities in breast screening uptake and cancer outcomes persist among under-served women. To reduce disparities, it is essential to understand women’s views when developing proactive early detection and prevention services. This study explored the acceptability of introducing...

A dynamic analysis of tenure status in Africa’s urban housing markets

There is a widespread narrative on the global housing crisis. To contribute to our collective understanding, this paper analyses housing outcomes in four urban centres in Ghana and Tanzania. We consider how outcomes change across five age cohorts differentiated by gender. We show...

VOC observations improve transferable ozone prediction using interpretable machine learning

Abstract Surface ozone variability is controlled by complex interactions among emissions, chemistry, and meteorology, yet the extent to which machine learning models capture chemically meaningful relationships remains uncertain. Here we apply an automated machine learning framewo...

Global Groundwater–CarbonCycling Shaped byTropical Basins and Extra-Tropical Sinks

Abstract Groundwater’s role in the global carbon cycle is poorly quantified compared to other carbon reservoirs, particularly on a global spatial scale. Here we show that the carbon stock of terrestrial groundwater is approximately equal to that stored in the modern atmosphere, a...

Retired doctors as users of patient-facing electronic health records: a mixed-methods survey in the UK and Spain

<title>Abstract</title> Background Online patient access to electronic records is expanding internationally but remains contested. Although clinicians’ concerns are well documented, less is known about how doctors’ views change when they themselves become patients. Retired doctor...

Optimal combination of composite likelihoods using approximate Bayesian computation with application to state-space models

Composite likelihood provides approximate inference when the full likelihood is intractable and sub-likelihood functions of marginal events can be evaluated relatively easily. It has been successfully applied for many complex models. However, its wider application is limited by t...

Hydro-demographic drivers of aquifer depletion and urban water insecurity in Borama, Somaliland: a mixed-methods assessment

Background In semi-arid, data-scarce regions, the convergence of climate change and rapid urbanization poses a severe threat to groundwater-dependent cities. This study evaluates the impact of hydro-climatic stressors and demographic growth on urban water security and aquifer dep...

Transforming biomedical uncertainty: the sociohistorical origins of myalgic encephalomyelitis (ME) and chronic fatigue syndrome (CFS)

Myalgic encephalomyelitis (ME) and chronic fatigue syndrome (CFS) are serious and disabling long-term conditions characterised by uncertainty surrounding their aetiology, diagnosis and treatment. People with ME/CFS struggle to be understood, taken seriously and supported with the...

Modelling dynamic blade loads on scaled tidal turbines due to wave-induced unsteady kinematics

Accurate prediction of wave-induced unsteady loads on tidal turbines is essential for reliable fatigue assessment and load mitigation design. These loads arise from the turbine rotating through a depth-varying flow field that oscillates at the wave frequency, requiring detailed m...

Expanding the BiocatalyticToolbox: A Singular P450Enzyme Efficiently Produces Diverse Pharmaceutical Metabolites

Abstract P450 BM3 is a natural fusion protein with a P450 domain fused to its necessary redox partner and capable of binding a range of fatty acids at high catalytic rates. The flexibility of the active site and catalytic efficiency have led to extensive research aimed at modifyi...

Use, trustworthiness and willingness to self-monitor health and personal wellness: a UK population survey

Abstract Background Public opinion about self-monitoring of health and personal wellness is important but largely unknown. This study investigated public attitudes towards self-monitoring of health and personal wellness. Methods Four thousand two hundred seventy UK adults selecte...