The Seventh Affiliated Hospital of Sun Yat-sen University

Conformationally Restricted GalN3 Donors Enable Zinc(II)-Mediated Stereocontrolled Galactosylation

Abstract We report a Zn(II)-mediated stereocontrolled galactosylation with two conformationally restricted GalN3 donors that enables divergent α- and β-glycosylation. The 4,6-O-TIPDS/3-O-Bz donor selectively affords α-GalN3 glycosides, while the 4,6-O-benzylidene/3-O-TIPS donor p...

mRNA-LNP vaccines targeting conserved virulence factors elicit protective immunity against lethal Legionella pneumophila infection

Legionnaires’ disease (LD), caused by the intracellular pathogen Legionella pneumophila , imposes a substantial global health burden, yet no vaccine has been clinically approved. Here, we develop mRNA-LNP vaccines encoding conserved virulence factors Pal, PilE, and FlaA. In BALB/...

Haploidentical hematopoietic stem cell transplantation compared to immunosuppressive therapy plus eltrombopag as first-line treatment for severe aplastic anemia patients aged 40–65: a multicenter retrospective study

Abstract Immunosuppressive therapy (IST) plus eltrombopag (EPAG) (IST+EPAG) is the preferred first-line treatment for severe aplastic anemia (SAA) patients over 40 years. However, direct comparison between IST+EPAG and upfront haploidentical hematopoietic stem cell transplantatio...

Bone‐Targeted Engineered Exosomes Delivering Betaine Alleviate Osteoporosis via Autophagy‐Driven Osteogenesis

Dysregulated bone remodeling, attenuated endogenous osteogenic capacity, and the lack of bone-targeting capability in therapeutics constitute the core challenges in current clinical interventions for osteoporosis. In this study, a hybrid nanodelivery system integrating betaine-lo...

Phenothiazine-Driven Autophagic Response in Macrophages as an Anti-cutaneous Tuberculosis Approach

Abstract Cutaneous tuberculosis (CTB), caused by Mycobacterium tuberculosis (Mtb) infection of the skin, remains a global health burden, underscoring the urgent need for more effective drugs and therapeutic strategies. In this study, we discovered a phenothiazine-based derivative...

Clinical and molecular epidemiology of type 2 diabetes mellitus-associated intestinal Staphylococcus aureus colonization

ABSTRACT Intestinal Staphylococcus aureus colonization in type 2 diabetes mellitus (T2DM) remains poorly defined with respect to prevalence, clinical associations, and molecular characteristics. We investigated intestinal S. aureus colonization in 142 patients with T2DM and chara...

Genomic divergence and phenotypic heterogeneity of Legionella longbeachae reveal a putative novel proinflammatory serogroup with genome reduction and mobilome expansion

ABSTRACT Legionella longbeachae is an emerging respiratory pathogen primarily found in soil environments, yet its genomic architecture and evolutionary strategies remain poorly characterized. Here, through pan-genomic and functional analysis of 242 L. longbeachae isolates, we rev...

Integrative phylogenomics, polyphasic taxonomy, and metagenomics expand the characterized taxonomic diversity of Thermoleophilia

Abstract The class Thermoleophilia represents a deep‐branching lineage within the phylum Actinomycetota , and yet, its taxonomic diversity remains incompletely resolved due to the limited availability of cultured representatives and uneven integration of genome‐resolved diversity...

Parenting styles and fertility intention: Mediating effects of childbearing beliefs and psychological resilience

China's declining fertility rate is partly due to low fertility intention among childbearing population. To understand family influence on young people's fertility intention and its underlying mechanisms, this study examines how parenting styles (authoritarian vs. permissive) aff...

Short-term neonatal in-hospital associations following delivery room ETI versus PPV resuscitation: a multicenter retrospective cohort study

Abstract Background Endotracheal intubation (ETI) and positive pressure ventilation (PPV) are core neonatal resuscitation interventions. Baseline clinical imbalances confound gestational-age (GA)-specific associations between ETI and in-hospital adverse events. We evaluated GA-st...

Safety and satisfaction of voluntary medical male circumcision among men who have sex with men in China

PURPOSE: To investigate the safety and satisfaction of circumcision among men who have sex with men (MSM), as voluntary male medical circumcision (VMMC) may be an innovative and acceptable tool for HIV prevention among this population in the future. METHODS: Participants who volu...

UA-30 ameliorates motor deficits through RalA-mediated mitophagy in ALS mice

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive motor neuron loss, skeletal muscle atrophy, paralysis, and eventually death. Mitochondrial dysfunction plays a pivotal role in ALS pathogenesis, although the precise pathogenic mechani...

The impact for causal associations between common diseases and inflammatory bowel disease: a disease-wide bidirectional Mendelian randomization study

Abstract Objectives Observational studies on associations between various diseases and inflammatory bowel disease (IBD) are often limited by confounding and reverse causation. We aimed to assess potential causal relationships between a wide range of diseases and IBD, including Cr...

Evaluative performance of triglyceride-glucose-frailty index versus surrogate insulin resistance indices for advanced cardiovascular-kidney-metabolic syndrome, cardiometabolic multimorbidity, and mortality: the role of estimated glomerular filtration rate

Cardiovascular-kidney-metabolic (CKM) syndrome and cardiometabolic multimorbidity (CMM) carry substantial mortality, yet whether a composite index integrating insulin resistance and frailty shows stronger associations with advanced CKM syndrome, CMM, and mortality than convention...

Clinical outcomes and molecular features of CEBPA-mutated MPAL: a multi-center case series

Mixed phenotype acute leukemia (MPAL) is rare and aggressive, with no standard therapy. CEBPA mutations define favorable-risk AML, but their significance in MPAL is unknown. We reviewed eight CEBPA -mutated MPAL patients from five Chinese centers. Four received allo-HSCT in first...

Longitudinal plasma phosphorylated tau217 classification and the association with Alzheimer's disease progression

Abstract INTRODUCTION Longitudinal diagnostic and prognostic validity of plasma phosphorylated tau217 (p‐tau217) in Alzheimer's disease (AD) remains uncertain. METHODS In this multi‐cohort study of 2117 individuals, we established baseline plasma p‐tau217 thresholds for amyloid‐β...

Genomic predisposition is associated with the direction of sex chromosome evolution

Sex chromosome evolution is among the most dynamic genomic innovations in vertebrates, yet why some lineages evolve XY while others evolve ZW remains unclear. Using chromosome-level genomes from 19 geckos, we found that gecko sex chromosomes originated independently from 16 ances...

Drosha in mesangial cells regulates glomerular capillary tufts formation through drosha/ribosome/Gata3 axis

Abstract Drosha is a critical regulator of kidney development; its loss or mutation, identified in a subsets of Wilms tumor (WT), a pediatric kidney cancer, leads to nephric cap mesenchyme developmental defects. Here, we investigated the role of Drosha in mesangial cells and its...

YAP delactylation enhances therapy resistance in renal cell carcinoma

Abstract Lactylation is a novel lysine acylation modification, and its function and exact mechanisms remain unclear. The responsiveness to combined targeted and immunotherapy continues to be a major challenge in cancer treatment. Here, we discovered that lysine lactylation (Kla)...

A Heating-AssistedIntegrated Purification-Enrichment-DerivatizationPaper Chip for Rapid MS/MS Quantification of Serum Steroid Hormones

Abstract Rapid and reliable simultaneous quantification of multiple steroid hormones is important for the diagnosis and monitoring of endocrine disorders such as polyendocrine metabolic ovarian syndrome (PMOS), formerly known as polycystic ovary syndrome (PCOS). Existing methods...