Institute of Molecular Biology and Biophysics

In vivo CRISPR screening links NFKB1 to endocrine resistance in ER+ breast cancer

Resistance to endocrine therapy (ET) remains a major clinical challenge in the treatment of estrogen receptor-positive (ER+) breast cancer, underscoring the need for novel therapeutic targets. To identify genetic drivers of ET resistance, we conducted an in vivo genome-wide CRISP...

Resolving cell lineages and gene functions in the developing mouse gastrointestinal tract using in utero transduction

How diverse cell lineages emerge and are genetically regulated during organogenesis are central questions in understanding the developmental origins of disease. However, the mouse gut, including its intrinsic enteric nervous system (ENS) derived from migratory neural crest, has r...

Enantiomer-dependent biodistribution and exposure determine the in vivo behavior of a pleurocidin-derived antimicrobial peptide

ABSTRACT Antimicrobial peptides (AMPs) display rapid bactericidal activity in vitro , but have largely failed as systemically administered therapeutics, indicating that intrinsic potency alone does not predict in vivo efficacy. Here, we examine how pharmacokinetics, stereochemist...

Xylose phosphatase activity of dystroglycan self-regulates its receptor function

Dystroglycan (DG), a transmembrane receptor crucial for tissue development and pathogen entry, harbors a glycan composed of xylose and glucuronic acid called matriglycan. Loss of matriglycan or reduction in its length affects DG function, causing dystroglycanopathies. However, th...

LINGO1-targeted antibody-drug conjugates improve efficacy and tolerability of antineoplastic therapies in Ewing sarcoma models

We reported LINGO1 as a potential marker on Ewing sarcoma cells that could enable targeted drug delivery, improving treatment effectiveness while reducing side effects.