Institute of Molecular Biotechnology

Coxiella burnetii establishes a small cell variant (SCV)-like persistent form to survive adverse intracellular conditions

Abstract Coxiella burnetii is an obligate intracellular zoonotic bacterium that causes Q fever. Infections can be either acute or chronic. Of note, chronic Q fever develops months or years after primary infection without clinical symptoms, suggesting bacterial persistence. Yet, i...

Unified down-stream analysis of crosslinking mass spectrometry results with pyXLMS

Abstract Crosslinking mass spectrometry has become the method of choice for the identification of protein-protein interactions and for gaining insight into the structures of proteins in vivo. However, connecting crosslink search engine results with down-stream analysis tools, and...

Conformational asymmetry of replicated human chromosomes

Abstract DNA replication creates two sister chromatids that must acquire specific three-dimensional conformations to support genome function and stability. This organization is largely mediated by cohesin complexes, which extrude intra-chromosomal loops and link two chromatids, t...

Bioprocessing method is a critical factor for IgM oligomerization

Abstract Monoclonal immunoglobulins of subclass M pose great potential to fight diverse medical complications with high efficiency. We present a comparison of stable recombinant Chinese hamster ovary DG44 and K1 cell lines expressing IgM comprising κ-light chains and µ-heavy chai...

Deciphering the evolutionary origin of the enantioselectivity of short-chain dehydrogenases from plants toward 1-borneol

Abstract Enzyme engineering has produced numerous methods to optimize enzymes for biotechnological processes; however, less is known about how natural evolution creates new functionalities. We investigate the evolutionary emergence of enantioselectivity in plant borneol dehydroge...