Nanjing Agricultural University

Global rice methane emissions tripled over six decades amid declining emission intensity

Abstract Rice paddies are among the largest anthropogenic sources of methane (CH4), yet substantial uncertainties persist in the long-term magnitude and spatiotemporal dynamics of the emissions. Here we show a spatially explicit global estimate of rice CH4 emissions from 1961 to...

Does the supply of eco-assets match the demand for eco-products spatially? A case study of the Yangtze River Delta urban agglomeration

The value realization of eco-assets through eco-product consumption requires both a sufficient ecological supply base and enabling demand-side conditions. Yet assessments in economically developed regions often focus on supply alone. A supply-only assessment can hide the spatial...

High endophytic fungal diversity and the antagonistic Aspergillus niger Bzrh13 are associated with Atractylodes macrocephala root rot resistance

Root rot severely impairs the growth of Atractylodes macrocephala , a valuable medicinal plant. Despite the recognized importance of endophytic fungi in plant health, the endophytic fungal community associated with A. macrocephala root rot remains poorly characterized. Combining...

Comparative genomics of the streptococcal mobilome reveals class-specific integration, mobility and antimicrobial resistance cargo

Abstract Mobile genetic elements (MGEs) drive genome plasticity, horizontal gene transfer, and antimicrobial resistance (AMR) dissemination in Streptococcus , yet genus-wide comparisons across major MGE classes remain limited. Here, we analyzed 1,961 complete genomes from 60 Stre...

Expression and immunoassay application of full-length recombinant antibody recognizing chlorantraniliprole using Pichia pastoris expression system

Abstract Full-length recombinant antibodies (rAbs) expressed in yeast offer the advantage of a eukaryotic system ensuring proper folding and bioactivity, along with high yield and low cost. In this study, plasmids encoding the light chain and heavy chain of the anti-chlorantranil...

Evaluating Spatial Distribution of Cultural Ecosystem Services (CESs) Based on Rural Landscape Characteristics

Cultural ecosystem service (CES) assessment is essential for comprehensively understanding and evaluating ecosystem services. Optimizing the spatial zoning of CESs can provide more targeted information for ecosystem management. By identifying the typical landscape characteristics...

A ticket to formal finance: credit rating and farmers’ financing choices

This paper examines how credit rating affects farmers’ financing choices within China’s dual rural financial structure. Using four waves of household survey data and a difference-in-differences model, we show that credit rating increases formal borrowing while reducing both inter...

Whole‐Genome Resequencing Reveals Selection Signatures Associated With Lambskin Patterns in Hu Sheep

ABSTRACT In China, Hu sheep lambskin quality is largely determined by wool curvature, with wavy‐wool lambs producing higher‐quality pelts than straight‐wool lambs. However, the genetic mechanisms underlying wool curvature formation remain poorly understood. To address this knowle...

Fragrant Rice Gains Enhanced 2-Acetyl-1-Pyrroline and Multi-stress Resistance Through a JA-Proline Module Induced by a Rhizopus Fungus

Abstract Rhizosphere microorganisms play crucial roles in regulating crop secondary metabolism and stress adaptation, yet the mechanisms by which they coordinately enhance both 2-acetyl-1-pyrroline (2-AP) and stress resistance in fragrant rice remain unclear. Here, we report a ne...

Superior Leaf Deposition and Subsequent Uptake: Nanocarrier Boosts Cyproflanilide for Enhanced Pest Control

Abstract Chemical pesticides remain indispensable for crop protection, but inefficient delivery and overapplication lead to resistance and environmental risks. Cyproflanilide is a novel meta-diamide insecticide active against a broad range of agricultural pests. Here, we develope...

ElectrochemiluminescenceMicroscopy

Abstract Electrochemiluminescence (ECL) microscopy is an emerging imaging technology that combines electrochemistry with optical microscopy. It operates without external light excitation, effectively eliminating photobleaching, phototoxicity, and scattered light noise, and thus e...

Variety-Seeking Behavior and Willingness to Pay for Staple Foods: Evidence from Online Food Delivery in China

Food security has always been the leading priority of national strategic deployment. Given the dual background of the continuous upgrading of dietary structures and the rapid growth of the food delivery industry, takeaway staple food waste has become increasingly prominent. Howev...

Integrated morphological, physiological, biochemical and transcriptomic analyses elucidate core pathways underlying genotypic differences in drought resistance of perennial grasses

Drought stress is the major abiotic factor limiting forage and turf grass growth and productivity. However, systematic studies on the response, adaptation, and molecular mechanisms underlying drought resistance in centipedegrass ( Eremochloa ophiuroides ) remain underexplored. He...

OsWOX13 functions as a heading signal transducer from integrator Ehd1 to florigen for the accurate regulation of rice heading date

Abstract Appropriate heading date is an important safeguard for stabilizing rice yield and quality. Heading integrator Ehd1 and florigen Hd3a are crucial for rice heading. However, the mechanism by which Ehd1 transmits heading signals to Hd3a and the accurate regulation of Hd3a e...

A Descriptive Analysis of Morbidity Patterns Among Insured Pet Cats in China Based on Insurance Claims

Population-level information on feline morbidity in China remains limited despite the rapid growth of the pet cat population. This study described morbidity patterns among insured cats in China using insurance claims from a major pet insurance provider, with diagnoses derived fro...

Machine Learning Guided Meta-transcriptomics Reveals OsRPM1 as a Key Regulator of Rice Blast Resistance

Rice blast, caused by Magnaporthe oryzae , is the most destructive fungal disease of rice, responsible for annual yield losses of 10–30% and posing a major threat to global food security. The rapid evolution of blast pathotypes and the polygenic nature of resistance complicate br...

Integrated Physiological and Multi-Omics Analysis Reveals Selenium-Mediated Drought Tolerance in Brassica napus Seedlings

Drought stress severely restricts early growth in Brassica napus, while selenium (Se) can enhance stress tolerance. This study examined how foliar sodium selenite pretreatment affects polyethylene glycol (PEG)-induced drought stress in B. napus seedlings. Seedlings were sprayed w...

Synergistic Effectsof Particle Size and MolecularWeight on Chain Entanglements in Single-Polymer Nanoparticles

Abstract The polymer dynamics at the nanoscale exhibit significant deviations from their bulk materials, affecting both our fundamental understanding and practical applications of polymers. Despite growing applications and synthesis advancements of 3-dimensional confined polymeri...

ProgrammableVersatile Socket: A Tight-Locking andHigh-Gain CRISPR/Cas12a Molecular Circuit for Ultrasensitive Sensingof Diverse Targets

Abstract CRISPR/Cas12a has emerged as an important platform for nucleic acid analysis, yet limited catalytic turnover and intrinsic nucleic acid specificity constrain its sensitivity and analyte scope. Herein, a tight-locking and high-gain Cas12a-driven strand displacement amplif...

Firmness Variation in Tomato Fruit: Driving Factors, Signaling Regulation, and Genetic Basis

Fruit firmness is a pivotal agronomic and commercial trait that determines the storability, transport tolerance and edible quality of tomato (Solanum lycopersicum L.). Fruit softening severely restricts postharvest performance and economic benefits of tomato products worldwide. T...