Histological and Biomechanical Considerations of Donor Costal Cartilage Allografts for Total Ear Reconstruction
Abstract
ObjectiveDonor costal cartilage (DCC) allografts for microtia reconstruction have shown promising clinical results, but characterization of the allografts' intrinsic properties has yet to be performed.DesignHistologically, DCC allografts were compared to autologous costal cartilage (ACC). Biomechanical properties of eight DCC were compared with normative literature values.SettingUniversity laboratory.Patients, ParticipantsAdolescent and teenage patients undergoing microtia/anotia reconstruction.Main Outcome MeasuresHistological comparisons between DCC and ACC were performed using Safranin O, Alcian Blue, Masson's Trichrome, and Collagen II-specific staining. Biomechanical evaluation of DCC yielded Young's modulus, ultimate tensile strength, and ultimate compressive strength.ResultsHistologic analysis demonstrated preservation of most collagen content, with a notable reduction in the glycosaminoglycan content of the extracellular matrix in DCC. DCC demonstrated biomechanical performance that overlaps with ACC based on reported literature values.ConclusionsThe histologic integrity and biomechanical characteristics reveal DCC may have structural integrity that resembles ACC.
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Authors: Arya Sherafat, Lilian Doan, Ashton Rosenbloom, Ryan P. Donahue, Shingirirai Michael Chaokma, Kyriacos A. Athanasiou, Mary Ziegler, Alan Widgerow, Rahim Esfandyarpour, Miles J. Pfaff
Institutions: University of California, Irvine, University of California, Irvine Medical Center, University of California, Riverside, Children's Hospital of Orange County, NewYork–Presbyterian Brooklyn Methodist Hospital, Tissue Genetics (United States)