First Affiliated Hospital of Kunming Medical University

Targeted RNA m5C demethylation of NFE2L2 mRNA by dCas13b-TET2 restores ferroptosis and overcomes cisplatin resistance in bladder cancer

Cisplatin-based chemotherapy remains a cornerstone for the treatment of advanced bladder cancer; however, the emergence of resistance severely limits its clinical efficacy. Ferroptosis suppression has been implicated in chemotherapy resistance, yet the upstream epitranscriptomic...

SIL1 modulates Reelin signaling during cortical neuron migration

Abstract SIL1 , a nucleotide exchange factor for the endoplasmic reticulum chaperone BiP, primarily binds nascent polypeptides to facilitate proper protein folding. It is a causative gene of Marinesco-Sjögren syndrome (MSS). Previous studies have indicated that SIL1 dysfunction d...

Data-driven clinical phenotypes of diabetic kidney disease and their association with disease progression: a real-world cohort study

Diabetic kidney disease (DKD) has a heterogeneous clinical course. A refined classification of DKD phenotypes could help identify patients at increased risk of kidney disease progression and guide individualized clinical management. This retrospective cohort included 6,667 adults...

Research progress on the diagnosis and treatment of far-out syndrome

Abstract Far-out syndrome (FOS) is a rare cause of L5 radiculopathy, resulting from extraforaminal compression of the L5 spinal nerve within the lumbosacral tunnel. The diagnosis requires the simultaneous fulfillment of two necessary conditions: compression located within this sp...

ANGPTL4 is a hypoxia associated candidate linked to immune and vascular phenotypes in ccRCC

Clear cell renal cell carcinoma (ccRCC) is characterized by prominent vascularization and cellular heterogeneity, but reliable biomarkers for angiogenesis and response to targeted therapy remain limited. In this study, transcriptomic data from GEO cohorts (GSE53757, GSE66271, GSE...

Stage-specific remodeling of the pulmonary microenvironment during Paragonimus proliferus infection in a rat model

Paragonimiasis, a food-borne zoonosis caused by Paragonimus spp., can cause severe pulmonary inflammation and fibrosis. However, relationships among the host, parasite and lung microbiome at defined infection stages remain poorly understood. We compared independent groups of rats...

Effectiveness of OBE-oriented and online-offline blended teaching versus traditional lecture-based teaching in nuclear medicine nursing education: a multicenter prospective comparative study

Nuclear medicine nursing demands high professional competence, particularly in radiation protection and radiopharmaceutical management. Traditional lecture-based teaching (LBT) fails to meet modern training needs. Outcome-Based Education (OBE) and online-offline blended teaching...

Density Gradient Centrifugation Versus Swim‐Up for Cumulative Live Birth in IVF/ICSI Cycles With High Sperm DNA Fragmentation: A Propensity Score Matching Analysis

BACKGROUND: A high sperm DNA fragmentation index (DFI) has been found to be associated with male infertility and suboptimal outcomes in assisted reproductive technology (ART). Although sperm preparation techniques can reduce DFI following processing, it is unclear whether density...

Helicobacter pylori genotypes, virulence and antibiotic resistance patterns among outpatients with gastric diseases in representative urban-rural areas of Yunnan, China

Abstract Background The epidemiological and molecular characteristics of Helicobacter pylori ( H. pylori ) across different gastric lesion stages remain unclear. This study aimed to analyse H. pylori positivity, phylogenetic lineages, virulence genotypes, and antibiotic resistanc...

Ellagic Acid Enhances RSL3 ‐Induced Ferroptosis by Inhibiting the Nrf2/ HO ‐1 Signaling Pathway in Pancreatic Ductal Adenocarcinoma

Pancreatic ductal adenocarcinoma (PDAC) exhibits profound therapeutic resistance due to redox adaptation, particularly through Nrf2/HO-1 pathway activation. Ferroptosis induction via GPX4 inhibitors (e.g., RSL3) is promising but limited by adaptive antioxidant responses. Ellagic...

