Second Affiliated Hospital of Zhejiang University

Methyl indole-3-acetate contributes to strain-specific anti-inflammatory effects of Lactiplantibacillus plantarum

Lactiplantibacillus plantarum is a widely recognized probiotic with anti-inflammatory potential, yet its efficacy varies among strains and the underlying determinants remain poorly defined. Here, we identify methyl indole-3-acetate (MIA), a previously uncharacterized microbial me...

ERK-dependent hyperexcitability of BLA neurons projecting to dCA3 underlies social dysfunction in a male mouse model of fragile X syndrome

Background Social dysfunction is a core symptom of autism spectrum disorder (ASD), including fragile X syndrome (FXS), but its underlying neural circuits and molecular mechanisms remain poorly understood. Previous studies have implicated the amygdala and hippocampus in social beh...

Cellular subpopulation remodeling and biphasic molecular dynamics in glioma recurrence: a multi-omics analysis and diagnostic markers screening using liquid biopsy samples

OBJECTIVE: Glioblastoma (GBM) is a highly aggressive brain tumor with a median survival of about 15 months and frequent recurrence. Liquid biopsy of cerebrospinal fluid (CSF) and plasma enables minimally invasive detection of tumor-associated molecular signals and may improve ear...

A tendon-targeted zwitterionic nanogel for restraining osteoclast maturation to halt heterotopic bone formation

Tendinopathy is a prevalent degenerative musculoskeletal disorder that can progress to heterotopic ossification (HO), ultimately resulting in tissue stiffening and biomechanical deterioration. However, effective early-stage therapeutic strategies to prevent pathological ossificat...

PANoptosis-related HLTF promotes prostate cancer progression and serves as a potential therapeutic target

Accumulating evidence indicates that pan-apoptosis plays a significant role in tumour progression, highlighting the importance of determining its relevance to tumour prognosis and treatment. This study aims to classify the molecular patterns of pan-apoptosis and explore the molec...

Unequal paths of healthy ageing: a qualitative exploration of urban-rural disparities in self-management of intrinsic capacity decline

Declining intrinsic capacity may compromise healthy ageing, while opportunities to manage functional change are shaped by social and structural circumstances. Urban-rural differences in the conditions surrounding everyday self-management remain poorly understood. This study explo...

Computed Tomography-Derived Sarcopenia and Two- Versus Three-Dimensional Body Composition for Prognostication in Colorectal Cancer Patients Receiving Chemoradiotherapy: An Automated Deep Learning Segmentation Study with External Reproducibility Assessment

Background: Skeletal muscle depletion predicts poor outcomes in gastrointestinal cancers, but whether volumetric three-dimensional (3D) body composition adds anything over the standard two-dimensional (2D) single-slice approach in colorectal cancer (CRC) patients receiving chemor...

Clinical application of systematic prostate biopsy combined with contrast-enhanced ultrasound guided targeted biopsy in patients with PSA gray zone

Abstract Objective To investigate the clinical value of combining systematic prostate biopsy with contrast-enhanced ultrasound (CEUS)-guided targeted biopsy in patients with serum prostate-specific antigen (PSA) levels in the gray zone. Methods A total of 167 patients in the PSA...

Efficacy and Safety of Recibokibart, an Interleukin-36 Receptor Antibody, in Generalized Pustular Psoriasis: A Phase 2 Randomized Trial

BACKGROUND: Generalized pustular psoriasis (GPP) is a rare, severe inflammatory skin disease characterized by acute flares. Recibokibart is a humanized IgG1 monoclonal antibody targeting the interleukin-36 (IL-36) receptor. In a phase 1b open-label study, a single intravenous dos...

Effects of ketogenic diet on obesity indices, lipid profile, and glycemic control parameters in athletes: a GRADE-assessed systematic review and meta-analysis of randomized controlled trials

The ketogenic diet has revealed more beneficial effects on metabolic health. However, its net effects on atherogenic lipoproteins, adiposity, and glycemic control remain inconsistent. This systematic review and meta-analysis aimed to quantify the pooled effects of the ketogenic d...

