Peking University

Probing the Conformational Dynamics of VeCas9 Using High-Resolution Single-Molecule Junctions

Abstract Gene editing enables precise genetic modifications, revolutionizing disease treatment, crop improvement, and biotechnological innovation. VeCas9, a recently identified CRISPR endonuclease from the Veillonella genus, exhibits a broader protospacer adjacent motif recogniti...

Euthanasia and abandonment: Non-ideal theory, opportunity costs and financial incentives

Abstract: Recently, in Portugal, there have been several attempts by the parliament to pass a law that decriminalises euthanasia, and only the last one has passed. One of the arguments that have been raised against euthanasia, in the Portuguese context, is that euthanasia and med...

Ni–Zn–Ga Alloy for Weak Sunlight‐Driven Methanol Production From CO 2 and H 2 O at Ambient Pressure

ABSTRACT The sunlight‐driven artificial photosynthesis that converts CO 2 and H 2 O into methanol is a promising approach for carbon‐neutral fuel synthesis, but conventional systems require elevated pressure while producing methane by‐products, hindering practical operation. Here...

Integrated metabolomic and targeted lipidomic profiling reveals systemic metabolic dysregulation in the follicular microenvironment of polycystic ovary syndrome

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder that significantly impacts female fertility. The follicular microenvironment, particularly follicular fluid (FF), plays a critical role in oocyte development, yet the systemic metabolic dysregulation...

Human Subcutaneous Adipose Transcriptomes Link Mitochondrial Transcription to Metabolic Heterogeneity in Obesity

Background: Metabolically healthy obesity and metabolically unhealthy obesity may occupy different positions along a continuum of adipose-tissue dysfunction, but stable molecular distinctions remain uncertain. Methods: We integrated three public human subcutaneous-adipose-tissue...

Underestimated rice yield risks from extreme cold in China

Despite global warming, intensifying extreme cold anomalies present a significant paradox for global food security. While heat-related risks are well-documented, the impact of extreme cold on crop yields remains a critical yet understudied vulnerability in climate change assessme...

How Awareness of Media Slant Affects Reaction to News: Field and Experimental Evidence from Microblog Users

This paper investigates whether news consumers react strategically to media slant. Using data from Microblog users and an online experiment that varies whether sources and stances are disclosed, we find that users are more likely to repost news consistent with their own attitudes...

High-dimensional Clustering and Signal Recovery under Block Signals

This paper studies computationally efficient methods and their minimax optimality for high-dimensional clustering and signal recovery under block signal structures. We propose two sets of methods, cross-block feature aggregation PCA (CFA-PCA) and moving average PCA (MA-PCA), desi...

In Situ Genetic Engineering Facilitates MMP‐9‐Responsive TIMP1 Release Through Proteolytic Cleavage to Boost Diabetic Wound Healing

ABSTRACT Diabetic Foot Ulcer (DFU), a severe chronic diabetes complication with low healing and high recurrence rates, is a major global health challenge. Overexpression of matrix metalloproteinase‐9 (MMP‐9) and the consequent MMP‐9/TIMP1 (tissue inhibitor of metalloproteinase 1)...

Major Customer Network and Modern Slavery Incidents

ABSTRACT This study explores the impact of major customer network structure on the risk of modern slavery incidents. By focusing on the downstream segment of the supply chain, the study aims to clarify how relational dynamics with major customers shape a supplier's exposure to la...

Effectiveness of a Primary‐Care‐Centered Integrated Care Intervention for Stratified Cardiovascular Risk Control in China: Study Protocol for a Pragmatic Cluster Randomized Controlled Trial

ABSTRACT Introduction Gaps in primary care (PC) restrict its potential as a core foundation of the health system in its response to the growing burden of cardiovascular disease (CVD). China has been promoting County‐wide Tight Medical Alliances to strengthen PC by integrating ser...

Lithium chloride in vitro treatment shows potential to rescue the neuronal phenotype caused by WDFY3 haploinsufficiency

We provide a comprehensive phenotypic characterization of loss-of-function (LoF) variants in WDFY3 based on the largest cohort reported to date (n = 32). Our findings define a monogenic disorder marked by neuropsychiatric features (including autism and ADHD), mild to moderate neu...

Staged and Simultaneous Bilateral Hip Arthroscopy for Femoroacetabular Impingement Syndrome Achieves Clinical Outcomes Comparable With Unilateral Surgery at a Minimum 2‐Year Follow‐Up: A Systematic Review

Purpose To synthesize the available evidence comparing patient‐reported outcomes and reoperation rates between bilateral and unilateral hip arthroscopy in patients with femoroacetabular impingement syndrome, irrespective of whether bilateral procedures were performed in a staged...

Methyl-donor inhibitors relax 3D genome structures to promote totipotent reprogramming

The 3D genome safeguards cell identity while shaping developmental plasticity, yet its coordination with metabolism during cell fate transitions remains unclear. Here, we use a mouse totipotent stem cell induction system to dissect the role of methyl-donor metabolism in 3D genome...

Chemical-Delithiation-Induced Surface Reconstruction in Layered Oxide Cathodes for High-Performance All-Solid-State Batteries

Abstract Developing all-solid-state batteries (ASSBs) based on high-capacity cathodes (e.g., Ni-rich and Li-rich layered oxides) offers a promising route toward next-generation batteries with high energy density and safety. However, under high voltage, the cathode/solid electroly...

Femtosecond laser photoporation enables efficient delivery of apoptotic vesicles for enhanced osteogenesis

Abstract A major barrier to the translation of vesicle-based therapeutics in regenerative medicine is the ability to achieve efficient intracellular delivery without compromising, and ideally amplifying, biological activity. As a bioactive class of cell-derived vesicles with pro-...

Lightning Source of Nitrogen Oxides (NO x ) Inferred From Geostationary Satellites: Global Implications for Oxidant Chemistry

Abstract Lightning is a major source of nitrogen oxide radicals (NO x ≡ NO + NO 2 ), but large uncertainties in NO x yields per flash hinder accurate quantification of emissions and impacts on oxidant chemistry. We use 1 year of concurrent geostationary satellite observations of...

Assessing residual bias in real-world vaccine effectiveness studies: validating negative controls to avoid misleading results

Observational studies and randomized controlled trials demonstrate that vaccines protect against infections and severe outcomes, but real-world vaccine effectiveness estimates remain vulnerable to residual biases. Negative control outcomes can help detect and mitigate such biases...

Spin-Consistency Constraints in Noncollinear Tensor TDA

Abstract TDDFT for open-shell systems, whether spin-conserving or spin-flip, has long suffered from spin contamination. This problem arises because the single-excitation space built upon a single Kohn–Sham determinant is not spin-complete. Adopting spin tensor reference states th...

An infinite-times renewal equation

In neuroscience, the time elapsed since the last discharge has been used to predict the probability of the next discharge. Such predictions can be improved taking into account the last two discharge times, and preferentially more. Such multi-times renewal processes arise in many...