Howard Hughes Medical Institute

Photometallobiocatalytic Asymmetric Radical‐Mediated Cross‐Coupling of Organotrifluoroborate Salts and Pyridotriazoles

ABSTRACT The cooperative integration of photoredox catalysis and metalloenzyme catalysis has emerged as a powerful strategy for enabling stereoselective radical transformations beyond the capabilities of either catalytic mode alone. Herein, we report a photometallobiocatalytic en...

Accelerating protein design by scaling experimental characterization

Abstract Recent advances in de novo protein design have greatly outpaced standard protein biochemistry workflows, and experimental testing has been a bottleneck in the validation of new designs and methodologies. Here, we describe experimental and computational workflows to addre...

Autoantibodies neutralizing type I interferons underlie a third of cases of Chikungunya virus encephalitis or myelitis

Abstract Chikungunya virus (CHIKV) infection is typically not life-threatening but may, in rare cases, affect the central nervous system (CNS). In three cohorts of confirmed CHIKV cases from Martinique (French overseas territory) and Brazil (patients aged 0–89 years, n = 245), 20...

The combination of morphogenic regulators BABY BOOM and GRF ‐ GIF improves maize transformation efficiency and promotes leaf regeneration

Summary Transformation is an indispensable tool for plant genetics and functional genomics. Although stable transformation in maize is no longer a major obstacle, there remains a need for accessible and efficient methods for academic laboratories. Here, we present the GGB system,...

Potent type-specific de novo antibodies complement broadly reactive imprinted antibodies in immune responses to SARS-CoV-2 variants

Abstract For rapidly mutating viruses such as influenza viruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), immune memory recalled by antigenically drifted variants primarily comprises antibodies that cross-react to the priming strain rather than de novo eli...

The Biomass Distribution on Earth

<p>This resource uses an interactive Voronoi diagram to explore global biomass and abundance data across major taxonomic groups, based on Bar-On, Phillips, and Milo (2018). It compares organisms across environments and measurement types, with guided activities for additiona...

RTF2 and CLASPIN localize CHK1 to the replication fork to control S-phase progression

The essential kinase ataxia telangiectasia and Rad3-related (ATR) monitors the replicative (S) phase of the cell cycle to ensure faithful propagation of genetic material. While much is known about the mechanisms governing ATR activity at replication forks, how its effector checkp...

A Workflow for the Identification of Oligomeric Structures on Tilted Sample Planes in Cryo-SMLM

We introduce a workflow to identify oligomeric structures that are recorded with single-molecule localization microscopy (SMLM) under cryogenic conditions. Typically, these oligomers are assumed to consist of protomers arranged as equilateral two-dimensional polygons and every pr...