Garvan Institute of Medical Research

Detecting somatic mutations in rare clones using single-cell multiomics

Abstract Background Somatic mutations are increasingly recognised as drivers of diseases beyond cancer, including autoimmune disorders. However, identifying rare, cell type-specific causal mutations remains challenging due to their low frequency within heterogeneous cell populati...

Stochasticity in response to rostrocaudal and mediolateral determinants influences inaccurate specification within kidney organoids

The differentiation of human pluripotent stem cells to kidney requires the accurate recapitulation of complex morphogenetic patterning events within an in vitro environment. Current protocols are assumed to accurately pattern through intermediate mesoderm to kidney with outcomes...