Research works

Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines

Pancreatic ductal adenocarcinoma (PDAC) cell lines resist transfection, limiting gene-editing experiments. Here, we present a protocol for delivering CRISPR-Cas9 or prime editors into PDAC cell lines using retrovirus-derived engineered virus-like particles (eVLPs). We describe st...

Triglyceride-high-density cholesterol-glucose-body index and risk of impaired fasting glucose in normolipidemic adults: a secondary analysis of a multicenter cohort

The triglyceride-high-density cholesterol-glucose-body index (TyHGB) is a composite index incorporating routinely measured lipid, glucose, and anthropometric parameters. However, its longitudinal association with the risk of impaired fasting glucose (IFG), a critical prediabetic...

Cancer Cell Membrane-Biomimetic Nanobubbles Carrying Indocyanine Green for Ultrasound/Fluorescence Bimodal Imaging of Colorectal Cancer

Abstract The aim of this study was to construct cancer cell membrane-coated and Indocyanine green-loaded nanobubbles for targeted ultrasound/fluorescence bimodal imaging of colorectal cancer (CRC). ICG-loaded nanobubbles (ICG-NBs) and CT26 (a murine CRC cell line) cancer cell mem...

Recent concepts in biological augmentation for rotator cuff tears: a scoping review

Surgical repair of rotator cuff tears is common, but failure rates remain a significant clinical challenge, particularly in large or degenerative tears. Augmentation strategies have been developed to enhance the biological and mechanical environment of the repair, aiming to impro...

Interval-dependent maintenance policy optimisation of a system subject to multi-level preventive maintenance types: a non-parametric, severity-aware approach

Purpose This paper develops a unified framework for preventive maintenance (PM) modelling that simultaneously accounts for downtime severity and interval-dependent effectiveness. The aim is to generate maintenance policies that are both risk-aware and operationally efficient for...

Integrated mRNA – miRNA - metabolite landscape revealed developmental and environment-driven regulation of curcumin biosynthesis in Lakadong turmeric

The Lakadong turmeric variety of Meghalaya is globally known for its high curcuminoid content in its rhizome. However, the framework to understand its genotype & environment driven interaction with that of molecular regulatory network is little known. In this context, we performe...

Why are tourists willing to wait longer in theme parks? The positive effects of narratives and destination brand love

Purpose This study examines why tourists are willing to incur additional waiting time for a preferred theme park experience rather than choose comparable alternatives involving shorter waits. Design/methodology/approach Zootopia at Shanghai Disneyland was selected as the research...

VERA-SE: Verification-Guided Autonomous Software Repair with Risk-Aware Patch Selection

VERA-SE presents a verification-guided autonomous software repair framework that combines large language model (LLM)-based patch generation with structural analysis, behavioral preservation checks, change-risk estimation, and risk-aware patch selection. The framework separates ca...

Long-Term Survival of a Maxillary Subperiosteal Implant After Blast Injury: A 17-Year Follow-Up Case Report

Abstract Subperiosteal implants (SPIs) have re-emerged as a treatment option for the rehabilitation of patients with severe maxillary atrophy, although evidence on their long-term performance remains limited. We report the case of a 53-year-old female patient who underwent maxill...

The Universemodel Protocol v2.41: A Cosmostatic Superfluid Framework for Microcosm and Macroscale Physics. Addenda F–I: Particle Genesis, Wave Scaling, and Femtosecond Annihilation Dynamics.

This paper introduces the second series of systematic extensions (Addenda F–I) to the Universemodel Protocol v2.41, further unifying sub-atomic phenomena and solid-state mechanics under a deterministic fluid-pressure framework. By utilizing the Universal Hydraulic Master Equation...

Who Was Before Me, or Myself? On the Three Questions Hidden in "Before," the Self Without an Earlier Self, and the Beginning That Is Not a Before

The chicken-and-egg paradox appears to ask which of two beings came first, but its grammar conceals two different relations: the developmental relation of one subject to its own earlier phase, and the reproductive relation between numerically distinct subjects. This essay develop...

Working Conditions and Welfare of Adjunct Faculty in Liberian Universities: A Qualitative Study

This study examines the working conditions of adjunct faculty in selected Liberian universities, focusing on employment security, remuneration, institutional resources, and opportunities for professional and scholarly development. The study was guided by Conservation of Resources...

Beyond a Single Risk Category: Within-Group Correlates of Bullying Victimization and Suicidality in Transgender Adolescents

Aim: Transgender adolescents experience elevated rates of bullying and adverse mental health outcomes compared with cisgender peers, yet most research has treated this group as homogeneous. We aimed to identify sociodemographic and physical health correlates of bullying victimiza...

Metabolic syndrome before 16 weeks’ gestation: prevalence and haemodynamic profile in a socioeconomically disadvantaged pregnancy cohort: baseline findings from Mother’s Heart Study

Abstract Background Pregnancy provides an opportunity to identify young women at risk of future cardiometabolic diseases. We aimed to determine the prevalence of metabolic syndrome (MetS) and its components, and to assess haemodynamic profiles in early pregnancy. Method We recrui...

Charting brain structure in 22q11.2 deletion syndrome with clinical neuroimaging

Abstract Background 22q11.2 deletion syndrome (22q11DS) is a common microdeletion associated with widespread brain alterations and elevated risk for schizophrenia and other neuropsychiatric conditions. Prospective research studies often exclude individuals with severe cognitive i...

Determination of Chlorination Disinfection By-products on Healthcare Environmental Surfaces by Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry

Chlorine-based disinfectants are standard for environmental-surface disinfection in healthcare facilities, yet their reaction with organic residues generates disinfection by-products (DBPs) whose occurrence, levels, and occupational relevance on hospital surfaces remain uncharact...

How Nespresso transformed from a product-centric business to an AI-driven service platform – and which business model innovation factors made it succeed

Purpose This real impact article focuses on the interplay between business model innovation and artificial intelligence (AI) in the coffee industry. We explored Nespresso's omnichannel customer engagement as a key case study on AI-enabled platform service model. Drawing on second...

Knowing the Norms: Regulatory-Readiness Engineering for a Physician-Governed Clinical AI System — Traceable Evidence and Declared Gaps

Part III of Volume II (the outward extensions) of the H-DKPS series on physician-governed clinical AI (知衡八法). The redacted code package is included as a separate file. Clinical problem. A physician who builds clinical software for use in their own clinic will sooner or later...

CLINICAL EFFECTIVENESS OF A HEMODYNAMIC RISK-BASED PERSONALIZED PROTOCOL DURING COMBINED SPINAL-EPIDURAL ANESTHESIA

Objective. To evaluate the clinical effectiveness of a personalized management protocol based on preoperative stratification of hemodynamic instability risk in multiparous pregnant women with anemia undergoing cesarean delivery under combined spinal-epidural anesthesia. Materials...

Book-Balancing Magic Has Nowhere to Hide: Source-Native Accounting and Actual Continuation

We construct a whole-ledger continuation chain fulfilled by one actual source: an original residual in the complete future model generates the syntax and responsibility row of the next request; the source program executes and strictly pays the same debt; and its completion event...