Leiden University Medical Center

Ten-year insert wear and the migration of uncemented tibial and femoral components with either conventional polyethylene or sequentially annealed highly crosslinked polyethylene: a blinded randomized controlled trial using radiostereometric analysis

Aims The aims of this randomized controlled trial (RCT) was to compare the long-term insert wear and migration of the tibial and femoral components between highly crosslinked polyethylene (HXLPE) inserts and conventional polyethylene (PE) inserts in an uncemented Triathlon fixed...

Implementing environmentally sustainable behavior in healthcare: an illustration of why behavioral definitions matter

Abstract Background Implementing more sustainable practices is crucial for reducing the environmental footprint of the healthcare sector. While organizational factors such as availability of sustainable materials are important, healthcare professionals’ adoption of sustainable be...

Insulin Resistance in the 21st Century: New Takes on an Old Problem—2025 Diabetes , Diabetes Care , and Diabetologia Expert Forum

Insulin resistance is a hallmark of type 2 diabetes and several other metabolic diseases. However, efforts to improve insulin resistance are hindered by the biological complexity inherent to insulin action, as well as conceptual and methodological limitations to date. In Septembe...

Insulin resistance in the 21st century: new takes on an old problem—2025 Diabetes, Diabetes Care, and Diabetologia Expert Forum

Insulin resistance is a hallmark of type 2 diabetes and several other metabolic diseases. However, efforts to improve insulin resistance are hindered by the biological complexity inherent to insulin action, as well as conceptual and methodological limitations to date. In Septembe...

The impact of physical activity and exercise timing on metabolic health among individuals at high risk of type 2 diabetes

The timing of physical activity and exercise has been suggested to play a role in metabolic health. This narrative review synthesises current evidence on the impact of habitual physical activity and exercise timing on glucose homeostasis and insulin sensitivity, blood lipids and...

Cardiac Troponin T Stability Study for Regulatory Approval of the 6th Generation High-Sensitivity Assay.

BACKGROUND: High-sensitivity cardiac troponin T (hs-cTnT) assays must meet strict criteria prior to regulatory approval, as the test is central to myocardial infarction diagnosis. Evaluating analyte stability is essential for ensuring accurate results in stored samples. Here, cTn...

Ancillary testing in paediatric non-CNS solid tumours: a real-world comparison with WHO criteria

Aims Paediatric tumour pathology poses diagnostic challenges due to morphological variation, overlap and the rarity and diversity of neoplasms, for which the WHO provides a comprehensive diagnostic framework. In clinical practice, however, diagnostic reasoning starts with an unkn...

Evaluation of nationwide variation in post-haemorrhagic ventricular dilatation management following national guideline implementation in the Netherlands

Abstract Purpose Post-haemorrhagic ventricular dilatation (PHVD) is a severe complication of germinal matrix–intraventricular haemorrhage in preterm infants and is associated with significant morbidity and adverse neurodevelopmental outcomes. In 2023, national recommendations for...

Molecular imaging tracers targeting c-MET: a systematic review of preclinical evidence and clinical translation

INTRODUCTION: The mesenchymal-epithelial transition factor (c-MET) is an emerging target for molecular imaging owing to its widespread overexpression across multiple cancer types. This systematic review evaluated radioactive and fluorescent c-MET-targeting tracers in preclinical...

Cognitive learning strategy use and their combinations in first year biomedical programmes

Effective learning strategies enhance long-term learning and academic performance, and are important for lifelong learning. However, previous studies are inconsistent and show substantial differences in use and effectiveness dependent on level of education and learning domain. Ou...

Adherence to treat-to-target recommendations in juvenile idiopathic arthritis in clinical practice

Abstract Objective To assess adherence to Treat-to-Target (T2T) recommendations for juvenile idiopathic arthritis (JIA) in clinical practice. Methods A nested cohort study in the multicenter international prospective UCAN JIA cohort was performed. Patient characteristics, clinica...

The Many Faces of Contrast-Associated Encephalopathy: A Systematic Review

Abstract Objectives The increased use of endovascular procedures shows an increase of neurological deficits after intravascular administration of iodine-based contrast media (ICM). Unfortunately, nonuniform terminology is confusing and may explain low awareness of this ICM-associ...

Selective immunoglobulin A deficiency and coeliac disease: Immune response dynamics

Abstract Objective To analyse the serological immune response in paediatric patients with selective immunoglobulin A deficiency (SIgAD) and coeliac disease (CeD), focusing on immunoglobulin G antibody dynamics after starting the gluten‐free diet (GFD). Methods Data from SIgAD pat...

Comprehensive In Vitro Profiling Demonstrates Successful Immune Evasion of Bioengineered Immune‐Shielded Heart Valves

Living donor valves for valve replacement in pediatric patients, come with the risk of immune rejection of the implanted tissue. To address this, and increase the options for living implants in pediatric patients, we previously developed a lentiviral immune-shielding strategy tha...

Filling the gaps in high-priority reference materials for implementing metrological traceability in laboratory medicine

Abstract The global harmonization of measurements in laboratory medicine is facilitated by the development and implementation of reference measurement systems, including suitable certified reference materials (CRMs). There are, however, a relatively small number of CRM producers...

Integrating optimal patient-centered care: patient- and family-reported experiences after complex treatment for rare diseases

Abstract Background Although life expectancy after complex treatments for rare diseases during childhood has increased, long-term medical, psychosocial and societal impacts remain for children and their families. Evaluating patient and family experiences is essential for the deve...

Early identification of individual, parent and family related factors associated with different levels of youth care services

Abstract Intensive youth care (out-of-home care and repeated outpatient care) is often provided to children with complex and persistent difficulties and is associated with adverse later outcomes. In order to reduce the escalation of care needs by timely referring to lighter forms...

Survival benefit of deceased donor kidney transplantation versus continued dialysis: international target trial emulation

Abstract Objective To quantify the survival benefit of deceased donor kidney transplantation versus continued dialysis according to patients’ characteristics (age, diabetes, cardiovascular disease), donor quality (standard criteria donor or expanded criteria donor) and donor retr...

Proximity biotinylation at the host-Shigella interface reveals UFMylation as an antibacterial pathway

Abstract Host cells contest invasion by intracellular bacterial pathogens with multiple strategies that recognise and/or damage the bacterial surface. To identify host defence factors targeted to intracellular bacteria, we developed a proximity biotinylation approach coupled to q...

Associations of frailty and COVID-19 severity with long-term health outcomes of COVID-19 among older people

To investigate whether frailty and COVID-19 severity are associated with long-term health outcomes of COVID-19 in older people. In this cohort study, older people aged ≥ 70 years with COVID-19 between January 2020 and December 2021 were invited for follow-up. Participants were re...