Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Synthesis of colloidal silver-polyphenols nanocomplexes - physicochemical properties, DFT calculation, modeling of adsorption and antibacterial activity

Abstract The aim of the studies was the investigation of nanocomplexes based on a silver core and attached three polyphenolic compounds belonging to tannins i.e. tannic acid (AgNPs-TA), 3,6-bis- O -di- O -galloyl-1,2,4-tri- O -galloyl- β -D-glucose (AgNPs-RT) and 1,2,3,4,6-penta-...

Fenofibrate, but not fenofibric acid, reduces fenestrations and impairs endocytic function in liver sinusoidal endothelial cells

Abstract Fenofibrate is widely prescribed for hyperlipidaemia and has been associated with rare but severe cases of drug-induced liver injury (DILI), yet its effects on liver sinusoidal endothelial cells (LSECs) remain to be investigated. LSECs maintain a highly permeable special...

Fate of post-blast residue in soils from an armed conflict zone determined by magnetic, microbiological, and ecotoxicological assessments

Weapon detonations generate substantial quantities of post-blast residue (PBR), which contributes iron-rich particles to soils. After deposition, these residues undergo redox transformations that produce bioavailable iron compounds, potentially increasing phytotoxic risks to agri...

Mammalian DNA maintenance methylation depends on histone but not PAF15 ubiquitination

In mammals, DNA methylation is an essential inheritable epigenetic modification maintained by the DNMT1-UHRF1 machinery in a UHRF1 E3 ubiquitin ligase-activity-dependent manner. UHRF1-catalyzed histone ubiquitination and PAF15 ubiquitination have been proposed to recruit DNMT1 to...

Snow microinvertebrates from lowlands, high mountains and the Arctic—implications for conservation efforts

Abstract The protection of cryospheric ecosystems and their biota cannot be properly defined without a detailed species inventory. Snow microinvertebrates are crucial for estimating cryophilic biodiversity, yet they are poorly described at broader spatial scales. To fill this kno...

Integrated Multi-Omics and Interactome Analysis of CDK8 Inhibition Reveals Erythroid Differentiation Programs and Therapeutic Synergy with BET Blockade in AML

Acute myeloid leukaemia (AML) is a therapeutically challenging malignancy driven by the self-renewal, quiescence, and therapy resistance of leukaemic stem cells (LSCs). CDK8, a kinase component of the Mediator complex, regulates oncogenic transcription, and the selective CDK8/CDK...

Are components of the histone gene expression machinery functionally repurposed in terminally differentiated cells?

The expression of metazoan replication-dependent histone genes is controlled by NPAT and U7 snRNP. NPAT activates transcription of histone genes during S-phase, whereas U7 snRNP is a multi-subunit endonuclease that cleaves the resultant transcripts at the 3' end, yielding mature...

High-field asymmetric waveform ion mobility spectrometry (FAIMS) improves proteome-wide N-homocysteinylation mapping in mouse liver and brain proteins

Abstract N -Homocysteinylation induces immunogenic, thrombogenic, and amyloidogenic properties of proteins. Although important to gain insight into the mechanisms of homocysteine (Hcy) toxicity, proteome-wide studies of the effects of Hcy-thiolactone (HTL) protein modification re...