Leiden University Medical Center

Direct probabilistic IMPT treatment planning with setup and range errors for neuro-oncological patients

Abstract Objective . To show computational feasibility of a previously proposed probabilistic planning approach on a neuro-oncological patient group, we compared probabilistic plans with (automated) robust plans for one patient (group A) that could achieve sufficient clinical tar...

Chemogenetic ubiquitin E1 inhibitor TAK-243 CRISPR screen uncovers HAPSTR1/HUWE1 axis in chromatin proteostasis and cell cycle control

In this study, we sought to comprehensively map ubiquitin signaling networks at the crossroads of cellular proliferation and proteostasis, both crucial in cancer. Genome-wide CRISPR knockout screening in the presence of the ubiquitin E1 inhibitor TAK-243 identified 239 genes whos...

Paediatric haematopoietic stem cell transplantation research priorities: Results from an international multi‐stakeholder priority setting partnership

Summary Co‐designed research in paediatric HSCT is limited. We sought to determine research priorities which represent the shared priorities of patients, parents, carers and healthcare professionals (HCP) within Australia, New Zealand, the Netherlands and the United Kingdom. An i...

Home-based geriatric rehabilitation after shortened inpatient rehabilitation: cost-effectiveness analysis of the “Better@Home” multicentre prospective cohort study with a matched historical control group

PURPOSE: Geriatric rehabilitation (GR) is facing increased demand due to population ageing. Home-based GR could reduce reliance on resource-intensive inpatient care. However, evidence is limited regarding the cost-effectiveness of home-based GR. The aim of this study is to assess...

Pimicotinib versus placebo in tenosynovial giant cell tumor (TGCT): a plain language summary of the MANEUVER clinical trial

Plain Language SummaryWhat is this summary about?This summary describes the main findings of the MANEUVER clinical trial for pimicotinib, a once-daily treatment. The goal was to see if pimicotinib could significantly reduce tumor size after 24 weeks of treatment in clinical trial...

Cholangioscopy in the diagnostic and therapeutic work-up of intraductal papillary neoplasms of the bile duct: a systematic review and meta-analysis

Background: Intraductal Papillary Neoplasms of the Bile Duct (IPNB) are rare and diagnostically challenging, particularly in assessing invasiveness and intraductal spread. Cholangioscopy offers promise through direct visualization and targeted biopsies. The aim of this study was...

Exploring transcriptomic and genomic latent variable correction approaches in differential expression analysis

Abstract Background Differential expression analysis is a central tool for studying the biological processes altered in human diseases via transcriptomic signatures. However, transcriptomic datasets are systematically confounded by latent variables from two distinct sources: unme...

Incidence of recurrent major bleeding and its impact on survival in LVAD patients: a multistate analysis

ABSTRACT Introduction Major bleeding frequently complicates the clinical course of left ventricular assist device (LVAD) therapy. However, data on recurrent major bleeding in destination therapy (DT) patients, as well as its impact on survival, is limited. Methods: Single-center...

Chronotherapeutic considerations for antiplatelet therapy in cardiovascular risk prevention – A systematic review

Summary: Background: Cardiovascular physiology exhibits circadian rhythms, including a morning surge in platelet reactivity and cardiovascular events. Aligning the timing of antiplatelet therapy with these rhythms may be a simple, cost-effective approach to improve clinical effic...

ExonViz: Transcript visualization made easy

ExonViz is a simple tool to draw transcripts that include coding and non-coding regions. All exons are to scale and the reading frames of the exons are visible. Single or multiple variants along the transcript can be drawn and will automatically be shown in the correct location.

Diagnostic value of fluorine-18-fluorodeoxyglucose positron emission tomography/computed tomography in staging histological subtypes of invasive bladder cancer

Abstract This study assessed the diagnostic value of [ 18 F]FDG-PET/CT in staging histological subtypes (HSs) of invasive bladder cancer. In 112 patients with ≥ 75% HS-content, 87% of primary tumours were FDG-avid, 9% were non-assessable (due to high FDG-avid urine), and only 5%...

Infection of macrophages by mpox virus activates inflammasome and cell death, informing multitarget therapeutic strategies

Severe mpox virus (MPXV) infection causes immunopathological manifestations in patients. Here, we provide clinical evidence of MPXV-induced inflammation in a large patient cohort and demonstrate direct macrophage infection in skin tissues from an autopsied mpox patient. In human...

Cutaneous Lymphoma International Prognostic Index and Risk Stratification in Advanced Mycosis Fungoides and Sézary Syndrome

Importance Risk stratification in advanced mycosis fungoides and Sézary syndrome (MF/SS) remains suboptimal given that current TNMB (tumor, node, metastasis, blood) staging has limited prognostic value for clinically relevant outcomes. Objective To determine whether the Cutaneous...

Core outcome set for quality of life of family caregivers of adults with epilepsy: A Delphi consensus study

OBJECTIVE: Epilepsy significantly affects daily functioning and health-related quality of life (HRQOL) of those living with the condition. Less is known about HRQOL of family caregivers of adults with epilepsy, yet the functioning of patients and caregivers is interlinked, and ca...

Liver X receptor (LXR) signaling in macrophage inflammation and cardiometabolic disease.

Cardiometabolic diseases profoundly remodel tissue immune landscapes, characterized by the accumulation of diverse macrophage subtypes. Among these, lipid-associated macrophages (LAMs) have emerged as an important subset that is frequently observed in cardiometabolically affected...

Mapping the Intellectual Structure of Green-Synthesized Nanoparticle Research in Dentistry: A Bibliometric Analysis

Objective: Research on green-synthesized nanoparticles in dentistry has grown rapidly, yet remains synthesized mainly through narrative and scoping reviews without formal quantitative mapping. This study characterized publication trends, thematic clusters, geographic productivity...

Gut resilience and clinical homeostasis following administration of neomycin and albendazole in soil-transmitted helminth-infected adults living in Lambaréné, Gabon

Abstract Purpose Gut resilience and systemic homeostasis are interrelated dynamic functions. A gut that fails to regulate intestinal barrier integrity, metabolite production, and immune regulation will lead to systemic pathogenic invasion and susceptibility to infections. Gut res...

Toward the Use of Real‐World Data for Regulatory and HTA Decision‐Making: Experiences and Recommendations from Six EU ‐Funded Projects

Real-world data are increasingly important for regulatory and health technology assessment decision-making, yet their effective use across Europe remains limited by technical, methodological, regulatory, and societal challenges. Drawing on the experiences of six Horizon Europe pr...

Leveraging the wearable 1-lead ECG signal. From cardiac rhythm to cardiac function assessment

Abstract Traditional 12-lead ECGs offer comprehensive insights into the electrical activity of the heart, but require clinical settings and expert interpretation, which limits their accessibility. Smartwatch 1-lead ECGs can be recorded at home, allowing more frequent and rapid mo...

EXPRESS: Bayesian networks to estimate prognosis in vascular cognitive impairment and small vessel disease: integrated analyses of interdependent contributors to multiple outcomes

BACKGROUND: Vascular cognitive impairment (VCI) and small vessel disease (SVD) involve interconnected factors influencing multiple outcomes, also beyond cognitive decline. Bayesian networks (BNs) can help unravel these complex interrelations, which we demonstrate in this proof-of...