Nanjing Drum Tower Hospital

Metabolically unhealthy obesity is a novel risk factor for cisplatin- associated nephrotoxicity in patients with bladder cancer: a retrospective cohort study

Abstract Purpose Cisplatin-associated nephrotoxicity limits chemotherapy in bladder cancer patients. Obesity defined by body mass index (BMI) has yielded conflicting findings regarding the prediction of nephrotoxicity, possibly due to metabolic heterogeneity within the obese popu...

Mppa sustains cyst stem cell maintenance and orchestrates germ cell differentiation through nonautonomous regulation of mitochondrial homeostasis in Drosophila testes

Abstract The intricate balance maintaining CySCs and facilitating their differentiation within the testicular stem cell niche remains a complex process. However, the mechanisms underlying CySC maintenance and their nonautonomous impact on GSC differentiation and downstream effect...

Triple network effective connectivity imbalance across the Alzheimer’s disease spectrum and its modulation by intermittent theta burst stimulation

Alzheimer’s disease (AD) is characterized by progressive cognitive decline, yet the directed network mechanisms underlying this process and their responsiveness to neuromodulation remain incompletely understood. Guided by the triple network model, we investigated effective connec...

The mechanosensitive channel TMEM63B contributes to noxious heat sensation in the dorsal root ganglia

In mammals, heat sensation is primarily mediated by thermosensitive channels, particularly transient receptor potential (TRP) channels, in dorsal root ganglion (DRG) neurons. However, whether additional channels contribute to noxious heat detection remains unclear. Here, we inves...

Sex-Specific Plasma Proteomic Alterations Underpin Distinct COVID-19 Progression and Post-COVID-19 Condition

Abstract Sex differences in COVID-19 and its sequelae remain poorly understood, which hinders targeted therapies. We performed plasma proteomic analyses of 69 hospitalized patients with COVID-19 and 30 healthy controls from a prospective Chinese cohort. The UK Biobank and Mount S...

Efficacy and Safety of Modified Double-snare-assisted Endoscopic Mucosal Resection for Small Rectal Neuroendocrine Tumours: A Preliminary Study

Abstract Background Modified double-snare-assisted endoscopic mucosal resection (mDS-EMR) through single-channel endoscopy has been reported as a promising technique for treating rectal neuroendocrine tumours (NETs). We evaluated the efficacy and safety of mDS-EMR for small recta...

A Single-Cell Transcriptomic Map Reveals Platelet-Rich Plasma-Induced Endometrial Microenvironment Remodeling in Recurrent Implantation Failure

Abstract Intrauterine infusion of platelet-rich plasma shows clinical promise as a regenerative therapy for patients with repeated implantation failure, yet the precise cellular and molecular mechanisms governing its in vivo effects on the human endometrium remain poorly understo...

Integrative Single-Cell Proteomics and Network Analysis Identifies DHX9 as a Key Regulator of Oocyte Maturation and a Therapeutic Target for Oocyte Developmental Arrest

Abstract Oocyte developmental arrest at the germinal vesicle (GV) or metaphase I (MI) stage is a major cause of infertility, yet its molecular mechanisms remain poorly understood. Here, we performed ultradeep single-cell proteomics on 8 human oocytes (4 GV-stage and 4 MI-stage) a...

Role of desolvation on biomolecular liquid–liquid phase separation

Abstract Biomolecular condensates play essential roles in cellular organization and are implicated in diverse pathological processes. Their formation is driven by liquid-liquid phase separation (LLPS), a process that requires coordinated multistep desolvation of biomolecular chai...

Preclinical Evaluation of 68Ga-Labeled Heterodimers for Dual Integrin αvβ3 and PARP1-Targeted Tumor Imaging

Abstract Tumor heterogeneity limits the diagnostic performance of molecular imaging probes targeting a single biomarker. Here, we report the design, synthesis, and preclinical evaluation of 68Ga-labelled heterodimeric radiotracers co-targeting integrin αvβ3 and poly(ADP-ribose) p...

The total serum N-glycome landscape of hematological malignancies

A holistic and dynamic view of the glycomic landscape in hematological malignancies (HMs) remains poorly characterized, hindering clinical the application of glycomics in HMs. We launched a large-scale analysis of multicenter cohorts, combining retrospective (n=1,370) and prospec...

PTTG1 drives intestinal-type gastric cancer progression via G2/M checkpoint-mediated cell-cycle reprogramming

Intestinal-type gastric cancer (IGC) progresses through the Correa cascade, yet the molecular determinants of this transition remain insufficiently defined. Using single-cell RNA sequencing (scRNA-seq) on matched normal, intestinal metaplasia, and tumor tissues from five patients...

Gender differences in the association between metabolic syndrome components and 5-year risk of hyperuricemia: a retrospective cohort study

Hyperuricemia is a recognized risk factor for cardiovascular disease and is closely linked to metabolic syndrome (MetS). This study aimed to assess the impact of MetS and its components on the 5-year risk of developing hyperuricemia, specifically focusing on gender differences. T...

Harnessing cell cycle synchronization to boost the therapeutic potential of umbilical cord MSCs against acute colitis

Abstract Human umbilical cord mesenchymal stromal cells (UCMSCs) are multipotent stem cells with strong immunomodulatory capacity, ease of acquisition, low immunogenicity, and a non-tumorigenic nature, making them attractive candidates for tissue repair. Under standard culture co...

Pola-R-CHP in previously untreated DLBCL with international prognostic index score 0–1: a multicenter real-world retrospective study in China

Abstract Purpose Approximately 30% of newly diagnosed diffuse large B-cell lymphoma (DLBCL) cases are categorized as low-risk by the international prognostic index (IPI). Polatuzumab vedotin, rituximab, cyclophosphamide, doxorubicin, and prednisone (Pola-R-CHP) has demonstrated e...

Functional myocardial ischemia assessment based on HF-ECG microstructural features

Early detection and stratification of functional myocardial ischemia are critical for timely intervention but remain constrained by the limited sensitivity of conventional electrocardiography (ECG) and the cost and accessibility of imaging-based diagnostics. We present a non-inva...

Advancing Understanding of Clean Heating Benefits: Respiratory Symptom Evidence Among Rural Adults

Weikai Dong, M.D, Qifeng Song, M.D, Qiang Wang, M.D., Prof; Advancing Understanding of Clean Heating Benefits: Respiratory Symptom Evidence Among Rural Adu

Body mass index and semen quality in 11 154 men: evidence from a large-scale andrology dataset

Obesity has been increasingly linked to impaired male reproductive function, yet the association between body mass index (BMI) and semen quality remains unclear, particularly in large Asian populations. In this retrospective cross-sectional study, we analyzed 11 154 men aged 18–5...

Inhibition of neuronal DICER–miR-34a/346 signaling attenuates amyloid pathology in 5×FAD mice

MicroRNAs (miRNAs) are critical regulators of genes underlying Alzheimer’s disease (AD) pathogenesis, yet the role of neuronal DICER, a key ribonuclease essential for miRNA maturation, remains unclear. While biochemical analysis of human postmortem cortical tissues revealed upreg...

Comparative efficacy and safety of CD19 chimeric antigen receptor T-cell therapy versus CD3/CD20 bispecific antibodies in relapsed/refractory mantle cell lymphoma: a meta-analysis of clinical trials and real-world studies

CD19-directed chimeric antigen receptor T-cell (CAR-T) therapies and CD3/CD20-directed bispecific antibodies (BsAbs) showed high efficacy in relapsed/refractory mantle cell lymphoma, though comparative data are limited. We performed a meta-analysis of clinical trials and real-wor...