University Hospital Carl Gustav Carus

Next Generation Sequencing for the Differentiation of Benign and Malignant Biliary Strictures

The differentiation between benign and malignant biliary strictures remains a significant clinical challenge. Recent studies have suggested that next generation sequencing (NGS) can improve the diagnostic accuracy. However, evidence on its performance in routine clinical practice...

Beyond in silico prediction: multi-omics to identify a pathogenic deep intronic HNRNPK variant in Au-Kline syndrome

Abstract Pathogenic variants in HNRNPK are associated with autosomal dominant Au-Kline syndrome (AKS, Au-Kline-Okamoto syndrome, OMIM #616580). This syndrome is characterized by developmental delay and intellectual disability, hypotonia, and distinctive facial features. Despite t...

Functional characterization of snoRNA-derived RNA (sdRNA) expression in healthy hematopoiesis and acute myeloid leukemia

Abstract SnoRNAs are highly expressed in AML and have implications in leukemogenesis and leukemic maintenance. SnoRNAs can be further processed into snoRNA-derived RNAs (sdRNAs). The role of sdRNAs in AML and healthy hematopoiesis remains largely elusive. We characterized sdRNA a...

Olfactory Training and Depressive Symptoms in Older Adults: A Randomized Placebo‐Controlled Trial With Exploratory Evidence in Moderate Depression

ABSTRACT The risk of developing depressive symptoms increases with age. The following study aimed to verify if olfactory training may relieve symptoms of depression in the older population. Older participants ( N = 101, M age = 72.6, SD = 5.44, 88% women) participated in this stu...

Systemic Metabolic Changes in Plasma of Patients with Myelodysplastic Neoplasms and Chronic Myelomonocytic Leukemia

Background: Myelodysplastic neoplasms (MDSs) are clonal hematopoietic stem cell disorders associated with ineffective hematopoiesis, chronic inflammation, and increased cardiovascular morbidity. Although metabolic dysregulation has been implicated in MDS pathogenesis, systemic me...

Nationwide trends in incidence and in-hospital mortality of ARDS in Germany, 2008–2022

Abstract Acute respiratory distress syndrome (ARDS) remains a major contributor to morbidity and mortality among critically ill patients. Recent events such as the COVID-19 pandemic and demographic changes have likely influenced its epidemiology. This study aimed to investigate n...

Twelve-Month Outcomes After Hearing Rehabilitation With an Active Middle Ear Implant and Latest Generation Vibroplasty Couplers in Adults And Children: A Prospective Multicenter Study

OBJECTIVE: To evaluate the audiological efficacy, patient-reported outcomes, and safety of an active middle ear implant (AMEI) in children and adults with distinct coupling options. STUDY DESIGN: Prospective, longitudinal, multicenter, noninterventional observational study with r...

Machine learning provides individualized prediction of outcomes after first complete remission without allo‐HSCT consolidation in adult acute myeloid leukemia—A HARMONY study

Abstract Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a curative treatment option for a significant proportion of patients with acute myeloid leukemia (AML), and it is generally recommended when the relapse risk without allo‐HSCT outweighs the estimated non‐r...

Pediatric intensive care follow-up in Germany is rarely established and highly heterogeneous: a nationwide survey of 20 PICUs

BACKGROUND: Survival after pediatric critical illness has improved substantially, but many survivors and their families experience long-term physical, neurocognitive, psychological and social sequelae, summarized as post-intensive care syndrome in pediatrics and in families. Stru...

Variation in meningioma recurrence risk estimates across observational cohorts: the influence of calendar time, WHO classifications, geographical settings, and healthcare systems

Abstract Purpose Recurrence risk estimates underpin meningioma research, including molecular classification and clinical trial benchmarking, yet are often based on retrospective or historical data. The aim of this study was to assess the variation of recurrence risk estimates acr...

Correlation of [ 68 Ga]Ga-RAYZ-8009 PET/CT Uptake with Immunohistochemical Glypican-3 Expression in Hepatocellular Carcinoma: A Proof-of-Target Study

Glypican-3, a cell-surface proteoglycan, is overexpressed in most hepatocellular carcinomas (HCCs). Recently, the glypican-3–targeted PET tracer [68Ga]Ga-RAYZ-8009 was shown to enable noninvasive detection of HCC. This retrospective analysis evaluates the correlation between tumo...

Tumor Necrosis is Associated with an Increased Metastatic and Cardiovascular Risk in Paragangliomas: A Single-Center Series and Meta-Analysis Comparing Necrosis and Cystic Degeneration in Paragangliomas

Abstract Paragangliomas (PGLs) are rare neural crest–derived tumors with highly variable morphology and clinical behavior. These tumors can present with cystic and necrotic alterations that may influence the clinical presentation of affected patients. However, prevalence and clin...

Selective Cell Death of Adrenocortical Cells by Pharmacological Activation of Mutant KCNJ5

BACKGROUND: Primary aldosteronism is frequently caused by mutations in the KCNJ5 (potassium inwardly rectifying channel subfamily J member 5) gene, encoding a G-protein inwardly rectifying potassium channel. These mutations disrupt the channel’s selectivity filter, permitting abn...

Mucus transport in the olfactory cleft is significantly slower than in the nasal respiratory mucosa.

Intranasal mucociliary transport has been conventionally described as moving, essentially, in an anterior-to-posterior direction along the ciliated respiratory mucosa (RM). This mechanism is essential for clearing inhaled particles and pathogens and may be impaired in individuals...

External validation of a top-ranked model from the RSNA pulmonary embolism detection challenge: assessment of generalizability

Abstract To externally validate the RSNA 2020 challenge 2 nd -place deep-learning (DL) algorithm for detecting pulmonary embolism (PE) on computed tomography pulmonary angiography (CTPA) scans with additional focus on subsegmental-only PEs (SSPE). Between 2015 and 2022 1,038 CTPA...

Quantifying the Interplay Between PRAME Expression and Classical Morphology: A Multivariable Analysis of 954 Suspected Melanocytic Lesions

The integration of immunohistochemical biomarkers like PRAME (Preferentially Expressed Antigen in Melanoma) into the histomorphological assessment of melanocytic lesions is gaining prominence. While PRAME’s diagnostic value is widely recognized, its independent weight compared di...

Uncovering the scale and link of two SARS-CoV-2 outbreaks in nursing homes by combining integrated genomic surveillance, contact tracing, and point-of-care testing

Abstract Aim The local public health authority (LPHA) in Duesseldorf received notification of two SARS-CoV-2 outbreaks in nursing home A and nursing home B in October 2021 and embarked on an outbreak investitation to identify the source of the outbreak and epidemiological links b...