Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

Development of a polyclonal antibody-based sandwich ELISpot assay for interleukin-4/13B1 in rainbow trout Oncorhynchus mykiss

Abstract In mammals, interleukin-4 (IL-4) and IL-13 are important Th2 cytokines that enhance antibody production by B cells and proliferation of Th2 cells. Salmonid fish have three homologs to both of these cytokines as a result of four rounds of whole genome duplication events,...

Deciphering the genomic signatures of climate vulnerability across 77 sheep breeds

Abstract Among domesticated species, sheep ( Ovis aries ) have achieved a wide geographic distribution due to their mobility, rusticity and ability to adapt to diverse environments and climatic conditions. Here, we characterise the genome-wide diversity of >1,000 sheep representi...

‘PePApipe’: A complete bioinformatics analysis pipeline for African Swine Fever Virus genome

African Swine Fever Virus (ASFV) is of high concern in porcine livestock across the world due to both the high mortality rates and the trade restrictions imposed on affected regions. The viral genome is large and complex, and genomic analysis is essential for tracing its origin a...

Unveiling the interactions between design optimization, modeling strategies, and spatial analysis in genomic selection

Abstract Key message Partially-replicated designs are more efficient than fully-replicated ones. They can be further enhanced through optimization and present positive synergy with fully-efficient models and 2D P-splines. Abstract In plant breeding, traditional field trials rely...

Soil health assessment using buried cotton underpants with the help of 1000 citizen scientists

Societal Impact Statement Soil is a vital resource for life on Earth. Although many soils are degraded, societal awareness of soil‐related issues is limited. To highlight soil as a key resource and fascinating study system, we initiated a citizen science project together with 100...

Simulated climate change disrupts soil food webs and agroecosystem services in two contrasting Mediterranean drylands

Climate change and intensive agriculture are major threats to soil biodiversity and key ecosystem functions, and Mediterranean systems are particularly vulnerable to these global change drivers. In such threatened environments, gaining insight into the interactions between aboveg...

Effect of planting density on the agronomic performance and forage potential of Vicia sativa L. in the high Andes of Peru

In the high Andean regions of Ancash, Peru, limited high-protein forage during dry seasons challenges livestock feeding. A locally adapted ecotype of common vetch ( Vicia sativa L.) grows spontaneously and shows resilience to harsh conditions. This study evaluates its agro-morpho...

Inoculation with Trichoderma in Coffea arabica Seedlings: Effects on Morphological Indices and Seedling Quality Under Nursery Conditions

Microbial inoculants such as Trichoderma have been proposed as sustainable tools to improve seedling quality in coffee nurseries; however, their effectiveness may vary according to formulation and genotype. This study evaluated three treatments: a solid Trichoderma formulation (1...