The review examined research on roads built for highways, logging, plantations and unmapped access in tropical forests. Forest loss was greater near roads, in areas with more or longer roads, and after road construction or completion; one study in New Guinea found loss extending 17.7 kilometres from secondary roads.

Roads also created shorter-range ecological effects. Structural changes at forest edges reached 34 metres at a logging road and 350 metres at a highway. Reptiles and amphibians were the most common roadkill group in seven surveys covering all vertebrate classes, while paved roads reduced crossings by elephants and gibbons and were linked to genetic differences in some animals.