Calr-mediated calcium buffering: a molecular barrier to in vivo cardiac reprogramming

Abstract

In a recent publication in Cell Stem Cell , Cai et al. 1 employed an in vivo Perturb-seq strategy to systematically identify molecular barriers that limit direct cardiac reprogramming following myocardial infarction (MI). This study provides a conceptual and technological framework for improving fibroblast-to-cardiomyocyte conversion in vivo and identifies a key regulatory pathway that can be targeted to enhance cardiac regeneration.