Development and validation of a real-time polymerase chain reaction for the detection of Haycocknema perplexum.
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Abstract
dilution (Ct value 39.6) for SSU-PCR, making the latter a more sensitive assay for detecting lower concentrations of organism in the patient biopsy. The sensitivity of fresh frozen samples was superior to FFPE samples. All but one sample was negative following treatment. Feasibility of real-time PCR detection of H. perplexum directly from tissue biopsies has been demonstrated for diagnosis, possible test of cure, and could enhance transmission surveillance.
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Authors: Kym Lowry, Bijendra Parmar, Rita Gupta, T. Robertson, David Whiley, Patrick N A Harris, Natalie Davidson
Institutions: The University of Queensland, Queensland Health, QIMR Berghofer Medical Research Institute