Active Learning‐Accelerated Discovery of Antifungal Polymers With Self‐Assembly Capacity for Tackling Invasive Fungal Diseases

ABSTRACT Invasive fungal diseases (IFD) are life‐threatening infections with limited clinical management. Antifungal peptide (AFP)‐mimicking polymers are promising candidates for tackling IFD. However, due to the structural diversity of polymers, it remains a great challenge to d...

Invasive candidiasis in the Intensive Care unit: diagnostic stewardship, source control, and precision antifungal therapy

INTRODUCTION: Invasive candidiasis remains one of the most challenging infections in the intensive care unit (ICU) because diagnosis is complicated by nonspecific clinical manifestations, frequent colonization, altered host immunity, complex anatomical sources and unstable pharma...

Application effect of flipped classroom combined with SNAPPS teaching model in emergency medicine interns

Abstract Objective To explore the application value of flipped classroom (FC) combined with SNAPPS teaching framework in emergency department clinical teaching for medical interns. Methods In this randomized controlled trial, a total of 104 fifth-year undergraduate medical intern...

Ultrasound-detected hepatic steatosis in normal-weight adults without diagnosed diabetes or conventional hypertension: burden and metabolic phenotype in 93,562 health examinees

Normal body mass index (BMI) may lower clinical suspicion of hepatic steatosis. We quantified the burden and metabolic phenotype of ultrasound-detected steatosis among normal-weight Chinese adults without diagnosed diabetes or conventional hypertension. This single-center cross-s...

Quality and reliability of osteoporosis-related health information on short-video platforms: a multi-source cross-sectional study

Abstract Background Osteoporosis (OP) affects approximately 145.86 million people in China, imposing substantial disease and societal burden. Effective management requires evidence-based patient education. Short-video platforms dominate health communication, yet no study has syst...

Small incision arthroscopically assisted disc anchorage of the anterior disc displacement of temporomandibular joint: part I - technique introduction

We describe a novel hybrid surgical technique, termed “small incision arthroscopically assisted disc anchorage” (SIAADA), for the treatment of anterior disc displacement (ADD) of the temporomandibular joint (TMJ). The aim of this study was to integrate the microinvasive benefits...

Brain calcification‐associated JAM2 pathogenic mutations impair JAM2 – JAM3 tight junction structure and the blood–brain barrier integrity

Abstract Primary brain calcification (PBC) is a neurodegenerative disorder characterized by bilateral brain calcification. JAM2 is a PBC causative gene, encoding the tight junction protein JAM2 and critical for blood–brain barrier (BBB) integrity, with an uncertain pathogenic mec...

A phase III clinical trial of PXT3003 in Chinese patients with Charcot-Marie-Tooth disease type 1A

Charcot-Marie-Tooth disease type 1A (CMT1A) is the most common inherited peripheral neuropathy and currently lacks disease-modifying therapy. PXT3003, a low-dose oral combination targeting PMP22 overexpression, has shown efficacy in two trials, while one recent confirmatory trial...

Metabolic-pathway-based classification of tendinopathy reveals a precise therapeutic strategy targeting chondro-modulatory tenocytes

Abstract Tendinopathy is a prevalent degenerative condition driven by complex metabolic dysregulation, yet its inherent metabolic heterogeneity remains poorly characterized, hindering targeted therapeutic development. To address this, we performed systematic multi-omics profiling...

Leptomeningeal fibroblasts promote glioblastoma progression by regulating cerebrospinal fluid dynamics

Abstract Cerebrospinal fluid contributes to homeostasis in the central nervous system, but how its dynamics are altered in glioblastoma is unclear. We find that glioblastoma drives leptomeningeal perivascular fibrosis that is associated with impaired fluid transport and clearance...