Research works

Homologous knock-in processes facilitated by CRISPR-Cas9 in Aurantiochytrium and disrupting the gene for DNA ligase IV prevents transformation events

Abstract This study explores advanced molecular breeding techniques for Aurantiochytrium limacinum , a eukaryotic microorganism valued for its industrial production of DHA and astaxanthin. We first employed the CRISPR-Cas9 system to simultaneously disrupt two genes: crtIBY , a mu...

The first steps after paralysis: Immediate locomotor responses following electrically induced loss and recovery of neuromuscular function

Abstract In nature, animals must rapidly regain coordinated locomotion after neuromuscular injury, but the earliest compensatory strategies remain unknown. We addressed this gap using a chronic, implantable kilohertz electrical stimulation (KES) system to reversibly block tibial...

Copper-Catalyzed Divergent1,4-Polyhaloalkylcyanationof 1,3-Enynes

Abstract We report a switchable copper-catalyzed divergent 1,4-polyhaloalkylcyanation of 1,3-enynes to construct a series of polyhalogenated allenes with high chemoselectivity and regioselectivity. The 1,4-dichloromethylcyanation of 1,3-enynes is realized by merging aminoalkyl ra...

A needle-mounted guide for improving needle visualization during ultrasound-guided needle insertion: a simulation study in novice operators

Abstract Background In ultrasound-guided needle insertion, continuous needle visualization is crucial for accurate needle placement, particularly for beginners. Novice operators may have difficulty maintaining the spatial relationship between the needle trajectory and the ultraso...

Dark Matter as a Screening Artifact of 5D Spacetime Dynamics

We propose an alternative framework for galactic rotation curves and gravitational dynamics without invoking particle dark matter, formulated within a 5-dimensional Kaluza-Klein scalar tensor geometry. By introducing a force-density screening mechanism acting as a dynamical self-...

Enhancing iNKT cell immunotherapy through the integration of optimized CAR endodomains and iNKT engagers

Abstract iNKT cells are emerging as a highly promising immunotherapy platform for the treatment of cancer. To maximise the anti-cancer activity of CAR-iNKT against the blood cancer multiple myeloma we investigated optimal CAR designs and their combination with new iNKT-specific e...

SEQUENCES WITH INEQUALITIES

Abstract We consider infinite sequences of positive numbers. The connection between log-concavity and the Bessenrodt–Ono inequality has been the focus of several papers. It has applications in the white noise distribution theory and combinatorics. We improve a recent result by Be...

Symmetric rendezvous on unlabelled complete graphs below the Anderson–Weber constant: certified manuscripts, exact-rational certificates, Lean 4 formalization, and independent audit layer

This record contains two companion manuscripts on symmetric rendezvous on unlabelled complete graphs, together with their complete reproducibility supplement Paper I (upper bound) constructs a legal symmetric strategy with lim E[T_n]/(n−1) = 0.82887837309423433534… < c_AW = 0.828...

Fiber Singularity Theory A Unified Framework of Fractal Geometry, Quantum Chaos, and Riemann ζ Zeros

Classical mathematics treats singularities (poles, essential singularities, discontinuities, non-differentiable points) as signs of theoretical failure. However, physical systems—from turbulence and black holes to quantum energy levels—frequently exhibit such structures, suggesti...

Integrative genomic and functional analyses identify LjbZIP36 as a candidate regulator of β-carotene accumulation during floral color transition in Lonicera japonica

Abstract Background Floral color transition is a characteristic developmental process in Lonicera japonica , during which flowers gradually change from white to golden yellow during flower maturation owing to carotenoid accumulation. Although carotenoid biosynthetic genes have be...

TSUMS MAX-STACK Full Replay Engine v1.0 Final: A Public-Layer Governance Lifecycle Replay and Cross-Ledger Compilation Framework

TSUMS MAX-STACK Full Replay Engine v1.0 Final is a Public-Layer governance lifecycle replay and cross-ledger compilation framework. It compiles known facts, missing records, duty signals, decision nodes, accountability mappings, stop-loss points, evidence chains, ledger bridges,...

E‑Spacetime: A Noncommutative Entanglement Geometry Extending Einstein Gravity

E‑spacetime is a noncommutative geometry defined by [T,Si]=Θi, where time–space entanglement generates curvature. This framework unifies geometry, fields, gravity, and particles, and recovers GR, QFT, and Yang–Mills theory in the commutative limit.

Facilitating advance care planning in oncology: study protocol for the evaluation of a tailored implementation of an advance care planning tool (the INDEED study)

Abstract Background Advance care planning (ACP) improves patient outcomes, but integration of ACP in daily practice for patients with cancer in hospitals remains suboptimal. Systematic implementation of tools to facilitate ACP could overcome the barriers for ACP. In this study we...

Bayesian Nonlinear Structural Model Parameter Updating Using Visual Damage Indicators

Abstract Postearthquake decisions regarding the repair, retrofit, and safety of damaged infrastructure often rely on accurate estimates of structural response demands. These estimates are highly sensitive to uncertain material parameters whose true values often deviate from those...

Solvent-Free Bubbling Extraction Mass Spectrometryfor Ultrasensitive, Size-Independent Detection of Microplastics inWater

Abstract Ultratrace microplastics (MPs) in aquatic environments are a growing problem for ecosystems and human health, yet their reliable detection at subng/L levels with sub-100 nm particle sizes remains a substantial analytical challenge. Herein, we introduce a solvent-free and...

The effect of health behaviour interventions on alcohol consumption among people living with cancer: a systematic review

PURPOSE: This systematic review synthesises data relating to the effectiveness of behavioural interventions aimed at reducing alcohol consumption among people living with cancer. METHODS: Five databases were systematically searched for randomised controlled trials (RCTs) publishe...

Medication transitions and predictors of illicit substance use during opioid agonist treatment: a three-year follow-up study

Abstract Background For opioid use disorder (OUD), pharmacological therapy using methadone or buprenorphine, plus psychosocial support, is the most effective opioid agonist treatment (OAT). Long-acting injectable buprenorphine (LAIB) is the newest buprenorphine formulation. This...

Spatial Regulation of Preameloblast Development in Response to Distal Mouse Incisor Injury

Enamel, the outermost mineralized tissue of the tooth, is produced by specialized dental epithelial cells called ameloblasts. Unlike human enamel, which lacks regenerative capacity, the mouse incisor grows throughout life, driven by adult stem cells residing in the labial cervica...

Physics-aligned causal inference of driving styles on electric vehicles battery aging

Abstract Lithium-ion batteries (LIBs) remain the core service life bottleneck of electric vehicles (EVs), and driving style is a critical human factor dominating EV battery degradation as assessed via aging proxy indicators and semi-empirical model predictions. Existing studies m...

Strict Total Positivity of Order Four for the de Bruijn--Newman Kernel

We prove that the de Bruijn--Newman kernel associated with the Riemann Xi function is strictly totally positive of order four. Let \[K(x,y)=\Phi(x-y),\] where \(\Phi\) is the classical positive even Riemann kernel. We establish that for every \[1\le m\le4,\] and every pair of str...