Duke University Hospital

Non‐Weightbearing in Hinged‐Knee Braces Locked in High Degrees of Flexion Shows High Hamstring Muscle Activation in Healthy Volunteers

Purpose To determine the effect of various postoperative ambulation and bracing protocols on hamstring muscle group activation in healthy volunteers. Methods Hamstring muscle group activation was assessed via Myontec Mbody 3 Shorts. Volunteers ambulated 10 yards in conditions inc...

Monoclonal antibodies inhibiting MMP-9 activity alleviate peripheral neuropathy in diabetic mice

Diabetic peripheral neuropathy (DPN) is a debilitating medical condition that affects millions of individuals, causing neuropathic pain and chronic wounds. Despite its substantial impact, there are currently no effective disease-modifying treatments available to prevent or revers...

Randomised phase III trials of cancer prevention drugs: a systematic review and prediction of trial success

BACKGROUND: Pharmacological cancer risk reduction has received far less investment than therapeutic oncology and only a few agents have entered routine practice. Understanding the predictors of positive outcomes of previous trials and issues faced is critical to future developmen...

An Updated Evidence Assessment of the Genetic Causes of Dilated Cardiomyopathy

BACKGROUND: Evidence of the diverse genetic architecture of dilated cardiomyopathy (DCM) continues to emerge and requires reassessment of the clinical relevance of implicated disease genes. Building on the 2019-2020 Clinical Genome Resource evaluation, the DCM gene curation exper...

Estrogen promotes tumor phenotypes in ER+ and ER– breast cancer through UGDH-GPR30

Estrogen can promote aggressive tumor phenotypes in estrogen receptor–positive (ER + ) breast cancer; however, ER – cell lines are not widely considered estrogen responsive. Noncanonical estrogen-stimulated pathways such as the membrane-bound G protein–coupled estrogen receptor (...

Machine Learning Guided Discovery of Microbiome Metabolites That Inhibit HDAC

Abstract Histone deacetylase (HDAC) is a family of key epigenetic regulator implicated in inflammation, metabolism, and cancer. Microbiome metabolites can modulate host histone acetylation, yet systematic identification of metabolites that target HDAC remains limited. Here we pre...

Development and Assessment of Poly(ethylene glycol) Diacrylate-Embedded Metalloporphyrin for Phosphorescence Lifetime-Based In Vivo Monitoring of Oxygen Tension

Abstract The timely detection of ischemia is crucial for enhancing outcomes in reconstructive surgery. This study presents the development and in vivo evaluation of a metalloporphyrin-based phosphorescence lifetime oxygen sensor embedded in poly(ethylene glycol) diacrylate (PEGDA...

Care Models for the Genetic Evaluation of Dilated Cardiomyopathy at Sites of the DCM Consortium

Abstract Background Clinical genetic evaluation for patients with dilated cardiomyopathy (DCM) is minimally implemented and models of care are not defined. To understand current genetics care for DCM, a systematic needs assessment was conducted. Methods Principal Investigators (P...

MeTime: An R package for reproducible longitudinal metabolomics data analysis

Abstract MeTime is an opensource R package for reproducible analysis of longitudinal metabolomics data. It builds upon a central S4 container, metime_analyser, that stores multiple datasets, associated metadata and analysis outputs, enabling unified handling of complex longitudin...

Association of peri-reperfusion oxygen exposure and early allograft dysfunction after deceased donor orthotopic liver transplantation: a retrospective cohort study

Hepatic insufficiency within a week of orthotopic liver transplantation (OLT), termed early allograft dysfunction (EAD), occurs in 20–25% of deceased donor OLT recipients and carries substantial morbidity and mortality. Ischemia reperfusion injury, in which reperfusion after isch...

Characterization of mitochondria in trophoblast cells derived from control and early-onset preeclamptic pregnancies via induced pluripotent stem cells

Abstract Early embryonic development occurs in a low oxygen environment, and mitochondrial morphology and function are distinct in embryonic cells and pluripotent stem cells (PSC), which rely less on oxidative phosphorylation than differentiated cells. Oxidative phosphorylation i...

Deletion of P311 enhances regeneration in aged skeletal muscle

While aging results in marked decreases in the capacity of skeletal muscle to repair following injury, as well as increases in fibrosis, the pathways and factors that regulate these processes are poorly defined. P311/Nrep (P311) is a highly conserved protein known to promote fibr...

Do Patients Demonstrate Improved Patient-Reported Outcome Measures Following Revision Shoulder Arthroplasty Relative to Their Pre-Primary Condition? A Retrospective Case Series

Background: No study has evaluated patient-reported outcome measures (PROMs) from before primary total shoulder arthroplasty (TSA) to after revision TSA to determine if patients felt that they were better off than prior to undergoing primary TSA. Understanding this longitudinal t...