Wuhan Polytechnic University

Bitterness and Salivary Lubrication Properties ofSoyasaponin Acid Hydrolysis Products

Abstract Soyasaponins (Ssa) are strongly bitter, limiting food applications. In this study, acid hydrolysis generated secondary Ssa and aglycones, characterized by HPLC–TOF–MS. Bitterness decreases upon hydrolysis, with the sensory score increasing from 71.3 for native Ssa to 85....

A One-Pot Reverse Transcriptase-Mediated, Pre-amplification-Free CRISPR/Cas12a Assay for Ultrasensitive Nucleic Acid Detection

Abstract CRISPR/Cas12a holds great promise for biosensing and diagnostics, but conventional methods suffer from low catalytic efficiency, high background, and reliance on pre-amplification. Direct detection of structured RNAs also remains challenging. Herein, we report the develo...

Effects of Electronic Layout and Beam Design on High-Speed Dynamic Characteristics of FFF 3D Printers

High-speed Fused Filament Fabrication (FFF) printers are prone to nozzle vibration caused by moving-part inertia, frame flexibility, and modal coupling during high-acceleration motion, which can reduce deposition-trajectory stability. This study evaluates the dynamic adaptability...

Chemo-Enzymatic Synthesisof Purine 3′-O-Amino-DeoxyribonucleosideTriphosphates

Abstract 3′-O-amino-deoxyribonucleoside triphosphates (3′-ONH2-dNTPs) are crucial reversible terminators for template-independent DNA synthesis. Owing to the structural complexity of purine bases, here we propose a chemo-enzymatic process for the synthesis of purine-type 3′-ONH2-...

Adipose Tissue Palmitoylation Cycling Mediates Insulin Resistance and Preservation of β-Cell Function in Mice

Excess adiposity is associated with insulin resistance and pancreatic β-cell dysfunction. We show that palmitoylation cycling in white adipose tissue (WAT) balances insulin sensitivity and insulin secretion. WAT and brown adipose tissue (BAT) ablation of acyl-protein thioesterase...

A tributyrin-monolaurin complex protects piglets against PEDV infection via viral inhibition, lipid metabolism restoration, and microbiota modulation

Porcine epidemic diarrhea virus (PEDV) is a major pathogen causing severe diarrhea and intestinal damage in piglets. Monolaurin (GML) and tributyrin (TB) are well-documented functional lipids with intestinal health benefits, but their combined efficacy against PEDV infection rema...

Efficient preparation of fatty acid methyl esters using liquid lipase from thermotolerant fungus Rasamsonia emersonii via synergistic strategies

Lipase-catalyzed biodiesel preparation in non-aqueous media has the advantages of reduced energy consumption, simplified recovery of fatty acid methyl esters (FAMEs), and minimized environment pollution, etc. To discover a novel thermophilic lipase, achieve its high-level express...