MicroRNA-2110 acts as a tumor suppressor in gastric cancer via direct targeting of IGF1R

Abstract

Abstract Background MicroRNA-2110 has been implicated in cancer, but its expression and function in gastric cancer (GC) remain understudied. This study aims to explore the role of miR-2110 in GC and its regulation of IGF1R. Methods RT-qPCR was used to measure miR-2110, IGF1R expression in 138 paired GC and adjacent normal tissues. Expression levels were also assessed in the normal gastric epithelial cell line GES-1 and three GC cell lines. Cell proliferation was evaluated by CCK-8 assays. Cell migration and invasion were determined using Transwell assays. The targeting relationship between miR-2110 and IGF1R was validated by dual-luciferase reporter assays. Furthermore, rescue experiments were performed to assess whether IGF1R overexpression reversed the effects of miR-2110. Results MiR-2110 expression was significantly lower in GC tissues and cell lines compared to normal controls, whereas IGF1R expression was significantly elevated. Reduced miR-2110 expression was significantly associated with tumor stage, metastasis, and poorer overall survival. Dual luciferase reporter assays confirmed IGF1R as a direct target of miR-2110. Overexpression of miR-2110 suppressed GC cell proliferation, migration, and invasion, whereas simultaneous overexpression of IGF1R partially reversed these inhibitory effects. Conclusions MiR-2110 suppresses malignant behavior of GC cells by targeting IGF1R.