GPR35-positive mast cells are enriched in primary sclerosing cholangitis and show an enhanced secretory phenotype
Abstract
OBJECTIVE: dependent mast cell biology relevant for cholangitis and biliary fibrosis. METHODS: knockdown were determined by DESeq2 and gene set enrichment analysis. Selected cytokines, TNFα and IL13, were measured by qRT-PCR and ELISA. Mast cell-cholangiocyte interaction was studied in transwell co-culture with H69 cell line. RESULTS: dependent manner. CONCLUSION: supports a pro-secretory mast cell phenotype with potential relevance to biliary fibrosis. The increased infiltration of GPR35 positive mast cells in PSC near bile ducts could be a target for biliary fibrogenesis.
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Authors: Jørgen D. Rønneberg, Brian K. Chung, Xiaojun Jiang, Joshua E. Elias, Tom H. Karlsen, Nicole C. Kaneider, Johannes R. Hov, Georg Schneditz
Institutions: University of Cambridge, University of Oslo, Oslo University Hospital, Addenbrooke's Hospital, Cambridge School