Qilu Hospital of Shandong University

Laparoscopic−assisted versus purely transanal Soave procedures for Hirschsprung disease in children aged ≤ 4 years: an age−stratified retrospective study

Laparoscopic−assisted and purely transanal Soave procedures are commonly used for Hirschsprung disease, but whether their comparative outcomes differ according to age remains uncertain. This single−center retrospective cohort study included 188 children aged ≤ 48 months who under...

Triple-Encoding Platform for Multiplexed In Situ Profiling of Extracellular Vesicle miRNAs Enables Precision Diagnosis of Ovarian Cancer

Abstract Ovarian cancer, the most lethal gynecologic malignancy, urgently demands early and accurate diagnostic methods. Tumor-derived extracellular vesicles (tEVs) hold great promise as noninvasive biomarkers, yet their clinical translation is severely hampered by inherent heter...

The cardiovascular–liver–metabolic syndemic and mortality and life expectancy: two prospective cohort studies

Cardiovascular disease (CVD), metabolic dysfunction–associated steatotic liver disease (MASLD), type 2 diabetes mellitus (T2DM), and obesity frequently coexist and share overlapping pathophysiologic mechanisms, collectively constituting a cardiovascular–liver–metabolic (CLM) synd...

Drug Repurposing for Treating NCOR‐Related Intellectual Disability

ABSTRACT Nuclear receptor corepressors (NCOR1/2) mediate the transcriptional activity of methyl‐CpG binding protein 2 (MECP2), and genetic variants in NCOR1/2 or MECP2 associate with autism or intellectual disability. However, it is unknown whether heterozygous NCOR mutations imp...

Development and validation of machine learning models for preoperative prediction of perioperative outcomes in patients with thymic epithelial tumors

Thymic epithelial tumors (TETs) are rare anterior mediastinal tumors with heterogeneous perioperative recovery. We developed and externally validated machine-learning models using preoperative and preoperatively available variables to predict postoperative complications, prolonge...

Biomechanical comparison of two-, three-, and four-screw zero-profile ACDF constructs: effects of screw number and orientation

Zero-profile anterior cervical discectomy and fusion (ACDF) constructs vary in screw number and trajectory, but their effects on immediate stability and load transfer remain unclear. This study compared two-, three-, and four-screw constructs and examined middle-screw orientation...

Gut microbiota‐derived tryptophan metabolite attenuates calcium oxalate nephropathy by suppressing renal tubular cell apoptosis through the AhR – SOCS3 – STAT1 axis

Calcium oxalate (CaOx) nephropathy is a highly prevalent urological disease worldwide. Gut microbiota dysbiosis and host metabolic dysregulation are recognized as pivotal drivers in disease pathogenesis, yet the underlying mechanisms remain incompletely understood. In this study,...

Sex‐Specific Digital Phenotyping of Lower Urinary Tract Symptoms: A Cross‐Sectional Study of 1586 Adults From 35 Centers in China

ABSTRACT Background and Purpose Lower urinary tract symptoms (LUTS) represent a complex clinical syndrome with significant sexual dimorphism. Traditional “one‐size‐fits‐all” management often overlooks intra‐sex phenotypic heterogeneity. This study utilized unsupervised machine le...

Osteoporosis knowledge and its associations with health beliefs, self-efficacy and activities of daily living in patients with knee osteoarthritis: a cross-sectional study

Osteoporosis knowledge is relevant to bone-health behaviour and fracture prevention in older adults with knee osteoarthritis (KOA). This study examined associations of osteoporosis-related health beliefs, domain-specific self-efficacy and activities of daily living (ADL) with ost...

Organic–Inorganic Dual‐Nanofiber Interpenetrating Network Scaffolds for Complementary Enhancement of Mechanical Stability and Osteogenic Bioactivity

ABSTRACT Incorporating nanofibers into 3D‐printed scaffolds is an effective strategy to enhance the physicochemical properties and osteogenic bioactivity of bone tissue engineering constructs. In this study, we developed a composite scaffold featuring a dual‐nanofiber interpenetr...

