Science for Life Laboratory

Plasma membrane order maps functional diversity in immune cells

Abstract Cell membranes undergo biophysical remodeling as an adaptation to the surroundings and to perform specific biological functions. However, the extent and relevance of such changes in human immune cells remain unknown, largely because of the lack of single-cell and multidi...

Immune-checkpoint inhibitor-induced bullous pemphigoid in patients with metastatic renal cell carcinoma: two case reports and systematic review of the literature

BACKGROUND AND PURPOSE: Immune-checkpoint inhibitors (ICIs) improve outcomes in renal cell carcinoma (RCC) but may cause immune-related adverse events (irAEs), including cutaneous toxicity. Bullous pemphigoid (BP) is a rare but clinically significant irAE that can require ICI dis...

Impact of Single‐Cell RNA Reference Selection for the Deconvolution of Breast Cancer Spatial Transcriptomics Datasets

Spot-based spatial transcriptomics (ST) allows for unbiased gene expression analysis within tissue architecture, overcoming the limitations of single-cell RNA sequencing (scRNA-seq) by preserving spatial context. However, the high spatial resolution in ST leads to cellular hetero...

Interpreter use in Nordic paediatric oncology care – a multicentre cross-sectional study

BACKGROUND AND PURPOSE: Tailored, language-appropriate information is fundamental for children and their families in paediatric oncology, influencing both the quality of care and clinical outcomes. Yet, for families who speak another language, interpreter services are not used to...

Experimental radiotherapy with the gastrin-releasing peptide receptor (GRPR) antagonist [177Lu]Lu-AU-SAR-M1 in a prostate cancer murine model using reduced-frequency dosing

The gastrin-releasing peptide receptor (GRPR) is highly expressed in prostate and breast cancers and hence serves as a legitimate target for radionuclide therapy (TRT). Radiolabeled GRPR antagonists are attractive radionuclide-carriers for TRT, due to their rapid tissue penetrati...

Ultra‐Tough Hybrid Supramolecular Gelatin Hydrogels

ABSTRACT Natural load‐bearing tissues derive mechanical performance from sequential self‐assembly, in which collagen fibrils first compact and are then stabilized by enzymatic covalent crosslinking. Replicating this design in synthetic hydrogels while limiting swelling and preser...

Genomic characterization of ER-positive primary tumors and corresponding relapses identifies potentially targetable alterations

The majority of breast cancer patients have tumors expressing estrogen receptor α (ER) and receive endocrine therapy. However, around one-third relapse in their disease, predominantly with retained ER expression. Molecular alterations are proposed to be contributors to the resist...

Early definitive fracture fixation (EDFF) does not alter circulatory neutrophil activation status in physiologically restored polytrauma: a 72-h porcine observation study

Abstract Dysregulation of the cellular innate immune system, particularly involving leukocytes and mainly neutrophils, underlies post-traumatic inflammatory complications. Early definitive fracture fixation (EDFF) triggers immune activation and may exacerbate inflammatory complic...

Structure-guided allosteric modulation of the delta opioid receptor

Abstract Opioid analgesics remain essential for pain management but are associated with significant adverse effects, including respiratory depression, tolerance, and dependence. The δ-opioid receptor (δOR) represents a promising therapeutic target for developing safer opioid anal...

RNA degradation by DIS3 is a necessary step in the resolution of backtracked transcription complexes

Summary The RNA exosome is known to participate in transcription, but the contribution of its ribonuclease activities to this process remains unclear. Here we investigated the role of DIS3, one of the exosome ribonucleases, in transcription by RNA polymerase II (RNAPII). Rapid de...

High-Ratio Partial Proteolysis with Carrier Proteome (HOLSER) Enables Global Structure Profiling and Site-Resolved Elucidation of Ligand-Protein Interactions.

Understanding how cellular proteins interact with their environment, including endogenous and exogenous molecules, is critical for elucidating mechanisms of cellular regulation and drug action. Partial proteolysis-based techniques offer peptide-level resolution of ligand-induced...

Multi-omics profiling reveals gene signatures and therapeutic targets in HER2-guided gastric cardia adenocarcinoma patients

Abstract Therapeutic targets for gastric cardia adenocarcinoma (GCA), particularly for HER2-negative patients, are lacking. Here, we conducted multi-omics profiling on 128 GCA patients using mass spectrometry, whole-exome sequencing, RNA-Seq, scRNA-Seq and spatial transcriptomics...

Cell surface remodeling caused by the loss of the flippase subunit TMEM30A in immune cells

Plasma membrane lipid asymmetry is tightly regulated and fundamental to mammalian cell physiology. TMEM30A is the β-subunit of P4-ATPases, flippase enzymes that maintain strict phosphatidylserine (PS) asymmetry by pumping it from the outer to the cytosolic leaflet. Loss of TMEM30...

Genome-wide association study of image-based emphysema scoring in the Swedish CArdioPulmonary bioImage Study (SCAPIS) suggests two new risk loci in smokers

Abstract Background Despite the high prevalence and clinical significance of emphysema, few genetic risk loci have been consistently replicated. We conducted a genome-wide association study (GWAS) of CT-based emphysema, with a particular focus on non-smoking-related genetic deter...