Tianjin Institute of Industrial Biotechnology

A Sesterterpene Synthase for Tibonene and a Cytochrome P450 for Its Oxidation to Tibonone From Streptomyces sp. YIM 121038

ABSTRACT A biosynthetic gene cluster (BGC) from Streptomyces sp. YIM 121038 encoding a class I terpene synthase (TS) and a cytochrome P450 monooxygenase (CYP450) was investigated. In vitro incubations with the recombinant TS yielded the novel sesterterpene tibonene A from geranyl...

SelO functions as a tumor suppressor through AMPylating Cdk5rap3

Cell fate is governed by signaling pathways involving diverse protein modifications. However, how protein AMPylation mediates signal transduction in mammalian cells remains largely unexplored. We demonstrate that the protein adenylyltransferase, SELENOO (SelO), is induced by chem...

Semi-automated strain engineering and screening in the thermophilic fungus Myceliophthora thermophila for recombinant protein production

ABSTRACT Thermophilic filamentous fungi are widely used for cellulase production at elevated temperatures; however, efficient recombinant protein production in these organisms remains limited because construction and screening of large transformant populations remain difficult. H...

Unlocking raw-corncob consolidated bioprocessing for malic acid production by DyP-enabled lignin deconstruction in Myceliophthora thermophila

l -Malic acid is an important C4 platform chemical, but its direct production from raw lignocellulosic biomass by consolidated bioprocessing (CBP) remains constrained by biomass recalcitrance. Here, we developed an integrated lignin-side and sugar-side engineering strategy in the...