Symptom‐System Complexity and Network‐Informed Assessment Priorities in Patients With Colorectal Cancer and a Stoma: A Multicentre Cross‐Sectional Study

ABSTRACT Aims and Objectives To characterise symptom‐system heterogeneity and entropy‐informed complexity and identify network‐informed assessment priorities for clinical nursing in patients with colorectal cancer and a stoma. Background Patients with colorectal cancer and a stom...

Phloroglucinol inhibits human ureteral contractions via activation of cAMP‐PKA pathway

Abstract Background and Purpose Phloroglucinol is used to treat renal colic, especially when non‐steroidal anti‐inflammatory drugs are contraindicated. This study aimed to clarify its mechanism by examining effects on human ureteral contractility and underlying signalling pathway...

Integrated Multi‐Omics Reveals Cellular States and Microenvironmental Remodeling in Coexisting DCIS and IDC

ABSTRACT Ductal carcinoma in situ (DCIS) is a non‐invasive precursor of invasive ductal carcinoma (IDC), yet the biological mechanisms underlying the transition from DCIS to IDC remain incompletely understood. Here, we integrate spatial transcriptomics, single‐cell RNA sequencing...

Ammonia Accumulation Drives Intervertebral Disc Degeneration by Triggering Ammonia‐Induced Cell Death Through Lysosome–Mitochondria Crosstalk

ABSTRACT Intervertebral disc degeneration (IVDD) is a major cause of low back pain and is closely associated with metabolic imbalance within the disc microenvironment, yet the underlying mechanisms remain poorly understood. Here, we identify ammonia accumulation as a metabolicall...

Design of Dual-Path Antibacterial Aluminum Alloy Based on ZnO Sputtering and Surface Roughness Control

Abstract Antibacterial alloys based on aluminum alloys have become a hot topic. However, most existing research is limited to a single antibacterial mechanism and may suffer from insufficient long-term efficacy, making it difficult to meet the requirements of large-scale producti...

PediatricLiver score: a novel risk score for screening elevated liver stiffness in the pediatric population (CHESS2401)

Given the looming crisis of rapid increase in childhood obesity globally, chronic metabolic diseases have consequently emerged but without sufficient awareness among children. Reliable and accessible screening tools thus are required to screen for significant liver fibrosis in th...

IL-2-mediated Treg cells recruitment regulates neuroimmune crosstalk to promote hippocampal neurogenesis in depressive mice

Major depressive disorder (MDD) is a prevalent and debilitating neuropsychiatric disorder characterized by severe neuroinflammation and impaired hippocampal neurogenesis, yet effective clinical treatments remain limited. Accumulating evidence indicates that adaptive immune dysfun...

Beyond statistical thresholds: Mapping sequential ATN cascade transitions via brain structural topography in Alzheimer’s disease

Alzheimer’s disease is widely conceptualized as a biological continuum initiated by amyloid-β (Aβ) accumulation, followed by tau aggregation and neurodegeneration. Although the ATN framework is commonly used to define disease stages, its static biomarker categories may not fully...

Integrating neuroimaging, transcriptomics and single-cell sequencing identifies candidate molecular features of cortical vulnerability in amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a rapidly progressive neurodegenerative disease that leads to reductions in cortical surface area (SA) and thickness (TH). However, the genetic and molecular mechanisms underlying these cortical structural alterations remain unclear. This st...

Enhanced mortality prediction using frailty models in cardiac surgery patients: evidence from the MIMIC-IV database

The Frailty Index based on Laboratory Tests (FI-Lab) and the Hospital Frailty Risk Score (HFRS) represent two frailty measures, yet their comparative and combined prognostic utility in cardiac surgical populations remains unclear. We conducted a retrospective cohort study using t...