Low miR-106a-5p is associated with burn-related DVT and may regulate endothelial injury via TXNIP

Abstract

Among burn patients, deep vein thrombosis (DVT) frequently arises as a critical complication. This article aims to explore the mechanism of miR-106a-5p in DVT in burn patients, providing a basis for identifying early diagnostic markers and developing therapeutic targets. This study selected clinical data from 228 burn patients (DVT, n = 108; non-DVT, n = 120). Through qRT-PCR, the expression of miR-106a-5p in serum was assessed, and its diagnostic value and disease correlation were evaluated through ROC curve, correlation analysis, and logistic analysis. In the study of cellular functions in vitro, oxidized low-density lipoprotein (ox-LDL) was used to induce human umbilical vein endothelial cells (HUVECs) and regulate miR-106a-5p. The targeting relationship of miR-106a-5p with TXNIP was authenticated via luciferase reporter experiments. This study found that miR-106a-5p was significantly decreased in the serum of burn patients with DVT, and it holds a high diagnostic value for DVT, suggesting its possible role as an independent factor associated with the occurrence of DVT in burn patients. In the in vitro experiment, the ox-LDL-induced HUVECs cell model demonstrated that miR-106a-5p overexpression promotes cell viability and suppresses the secretion of proinflammatory cytokines (TNF-α, IL-6, and IL-1β). In addition, TXNIP was significantly elevated in the DVT group and showed a significant negative correlation with miR-106a-5p. In summary, miR-106a-5p is downregulated in burn patients with DVT and holds promise as an early diagnostic marker. miR-106a-5p inhibits the inflammatory response by targeting TXNIP, and this finding provides an important basis for subsequent related research and clinical applications.