Enteric pathogens in soil adjacent to public waste bins in Maputo, Mozambique offers potential for environmental surveillance

Abstract

Abstract Wastewater surveillance has been widely adopted since the COVID-19 pandemic, but non-sewered or onsite sanitation is a common form of sanitation in cities of low- and middle-income countries. Environmental surveillance in these settings requires expanding analyses beyond wastewater. We collected 81 soil samples adjacent to public waste bins inside the sewered and non-sewered areas of Maputo and a 150-meter-wide buffer zone between the two areas, as well as from subsistence farms near the wastewater treatment plant for comparison. We cultured Escherichia coli ( E. coli) using the IDEXX Quanti-Tray/2000 system and determined the prevalence of 29 unique enteric pathogens via RT-qPCR on TaqMan array cards. E. coli concentrations were significantly higher (p<.001) in soils adjacent to public waste bins (mean = 5.05×10 5 per gram) compared to soils from farms (mean = 8.70×10 1 per gram). The mean number of unique pathogens was higher in soils from the non-sewered area (mean = 7.9, n=32) and the 150-meter buffer area (mean = 10.5, n=10) compared to the sewered area (mean = 4.6, n=20) and soils from farms (mean=3.8, n=19). Findings demonstrate that the presence of enteric pathogens in soils adjacent to public waste bins were associated with neighborhood sanitation infrastructure and may be a useful matrix for surveillance. In high-burden settings with poor sanitation, direct examination of soils and other environmental matrices are potentially scalable means of environmental pathogen surveillance to consider beyond conventional sampling matrices.