Wuhan Polytechnic University

Berberine Protects Brain Endothelial Barrier via β-Catenin: A Network Pharmacology in Pigs and In Vitro Mechanistic Analysis

Glaesserella parasuis (G. parasuis) is an important swine pathogen that causes Glässer’s disease, of which meningitis is among the most severe clinical symptoms. However, the molecular mechanisms underlying G. parasuis-induced meningitis remain poorly understood. In this study, w...

GPU-accelerated parallel strategies for multiscale isogeometric topology optimization

In multiscale isogeometric topology optimization (ITO), the highly coupled cross-scale sensitivity analysis and the wide support of NURBS basis functions constitute the dominant computational bottleneck. To address these computational bottlenecks, this paper proposes a Decoupled...

Cleaner Processes, Safer Products: Environmental and Public Health Pathways for Chinese Herbal Medicine

Chinese Herbal Medicines (CHMs) are at the crossroads of therapeutic practice, agricultural biodiversity, industrial production, and health. Traditional methods of CHM production, such as decoction, solvent extraction, evaporation, and purification, can be efficient but can use l...

Longitudinal associations among exercise atmosphere, physical self-esteem, and aging attitudes in older adults

Physical self-esteem and aging attitudes are two key psychological factors associated with well-being in older adults. While previous research has emphasized the role of exercise in promoting healthy aging, how these factors dynamically interact within varying exercise environmen...

Biocatalytic Synthesis of dNTPs from Bacterial Biomass

Abstract Deoxyribonucleoside triphosphates (dNTPs) are crucial reagents widely utilized in nucleic acid amplification. However, current synthesis methods are often plagued by environmental pollution for chemical synthesis and the high costs of phosphate donors for enzymatic synth...

Experimental study on the effect of vibration on local reverse polarity and current density distribution in proton exchange membrane fuel cells

Vibrations impair the structural stability and water management of proton exchange membrane fuel cells (PEMFCs). This induces uneven current density distribution (CDD) and triggers local reverse polarity (LRP) events. These phenomena further exacerbate performance deterioration a...

A phase II trial of chemotherapy-free neoadjuvant camrelizumab plus apatinib for resectable HNSCC: pathological response and vascular-immune crosstalk

Abstract Purpose: To evaluate the efficacy, safety, and tumour microenvironment remodelling of neoadjuvant camrelizumab plus apatinib in resectable head and neck squamous cell carcinoma (HNSCC; stage II–IVA). Materials and Methods: In this single-arm, exploratory phase II trial (...

Frequency Control Strategy for High-Penetration Renewable Power Systems with Voltage-Sensitive Flexible Load Clusters

The high penetration of renewable energy has posed significant challenges to frequency regulation, primarily due to the scarcity of traditional regulation resources in these regions. To address this, a novel secondary frequency regulation control method utilizing voltage-sensitiv...

Application of CoQ10-loaded gSP-CS double-layer emulsion in milk: Impacts on quality and storage stability

The application of coenzyme Q 10 (CoQ 10 ) in liquid milk remains challenging due to its poor water solubility and chemical instability. Although CoQ 10 -loaded emulsions have been extensively studied, a critical knowledge gap persists: how exogenous emulsion droplets coexist wit...

Analytical Study for Transient Infiltration of Water in Vegetated Landfill Cover under a Time-Varying Rainfall Scenario

Abstract Vegetated landfill covers (VLC) are extensively applied to block rainwater infiltration and reduce the leachate generation. However, the associated analytical works remain limited. This study, for the first time to our best knowledge, develops analytical solutions for tr...

The complete chloroplast genome sequence and phylogenetic position of Mallotus apelta

Mallotus apelta is a perennial species of Euphorbiaceae widely distributed in subtropical East and Southeast Asia. In this study, we sequenced and analyzed its complete chloroplast genome. The genome was 164,055 bp in length and exhibited a typical quadripartite structure, compri...

Spatiotemporal Evolution, Spatial Heterogeneity and Driving Mechanisms of Agricultural Carbon Emissions in the Main Grain-Producing Areas of the Middle Reaches of the Yangtze River, China

Achieving low-carbon agriculture in major grain-producing areas hinges on balancing emission reduction imperatives with the need to maintain food security. However, previous studies have largely examined agricultural carbon emissions (ACE) at administrative scales, potentially ov...

Endangered Salvia daiguii Has Evolved Drought Tolerance Through Photosynthetic, Antioxidant, and Respiratory Adaptations: Implications for Ex Situ Propagule Reintroduction

Salvia daiguii is an endangered plant species with medicinal potential, whose ex situ conservation and reintroduction are essential for population restoration. However, drought tolerance, critical to the survival and establishment of reintroduced populations, remains poorly under...

The complete chloroplast genome of Cynanchum hemsleyanum and its phylogenetic analysis

C. hemsleyanum chloroplast genome is 157,356 bp with a quadripartite structure, 37.99% GC, and 132 genes (87 protein-coding, 37 tRNA, 8 rRNA). Phylogenomic analysis places it as sister to C. thesioides with 100% bootstrap support. This resource aids molecular identification, gene...

Integrated Characterization of ceRNA (circRNA/lncRNA/miRNA/mRNA) Regulatory Networks in a Glaesserella parasuis–Induced Mouse Meningitis Model

Glaesserella parasuis (G. parasuis, GPS) is a known causative agent of meningitis. A growing body of research indicates that non-coding RNAs (ncRNAs) are deeply involved in modulating inflammatory responses. In our study, we profiled the expression changes in circRNAs, lncRNAs, m...

CIAFNet: An RGB-D Cross-Modal Interaction and Adaptive Fusion Network for Camellia oleifera Fruit Detection

To better address the accuracy bottleneck of RGB-only Camellia oleifera C.Abel fruit detection in complex orchard environments, this paper proposes CIAFNet—a dual-stream RGB-D fusion detection network—and evaluates its potential as a visual front end for relative 3D localization...

A One-Pot Reverse Transcriptase-Mediated, Pre-amplification-Free CRISPR/Cas12a Assay for Ultrasensitive Nucleic Acid Detection

Abstract CRISPR/Cas12a holds great promise for biosensing and diagnostics, but conventional methods suffer from low catalytic efficiency, high background, and reliance on pre-amplification. Direct detection of structured RNAs also remains challenging. Herein, we report the develo...

Bitterness and Salivary Lubrication Properties ofSoyasaponin Acid Hydrolysis Products

Abstract Soyasaponins (Ssa) are strongly bitter, limiting food applications. In this study, acid hydrolysis generated secondary Ssa and aglycones, characterized by HPLC–TOF–MS. Bitterness decreases upon hydrolysis, with the sensory score increasing from 71.3 for native Ssa to 85....

Effects of Electronic Layout and Beam Design on High-Speed Dynamic Characteristics of FFF 3D Printers

High-speed Fused Filament Fabrication (FFF) printers are prone to nozzle vibration caused by moving-part inertia, frame flexibility, and modal coupling during high-acceleration motion, which can reduce deposition-trajectory stability. This study evaluates the dynamic adaptability...

Chemo-Enzymatic Synthesisof Purine 3′-O-Amino-DeoxyribonucleosideTriphosphates

Abstract 3′-O-amino-deoxyribonucleoside triphosphates (3′-ONH2-dNTPs) are crucial reversible terminators for template-independent DNA synthesis. Owing to the structural complexity of purine bases, here we propose a chemo-enzymatic process for the synthesis of purine-type 3′-ONH2-...