Longitudinal associations between cardiovascular-kidney-metabolic stage and cognitive trajectories among middle-aged and older Chinese adults

Cardiovascular–kidney–metabolic (CKM) dysfunction and cognitive decline frequently coexist, but the temporal direction and potential biomarker pathways linking stage-based CKM burden to cognitive change remain uncertain. We analysed 5547 adults aged 45 years or older from the 201...

Cannabis sativa L. juice as a novel functional drink ameliorates hyperlipidemia by inhibiting lipid synthesis and oxidative stress

Hyperlipidemia is a major public health concern due to its increasing global prevalence. Hemp seed ( Cannabis sativa L. seed) is a medicinal plant that has gained attention for its potential applications under the “homology of medicine and food” framework. Previous studies sugges...

Emotional and behavioral problems in children of parents with severe mental illness in Wuhan, China: the roles of child maltreatment and exposure to parental victimization

Guided by a developmental-ecological and trauma-informed framework, children of parents with severe mental illness (SMI) may develop emotional and behavioral problems (EBPs) through inherited vulnerability, disrupted caregiving, socioeconomic adversity, stigma, and trauma-related...

Neoadjuvant Anbenitamab and HB1801 in ERBB2 -Positive Breast Cancer

IMPORTANCE The combination of neoadjuvant taxanes with trastuzumab and pertuzumab remains the cornerstone treatment strategy in ERBB2 -positive breast cancer. Anbenitamab (a ERBB2 -biparatopic antibody that induces profound receptor clustering) and HB1801 (a solvent-free albumin-...

Efficacy and Safety of Recibokibart, an Interleukin-36 Receptor Antibody, in Generalized Pustular Psoriasis: A Phase 2 Randomized Trial

BACKGROUND: Generalized pustular psoriasis (GPP) is a rare, severe inflammatory skin disease characterized by acute flares. Recibokibart is a humanized IgG1 monoclonal antibody targeting the interleukin-36 (IL-36) receptor. In a phase 1b open-label study, a single intravenous dos...

Genomic and transcriptomic landscape of overweight Chinese breast cancer reveals NOTCH2 mutation–mediated endocrine resistance and therapeutic strategies

Abstract Background Obesity (OB) and overweight (OW) are common in patients with breast cancer and are associated with inferior outcomes, yet their impact on tumor molecular architecture remains incompletely understood. This study aimed to define the genomic and transcriptomic fe...

Interpretable Machine Learning for Predicting Radiation-Induced Hypothyroidism in Head and Neck Cancer: A Dual-Center Retrospective Study

Background/Objectives: Radiation-induced hypothyroidism (RIHT) is a frequent complication in head and neck cancer (HNC) patients following radiotherapy, significantly impacting quality of life. This study aimed to develop and validate an interpretable machine learning model for p...

Nigral DJ-1 gene knockout in tree shrews can induce phosphorylated α-synuclein aggregation and all key phenotypes of Parkinson’s disease

Abstract DJ-1 is a genetic factor associated with Parkinson’s disease (PD), and altered DJ-1 function has been implicated in PD pathogenesis. However, DJ-1 knockout mice fail to reproduce robust PD-like phenotypes or neuropathology, leaving the contribution of DJ-1 deficiency to...

Complete genome-derived metabolic interactions reveal the impact of gut ecology on human health

Abstract Metabolic interactions govern gut microbiome assembly, yet functional rules remain obscured by genomic incompleteness and fragmentation. Here, we leverage 1,150 complete genomes to construct genome-scale metabolic models, demonstrating that draft genomes introduce system...

Salt-tolerant β-glucosidases from Karamay saline soil for soybean isoflavone bioconversion

β-Glucosidase (EC 3.2.1.21) hydrolyzes β-glycosidic bonds and is widely used in the food, fuel, and pharmaceutical industries. It holds significant potential particularly for converting glycosides, such as soybean isoflavone glycosides and ginsenosides, into their more bioactive...