Shanghai Ninth People's Hospital

Expert Consensus on Robot-Assisted Vascularized Bone Grafting for the Treatment of Early-Stage Osteonecrosis of the Femoral Head

Osteonecrosis of the femoral head (ONFH) remains a progressive clinical challenge that predominantly affects young and active adults between 20 and 50 years of age. Without timely and effective surgical intervention, natural disease progression leads to structural subchondral fra...

3D Microfluidic Networks Formed by Mechanically Guided Compressive Buckling for Large‐Scale Perfused Tissue Culture

ABSTRACT Engineered tissues and organoids offer transformative potential for drug screening and regenerative medicine, yet their application is constrained by limited size and slow maturation. Despite the development of various channel networks for large‐scale engineered tissue p...

“Precision planning for tissue expansion: A three-dimensional scanning-based predictive model to guide surgical timing”

Tissue expansion remains an essential reconstructive technique, yet the timing of expander removal is still largely determined by subjective clinical judgment. We developed a three-dimensional (3D) scanning-based quantitative workflow to objectively monitor expansion progress and...

Upadacitinib in East Asia: Translating Trial Results Into Maintenance Care

Data sharing is not applicable to this article as no new data were created or analyzed in this study.

Efficacy, Efficiency, and Safety of a Poly-L-lactic Acid Cuff Tube Assisting for Microvascular Anastomosis

BACKGROUND: Vascular anastomosis is a cornerstone of microsurgery. Anastomosis time and vessel patency significantly influence surgical success. The disadvantages of the traditional hand-sewn method requires a high technical threshold, prolonged surgical time, and an increased ri...

CD24 blockade reprograms macrophages and synergizes with CD47 blockade to overcome tumor immune evasion

Although immunotherapy has revolutionized cancer treatment, only a minority of head and neck squamous cell carcinoma (HNSCC) patients derive clinical benefit, underscoring the urgent need to uncover novel immune evasion mechanisms. CD24 serves as a pivotal innate immune checkpoin...

Copper-based targeted nanozyme platform with sequential phototherapy-enzymatic therapy for orchestrating macrophage homeostasis in rheumatoid arthritis

Rheumatoid arthritis (RA) remains a formidable clinical challenge due to the vicious cycle of hyperactivated M1 macrophages, excessive reactive oxygen species (ROS), and sustained pro-inflammatory cytokine storm. Herein, we rationally engineered a targeted copper-based nanozyme p...

Following patient questions across clinical and evidence services with large language model assistance

Preprint. Not peer reviewed. For people with multimorbidity, completing an evidence task does not ensure that the question raised in care has been answered. We propose requirements for following an individual patient question across clinical and evidence services. A continuing re...

Following patient questions across clinical and evidence services with large language model assistance

Preprint. Not peer reviewed. For people with multimorbidity, completing an evidence task does not ensure that the question raised in care has been answered. We propose requirements for following an individual patient question across clinical and evidence services. A continuing re...

Why Peri‐Implant Tissues Are Susceptible to Inflammatory Breakdown—Single‐Cell Transcriptomics Reveals Immune Priming in Clinically Healthy Peri‐Implant Mucosa and CXCR2 + Neutrophil Activation in Peri‐Implantitis

ABSTRACT Aim Peri‐implant tissues are more susceptible than gingiva to rapid inflammatory breakdown, but the cellular basis of this vulnerability remains unclear. This study investigated (1) whether clinically healthy peri‐implant mucosa already displays an immune landscape disti...

Different concentrations of atropine in children with high myopia: a 2-year multicenter double-blind, randomized controlled trial

High myopia in children risks irreversible visual impairment, yet optimal interventions remain debated. This multicenter double-blind, randomized-controlled trial aims to evaluate the effect and safety of different atropine eyedrops for myopia control in children with high myopia...

Consensus recommendations for neurofibromatosis type 1 management in Asia Pacific

Neurofibromatosis type 1 (NF1) is a complex multisystem disorder requiring coordinated multidisciplinary care. This study aimed to develop consensus recommendations for NF1 management in the Asia-Pacific region. A modified Delphi process involving two survey rounds and a virtual...

DentalGEN: a large-scale controllable generative AI framework for automated dental crown restoration

Dental crown restoration addresses a global public health burden, yet is constrained by a slow, variable, and labor-intensive manual design process that limits access to high-quality treatment. Here we introduce DentalGEN, a large-scale controllable generative artificial intellig...

EaMIC3 inhibits cell apoptosis and facilitates Eimeria acervulina infection through interacting with EpCAM

Coccidiosis, caused by Eimeria acervulina, is a pathogenic protozoan disease, leading to huge economic losses to the global poultry industry. At the early stage of E. acervulina infection, microneme protein 3 (EaMIC3) is involved in the inhibition of infected host cell apoptosis,...

The clinicopathological significance and mechanisms of interstitial foam cells formation in patients with primary membranous nephropathy

Accumulation of foam cells (FCs) in interstitium is frequently observed in membranous nephropathy (MN). However, the pathophysiological significance of those cells remains poorly understood. It is generally accepted that interstitial FCs are derived from macrophages with lipid de...

A positive feedback loop between HNRNPF and GRHL2 drives NEK8-mediated prostate cancer progression

Prostate cancer (PCa) remains a major cause of cancer-related mortality in men, especially in advanced stages. The role of Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) in PCa progression is poorly defined. The function and mechanism of HNRNPF were investigated using in vitr...

Protocol to prepare PARPi-combined LIN28B siRNA nanoparticles and evaluate therapeutic efficacy of malignant ascites

Malignant serous effusion (MSE) is a life-threatening complication of advanced cancers with limited effective treatments. Here, we present a protocol for the preparation of LIN28B siRNA-loaded nanoparticles and their combination with PARP inhibition for therapeutic evaluation in...

Multiomics analysis reveals super enhancer associated GTSF1 and Schwann cell heterogeneity in neuroblastoma

Super-enhancers (SEs) contribute to oncogenic transcriptional programs in neuroblastoma (NB), but their relationship with nerve-associated microenvironmental remodeling remains unclear. This study integrated epigenomic, transcriptomic, dependency, single-cell, and clinical valida...

Preparing large biological specimens for high-throughput volume electron microscopy

Abstract To enable high-throughput volume electron microscopy (vEM) imaging, osmium-based staining methods are routinely employed to enhance membrane contrast and electrical conductivity in biological specimens. Nevertheless, reliable preparation of densely and uniformly stained...

Integrated postmortem brain transcriptomics identifies a five-gene Alzheimer’s disease-associated signature with a reproducible whole-blood CEBPB correlate

Transcriptomic profiling provides a systems-level view of Alzheimer’s disease (AD), capturing coordinated neuronal, glial, vascular, and immune-related changes. Yet the same breadth that makes transcriptomics informative also complicates interpretation because technical heterogen...