伍蓉

Whole-genome sequencing of an in-house isolated Prevotella intermedia strain Pre.in-GSH

An anaerobic Prevotella intermedia isolate (strain Pre.in-GSH) was obtained from mouse fecal samples using selective culture-based isolation. Mice were orally administered with 3-methylhistidine (3-MH) for 7 consecutive days, and fecal samples were collected under sterile conditi...

Shotgun metagenomic profiling of gut microbiota in control and time-restricted feeding human cohorts

A prospective human cohort study was conducted to investigate the effects of time-restricted feeding (TRF) on the gut microbiota. Participants were assigned to either the control (CTRL) group or the TRF group. The TRF group underwent a 21-day time-restricted feeding intervention,...

Shotgun metagenomic sequencing of gut microbiota in control and time-restricted feeding mice

Time-restricted feeding (TRF) mice were allowed free access to food only between 10:00 and 18:00 each day for 2 weeks, whereas control (CTRL) mice had ad libitum access to food throughout the day. Fecal samples were collected from CTRL and TRF mice after the intervention and subj...

16S rRNA gene sequencing of gut microbiota from time-restricted feeding and fecal microbiota transplantation mice

Time-restricted feeding (TRF) donor mice were allowed free access to food only between 10:00 and 18:00 each day for 2 weeks, whereas control (CTRL) donor mice had ad libitum access to food throughout the day. Fecal samples from donor mice were collected and used to prepare fecal...

Liver transcriptomics of C57BL/6 mice after Prevotella intermedia or uric acid treatment with APAP challenge

C57BL/6 mice under control or time-restricted feeding were treated with Prevotella intermedia or uric acid, followed by acetaminophen (APAP) challenge. Liver tissues were collected for RNA sequencing to identify transcriptomic changes.

Untargeted fecal metabolomics of C57BL/6 mice after 14-day time-restricted feeding

C57BL/6 mice were subjected to a 14-day time-restricted feeding (TRF) regimen with a feeding window from 10:00 to 18:00. At the end of the intervention, fecal samples were collected and analyzed using untargeted metabolomics to profile metabolic alterations associated with TRF.

Untargeted fecal metabolomics of C57BL/6 mice after time-restricted feeding and Prevotella intermedia administration

C57BL/6 mice underwent a 14-day time-restricted feeding (TRF) regimen with a feeding window from 10:00 to 18:00. During the intervention, mice received oral gavage with Prevotella intermedia suspension on days 0, 1, 2, 5, 8, and 11-13. On day 14, fecal samples were collected and...

Fecal metabolomics of germ-free mice after 14-day time-restricted feeding

Germ-free mice were subjected to time-restricted feeding (10:00-18:00) for 14 days. Fecal samples were collected and analyzed by untargeted metabolomics to profile metabolic alterations associated with the intervention.

16S rRNA sequencing of fecal samples of time-restricted feeding in human

This study aims to evaluate the effects of time-restricted feeding (TRF) on the composition of the human gut microbiota. Fecal samples were collected after a 21-day TRF intervention and analyzed using 16S rRNA gene sequencing to assess microbial diversity and community structure.

16S rRNA sequencing of fecal samples after time-restricted feeding in mice

This study aims to investigate the impact of time-restricted feeding (TRF) on gut microbiota composition in mice. Fecal samples were collected after two weeks of TRF intervention and subjected to 16S rRNA gene sequencing to analyze microbial diversity and community structure.