Chinese Academy of Agricultural Sciences

A novel Alhagi honey polysaccharide-Alum Pickering emulsion adjuvant enhances systemic and mucosal immunogenicity of a BVDV vaccine in calves

Mucosal surfaces are the primary barrier to infection. For pathogens like bovine viral diarrhea virus (BVDV) that invade via the intestinal mucosa, vaccines capable of inducing mucosal immunity are crucial for mounting a comprehensive immune response. In this study, a new assembl...

The Uncertainty of Home Country’s Monetary Policy and Chinese Outward Foreign Direct Investment

Home-country monetary policy uncertainty affects outward foreign direct investment (OFDI). Taking A-share nonfinancial listed companies in China from 2004 to 2014 as the sample, this article explores the influence of home-country monetary policy uncertainty on OFDI. The results s...

Natural variation in GmRVE4d facilitates soybean latitudinal adaptation by regulating GmPRR5a-dependent flowering and maturity

Abstract Timely flowering and maturity are crucial for plant reproduction and environmental adaptation. As a core molecular system for plants to perceive environmental cues, the circadian clock integrates cyclic photothermal signals with intrinsic developmental programs, exerting...

Calcium: A Pivotal Regulator of Panicle Apical Abortion in Rice

Panicle size is a critical determinant of grain yield in rice and other cereal crops. During rice growth and development, panicle apical abortion (PAA) frequently occurs, particularly under unfavorable conditions, resulting in a reduced number of fertile spikelets and substantial...

A Two-Step Hybrid Statistical and Machine-Learning Framework with Full Genetic Effects for Multi-Environment Genomic Prediction in Maize

Accurate genomic prediction across environments remains challenging because phenotypic variation is jointly influenced by environmental conditions, genetic effects, and genotype-by-environment interactions. We developed a two-step hybrid statistical and machine-learning framework...

Solanaceous-Vegetable Price Volatility and Producer Operating Income in China: Evidence from Three Production-Side Response Indicators

Solanaceous vegetables are fresh and perishable, are marketed in concentrated periods, and require production decisions to be made in advance; consequently, fluctuations in the price path can alter producers’ cash-flow arrangements, input schedules, and risk exposure. Using a bal...

An enhanced model for accurate fish keypoint detection and tracking in intensive aquaculture

Monitoring fish growth is critical for intensive aquaculture, yet traditional manual weighing methods are labor-intensive and inefficient. This study proposes FishPose-tracking, an enhanced deep learning model that automates keypoint detection and tracking of fish, improving the...

Multi-layer Cis -molQTLs Reveal Regulatory Architecture and Enhance Heritability Explanation for Complex Traits in Cattle

Abstract Genome-wide association study (GWAS) analyses have identified numerous loci associated with economic traits in cattle. Many of these loci reside in noncoding regions, and the regulatory mechanisms through which they influence complex traits remain poorly understood. Here...

Preventive Effects of Fermented Yak Milk-Derived Lacticaseibacillus paracasei CD12-1 Against DSS-Induced Colitis in Mice

Ulcerative colitis is a chronic inflammatory bowel disease characterized by mucosal barrier disruption, dysregulated immune responses, and gut microbial imbalance. However, limitations of current therapies highlight the need for safe probiotic interventions. This study evaluated...

Evaluation of An I226R and A137R deletion mutant from a genotype I/II recombinant African swine fever virus strain as a live attenuated vaccine in pigs

Abstract Background: The emergence of highly lethal genotype I/II recombinant African swine fever virus (ASFV) strains in China has rendered existing genotype II-based live attenuated vaccines ineffective, underscoring the urgent need for novel vaccine candidates. Although single...

Functional Analysis of GhRNG1L Reveals Its Positive Role in Drought Tolerance Mediated by GhDREB2B in Cotton

Drought stress is a major abiotic factor limiting cotton yield and quality. RING-type E3 ubiquitin ligases play central regulatory roles in plant stress responses; however, the functions and regulatory networks of this gene family in cotton drought response remain largely unclear...

Integrated Adaptive Responses of Rhizosphere Microbial Communities and Functional Genes to Cadmium Stress in Ryegrass (Lolium perenne L.)

Heavy metal pollution impacts on soil microorganisms are of growing concern. This study employed high-throughput sequencing and qPCR to investigate rhizosphere microbial communities and functional genes responses in ryegrass (Lolium perenne L.) under cadmium (Cd) stress. Results...

Mitigation of ammonia and nitrous oxide emissions and cutdown of composting cycle during Enteromorpha prolifera composting with the co-addition of attapulgite and biochar

Abstract Since 2007, annual blooms of the green macroalga Enteromorpha prolifera have occurred in China’s coastal waters, posing serious threats to local aquaculture and ecological environment. Composting is an ideal way for managing E. prolifera waste. This study explored how at...

Dynamic Evolution of Volatile Aroma Compounds in Black Ginseng During Nine-Steaming and Nine-Drying Based on HS-GC-IMS and Chemometrics

Headspace–gas chromatography–ion mobility spectrometry (HS-GC-IMS) combined with chemometrics was employed to track volatile aroma compounds during the nine-steaming and nine-drying process. A total of 84 volatile signals (including monomers, dimers, and polymers) were tentativel...

Divergent Proton-Buffering Processes and Acidification Risks in Permanent and Variable-Charge Soils

Soil acidification threatens agroecosystems, yet the coupled, soil-specific proton-buffering mechanisms in permanent-charge soils (PCSs) and variable-charge soils (VCSs) remain insufficiently quantified. This study systematically investigated surface cation exchange, vacant site...

Genome-wide association study of the cecal microbiota revealing novel genetic insights into lipid deposition

Abstract Background Lipid deposition is a critical indicator of the economic and nutritional value of animal products. Among various regulatory factors, the cecal microbiota has been recognized as an important effect influencing lipid deposition capacity in tissues such as skin f...

Expression and immunoassay application of full-length recombinant antibody recognizing chlorantraniliprole using Pichia pastoris expression system

Abstract Full-length recombinant antibodies (rAbs) expressed in yeast offer the advantage of a eukaryotic system ensuring proper folding and bioactivity, along with high yield and low cost. In this study, plasmids encoding the light chain and heavy chain of the anti-chlorantranil...

Development and Validation of an Antigen-Capture ELISA for Quantitative Detection of Donkey-Origin Rotavirus: Application in Vaccine Manufacturing Process Monitoring

Rotavirus A (RVA) causes severe diarrhea in donkey foals, but rapid quantitative antigen assays for vaccine development and manufacturing control are lacking. We developed a VP6-based antigen-capture ELISA (acELISA) for donkey-origin RVA using rabbit anti-RVA polyclonal IgG as th...

Genome-wide identification and expression pattern analysis of the Medicago sativa 4-coumarate: CoA ligase (4CL) gene family

4-Coumarate: CoA ligase (4CL) is a pivotal enzyme in the phenylpropanoid metabolic pathway and plays a crucial role in plant growth, development, and stress responses. In this study, a genome-wide analysis was conducted to identify a total of 43 Ms4CL genes ( Ms4CLs ) in ‘Xinjian...

Prolonged Sublethal Imidacloprid Exposure Reduces the Functional Response and Searching Efficiency of Orius similis Against Frankliniella occidentalis

ABSTRACT Neonicotinoid residues can compromise biological control in integrated pest management, yet the role of exposure duration is rarely quantified for predatory bugs. Here, we compared short‐term (24 h) and long‐term sublethal exposure to imidacloprid (LC 10 and LC 20 ) on t...