Leiden University Medical Center

International Society of Urological Pathology Multidisciplinary Expert Consultation Conference on Evaluation of Treatment Effects in Prostate and Bladder Cancer

Treatment effects in the urinary bladder are variable and range from localized post-transurethral resection changes to widespread systemic therapy effects. The International Society of Urological Pathology (ISUP) organized a consensus meeting in Vienna, Austria, in September 2025...

HLA class I expression and tumor immune infiltration together shape colon cancer immune contexture

Background The relative contribution of HLA class I molecules—including classical HLA class Ia (HLA-A, -B, -C) and non-classical HLA class Ib (HLA-E, -F, -G)—to shaping the tumor immune microenvironment in colon cancer remains insufficiently defined. We investigated how their exp...

Guidance for laboratory implementation, governance and continuous assurance of artificial intelligence in histopathology

Abstract Artificial intelligence (AI) is rapidly transforming histopathology, with applications ranging from workflow optimisation and quality assurance to tumour diagnosis, grading, biomarker assessment and estimation of prognosis. While numerous AI algorithms have demonstrated...

Time-dependent prognostic value of coronary plaque burden and hyperemic myocardial blood flow

Abstract Rationale The time-dependent contributions of coronary atherosclerotic plaque burden and myocardial ischemia to clinical outcomes in suspected coronary artery disease (CAD) remain uncertain. This study aimed to evaluate the temporal associations of quantitatively assesse...

Timing of PCI in Patients Undergoing Transcatheter Aortic-Valve Implantation

BackgroundApproximately one half of patients undergoing transcatheter aortic-valve implantation (TAVI) have concomitant coronary artery disease. Although percutaneous coronary intervention (PCI) is often performed before TAVI, the preferred treatment strategy has not been establi...

LDL Cholesterol Lowering With Evolocumab Before Percutaneous Coronary Intervention for Acute Myocardial Infarction

Importance: Proprotein convertase subtilisin-kexin type 9 (PCSK9) inhibition is recommended as second- or third-line lipid-lowering therapy after myocardial infarction (MI), resulting in prolonged periods of inadequate low-density lipoprotein cholesterol (LDL-C) control. Whether...

Nanobodies specific for Vβ11 enable detection and noninvasive imaging of antigen-specific T cells in vivo

Through screening of a VHH phage display library constructed from an alpaca immunized with the recombinantly expressed murine myelin oligodendrocyte (MOG)-specific 2D2 T cell receptor (TCR), we identified nanobodies that reduced antigen-driven 2D2 T cell activation in vitro. Immu...

Expected costs and benefits of genetic counselling and germline genetic testing in metastatic prostate cancer

Abstract Identifying pathogenic germline variants in men with metastatic prostate cancer is important for therapeutic options and for identifying relatives who may have a high cancer risk. To keep genetic testing costs manageable, it is important to identify which patients should...

Motivational orientation and influencing factors of older adults with chronic obstructive pulmonary disease in community dwellings: a latent profile analysis

Chronic obstructive pulmonary disease (COPD) poses a serious threat to older adults’ health. Promoting the adoption of health behaviors among community-dwelling patients is essential for improving prognosis, with motivational orientation serving as a key determinant of health beh...

DNA end-resection is stimulated by an interaction between BRCA1 exon 11 and TOPBP1

Abstract Approximately 60% of the tumour suppressor protein BRCA1 is encoded by a single exon. Many tumours carry mutations in this exon, often resulting in exon skipping and thus a protein of a severely reduced size. Although none of the well-described protein domains of BRCA1 a...

Conserved structural features of RNA export pores spanning the double membrane of arterivirus and coronavirus replication organelles

ABSTRACT Coronaviruses and arteriviruses are distantly related positive-stranded RNA virus families within the order Nidovirales . Both transform intracellular membranes into double-membrane vesicles (DMVs) that serve as replication organelles. Newly made viral RNA presumably exi...

Beyond benchmarking: an expert-guided consensus approach to spatially aware clustering

Spatial omics technologies have revolutionized the study of tissue architecture and cellular heterogeneity by integrating molecular profiles with spatial localization. In spatially resolved transcriptomics, delineating higher-order anatomical structures is critical for understand...

Impact of Charlson Comorbidity Index conditions on one-year survival in patients with breast cancer: a Danish population-based cohort study

Breast cancer is the most frequently diagnosed malignancy in women. The Charlson Comorbidity Index (CCI) is a widely used tool for quantifying multimorbidity. As the population ages, breast cancer incidence and comorbidity prevalence are expected to grow. We therefore investigate...

Four new Duchenne muscular dystrophy mouse models with clinically relevant exon deletions in the human DMD gene

Abstract Mutation specific therapeutic approaches, like exon skipping or gene-editing, hold promise for the treatment of Duchenne muscular dystrophy (DMD). Translatability of preclinical studies investigating these approaches could greatly be improved through the use of humanized...

Parent Mental Health From Preterm Birth Through the NICU Stay

OBJECTIVES: Preterm birth and neonatal intensive care unit (NICU) admission are not only extremely stressful for the infant itself but can also impact parental mental health. This Dutch national prospective cohort study aimed to describe the trajectory of parental mental health f...

The Moral (Un)Desirability of New Biomedical Technologies as Potential Techno‐Fixes

ABSTRACT The techno‐fix argument, used to argue against technological interventions that address the consequences of social problems instead of their underlying causes, is increasingly invoked in biomedical discussions. However, especially in biomedicine, the techno‐fix argument...

An ecosyndemic framework for understanding obesity: Spatial clustering of health, environmental and socioeconomic disadvantage in the Netherlands

Abstract Introduction Obesity and related health conditions are unevenly distributed across neighborhoods, often co-occuring with multiple health challenges and socioeconomic disadvantages. Using an ecosyndemic framework, which integrates ecological and social dimensions that con...

The Dutch Prolactinoma Cohort Studies: Cross-sectional Analysis of Females Focusing on the Patient's Perspective

Abstract Purpose To assess the results of patient-reported outcome measures (PROMs) in a national cohort of females with a prolactinoma by disease activity. Methods Females with prolactinoma included in the PRolaCT or ProlaC study were assessed cross-sectionally, comparing patien...

Clinical DVH metrics as a loss function for 3D dose prediction in head and neck radiotherapy

Abstract Purpose Deep learning-based three-dimensional (3D) dose prediction is widely used in automated radiotherapy workflows. However, most existing models are trained with voxel-wise regression losses, which are poorly aligned with clinical plan evaluation criteria based on do...

Defining global priorities in hematopoietic transplantation and cellular therapy: a statement paper from EBMT Global ComMittee

Abstract Hematopoietic cell transplantation, cellular and gene therapies, and (CAR)-T cells have revolutionized outcomes in hematologic disorders. However, major disparities persist in their availability, accessibility, and affordability, particularly in low- and middle-income co...