Mahidol University

Shared and Distinct T-Cell Receptor Characteristics of CD4+ and CD8+ Drug-Specific T-Cells In Vitro Generated from Healthy Donors and Hypersensitive Patients

Abstract The initiation of T-cell-mediated drug hypersensitivity reaction requires the formation of a human leukocyte antigen (HLA)-peptide-drug-T-cell receptor (TCR) complex. While specific interactions between HLA molecules and drug antigens are well-defined, the characteristic...

Global Prevalence and Specificity of Red Blood Cell Antibodies in Blood Donors: A Systematic Review and Meta-Analysis

Background: Red blood cell (RBC) antibody screening in blood donors is a key component of transfusion safety. However, reported prevalence and antibody specificities vary widely across studies, and a consolidated global estimate remains limited. This study aimed to quantify the p...

Single-cell morphological responses as predictors of ciprofloxacin resistance in Acinetobacter baumannii

ABSTRACT The rise of antimicrobial resistance (AMR) coincides with a declining pipeline of new antibiotics, underscoring the urgent need for rapid and accurate antibiotic susceptibility testing (AST). Single-cell AST approaches offer the potential to shorten diagnostic turnaround...

Human scFv inhibiting broad coronavirus replication: a pandemic preparedness candidate

The ongoing emergence of zoonotic coronaviruses (CoVs) and the rapid evolution of viral variants underscore the need for broadly active antiviral agents. The relatively conserved S2 subunit of the coronavirus spike protein is an attractive target for developing pan-coronavirus th...

Discovery of Bis-ThioureaDerivatives as Human DNATopoisomerase IIα Inhibitors with Potent Anticancer Effects

Abstract DNA topoisomerase IIα (Topo IIα) is essential for maintaining genomic stability during DNA replication and mitosis and is highly expressed in cancer cells, making it a promising target for anticancer therapy. In this study, bis-thiourea derivatives were investigated for...

When Clinicaland Metabolomics Data Work Together:A Comparative Framework for Multimodal Disease Classification acrossMachine and Deep Learning

Abstract Disease classification using clinical and metabolomics data increasingly relies on multimodal integration, yet the complementary and comparative contributions of these modalities remain poorly understood. Most existing frameworks prioritize predictive performance without...

Thai Modified Dental Anxiety Scale (Thai-MDAS): cross-cultural adaptation, psychometric validation, and preliminary diagnostic accuracy

Dental anxiety is a prevalent barrier to dental care worldwide. The Modified Dental Anxiety Scale (MDAS) is a widely used, brief self-report measure with established cross-cultural validity in numerous languages, yet no validated Thai-language version currently exists. This study...

Technical note: reconstructing dose distributions from manually planned electron boosts in breast radiotherapy

Purpose In breast radiotherapy, delivery of manually-calculated electron boosts limits retrospective dose–response analyses as dose distribution is unavailable. This work evaluates the feasibility of reconstructing dose distributions from manually planned electron boosts in breas...

Factors influencing the severity of road traffic accident injuries among pedestrians in Thailand: a national-level analysis

Objectives Road traffic accidents are one of the leading causes of injury and death compared to other causes. Pedestrians are highly vulnerable when they use the roads. When involved in road traffic accidents, pedestrians may experience dangerous, potentially fatal impacts when t...

Coronavirus disease 2019 vaccination and reduced severity-related outcomes in high-risk groups in Thailand during the Omicron period

Despite the coronavirus disease 2019 (COVID-19) booster vaccination, individuals with comorbidities continue to experience disproportionately severe COVID-19 disease and adverse outcomes. Evidence on associations between vaccination status and clinical outcomes among high-risk gr...

Modified nasopharyngeal airway device facilitates nasal fiberoptic intubation for trainee anesthesiologists: a randomized clinical study

Fiberoptic nasotracheal intubation is an essential skill for anesthesiologists. Trainee anesthesiologists often face challenges due to the complex anatomy of the nasopharynx and the risk of upper airway collapse after anesthesia administration, which can lead to confusion and dif...

Health Care Stigma and HIV/Sexually Transmitted Infection Prevention Service Uptake Among Cisgender Men and Gender-Diverse Individuals Who Have Sex with Men in Five Asian Countries: Implications for Differentiated Service Delivery

Health care stigma remains a key barrier to HIV and sexually transmitted infection (STI) prevention, yet its effects may differ across health care systems. We examined associations between health care stigma and HIV/STI prevention service uptake across five Asian countries. A cro...

Phenotypic and Genomic Analysis of Swine-Derived Bacillus subtilis 1BP1Co1 as a Functional Probiotic Candidate to Mitigate Escherichia coli-Associated Diarrhea

Post-weaning diarrhea caused by pathogenic Escherichia coli is a significant economic challenge in global swine production. Rising antimicrobial resistance linked to traditional antibiotics necessitates safe, sustainable alternatives. This study isolated and characterized host-as...

ZPY-CpG-Ch, a broad coverage vaccine against Streptococcus pneumoniae

Existing licensed pneumococcal vaccines, including Prevnar-13 and its higher-valency successors, have played a major role in reducing invasive pneumococcal disease worldwide. However, their effectiveness is increasingly threatened by the rise of multidrug-resistant strains and th...

Terminology for describing the 24-h cycle of physical activity, sedentary behaviour, and sleep: a scoping review

Abstract Background Physical activity, sedentary behaviour, and sleep are three behaviours which occupy the 24-h cycle and interact to influence a multitude of health outcomes. Different terms are currently used to describe these behaviours collectively (e.g., movement behaviours...

PARP inhibition induces a redox-dependent dormant reservoir vulnerable to disulfiram in high-grade serous ovarian cancer

Resistance to poly(ADP-ribose) polymerase inhibitors (PARPi) in high-grade serous ovarian cancer (HGSOC) is increasingly driven by non-genetic drug-tolerant dormant cell populations, yet the adaptive programs sustaining these cells remain poorly understood. Here, we established a...

Ruling In and Ruling Out Sepsis Using Likelihood Ratios of a Host Response Assay

Overview: SeptiCyte RAPID is an FDA-cleared gene expression test that quantifies host immune response to aid in the diagnosis of sepsis. The test yields a score (the SeptiScore) ranging from 0–15, distributed across four bands (1–4) based on increased likelihood of sepsis. Each b...

Clinical and Immunological Responses to Dermatophagoides farinae Allergen Immunotherapy in Dogs With Atopic Dermatitis

ABSTRACT Background Atopic dermatitis (AD) in dogs is a prevalent allergic disorder associated with environmental allergens, including the house dust mite Dermatophagoides farinae (Df). Hypothesis/Objectives To describe clinical and immunological changes over time in dogs with ca...

Evaluation of recombinant TgGRA14 as a diagnostic antigen for detection of Toxoplasma gondii antibodies in cattle

Toxoplasma gondii ( T. gondii ) a widespread zoonotic parasite infecting various host species. Since cattle serve as major intermediate hosts for T. gondii , giving a significant public health concern, developing effective diagnostic tools is essential. This study evaluated the s...

Incorporating Naive Bayes classification to address subpopulation structure in familial DNA search

Familial DNA search evaluates the genetic relatedness of two individuals by comparing the likelihood of their observed DNA profiles under two competing hypotheses-the null hypothesis that the individuals are unrelated and the alternative hypothesis that they are related-most comm...