Hangzhou Medical College

A non-invasive diagnostic model for early detection of gastric and colorectal cancers using serum glycopeptide profiling and machine learning

Gastrointestinal cancers are leading causes of cancer-related mortality worldwide, with early detection significantly improving survival outcomes. However, current diagnostic methods are either invasive or lack sufficient sensitivity for early-stage disease. This study aimed to d...

Single-Tube Six-Channel Ultrafast PCR with Melting-Curve Discrimination for Seven Respiratory Pathogens

Abstract Expanding single-tube multiplexing beyond the number of fluorescence channels on a real-time PCR instrument is a persistent challenge for point-of-care testing (POCT). Here, we break this barrier by leveraging melting temperature (Tm) as an extra information dimension. B...

Preoperative planning of venturi-like lymphaticovenous anastomosis for secondary lower extremity lymphedema using contrast-enhanced ultrasound

Venturi-like lymphaticovenous anastomosis (LVA), which utilizes venular branches as recipient veins, is hypothesized to be beneficial for maintaining postoperative anastomotic patency. This study assessed the impact of preoperative contrast-enhanced ultrasound lymphography (CEUS-...

Xanthatin directly targets ZDHHC7 to inhibit NLRP3 palmitoylation and inflammasome activation, alleviating sepsis and acute lung injury

Aberrant activation of the NLRP3 inflammasome drives the pathogenesis of diverse inflammatory diseases, including acute respiratory distress syndrome (ARDS) and sepsis. Among the post-translational modifications regulating NLRP3, S-palmitoylation contributes to NLRP3 localization...

MRC1⁺ macrophages and endothelial PTPRM signalling characterize a microvascular invasion–associated tumor ecosystem in hepatocellular carcinoma

The intercellular interactions and molecular features underlying microvascular invasion (MVI) in hepatocellular carcinoma (HCC) remain incompletely understood. We aimed to characterize the MVI-associated multicellular ecosystem at single-cell resolution and to derive prognostic a...

The bidirectional immunoregulatory effects of Traditional Chinese Medicine on the cGAS-STING signaling pathway and their translational prospects

The cyclic GMP-AMP synthase–stimulator of interferon genes (cGAS-STING) pathway is a central component of innate immunity, mediating host defense against pathogens, antitumor responses, and inflammation. Aberrant activation of this pathway is implicated in a spectrum of diseases,...

Electrokinetic Enrichment and Purification of Proteins and Anions from High-salt Samples on Liquid Plasticine-Based Open Chip

Abstract Microfluidic chips are promising for on-site analysis in medical diagnostics and food safety because they enable miniaturized, integrated sample processing. However, the analysis of complex, salt-rich matrices of biological and food samples remain challenging for convent...

FXYD1 participates in regulating sperm motility, capacitation, and acrosome reaction via associations with NKA/NCX1, ion homeostasis, membrane potential, and acrosin activity, with distinct localization in mouse and human sperm

Abstract Background FXYD1 plays a significant role in diverse biological processes and diseases. However, whether FXYD1 regulates sperm function is unknown. Methods We investigated the effects of FXYD1 on motility, capacitation, and the acrosome reaction, as well as the potential...

MFR, SVR and ASVR as promising biomarkers in overweight and obese population with obstructive sleep apnea syndrome: insights from an observational study

Background Obesity is an important risk factor of obstructive sleep apnea syndrome (OSAS), but not all obese people can develop OSAS. The role of novel human body composition indices—muscle-to-fat ratio (MFR), skeletal muscle mass-to-visceral fat area ratio (SVR) and appendicular...

Multivariate-Activated DNA Logic Gate for Predicting Responses to Cisplatin-Based Chemotherapy

Abstract Cisplatin-based chemotherapy remains an attractive alternative for controlling advanced cancers. However, a subset of cancer patients exhibits resistance to cisplatin. Accurate and early prediction of individual responses to cisplatin treatment is critical to personalize...

Cellular subpopulation remodeling and biphasic molecular dynamics in glioma recurrence: a multi-omics analysis and diagnostic markers screening using liquid biopsy samples

OBJECTIVE: Glioblastoma (GBM) is a highly aggressive brain tumor with a median survival of about 15 months and frequent recurrence. Liquid biopsy of cerebrospinal fluid (CSF) and plasma enables minimally invasive detection of tumor-associated molecular signals and may improve ear...

Core–Shell Nanofibrous Membranes for Dynamic Intestinal Organ-on-a-Chip Culture

Abstract Organ-on-a-chip (OOC) systems rely on membrane-supported tissue interfaces to replicate organ-level barrier functions in vitro. However, conventional porous membranes often provide limited biological cues for cell adhesion and may require an extracellular matrix coating....

Angelica keiskei extract inhibits LPS-induced activation of microglia and ameliorates acute cerebral ischemic injury in mice

Angelica keiskei (Miq.) Koidz. [Apiaceae] is a botanical drug with dual-purpose as food and medicine and its improvement in acute cerebral ischemic injury has not been studied. In the present study, we investigated the anti-neuroinflammatory and protective effects of the ethyl ac...

High immunosuppressants variability adversely affects post-transplant recurrence for patients with hepatocellular carcinoma

Tumor recurrence after liver transplantation (LT) for hepatocellular carcinoma (HCC) is the most significant obstacle impacting the prognosis of patients. Managing the clinical application of immunosuppressive agents is difficult, owing to their limited therapeutic range and nota...

Type I and II pili mediate transcytosis of Streptococcus pneumoniae across blood-brain barrier cells in the pathogenesis of pneumococcal meningitis

Streptococcus pneumoniae , a common pathogen to cause pneumonia and otitis media, is also the most important causative agent of bacterial meningitis in humans. Previous studies have shown that S. pneumoniae strains can be pilus-negative, type I pilus- and/or type II pilus-positiv...

Serum ferritin and ADHD in children: a case-control study accounting for inflammation

To evaluate the association between serum ferritin (SF) and attention-deficit/hyperactivity disorder (ADHD) in children while accounting for C-reactive protein (CRP). This retrospective case-control study included 156 children with DSM-5 ADHD and 164 healthy controls aged 6–12 ye...

CAR‐Engineered Cell Therapies Beyond Cancer: Reprogramming Fibrosis and Immune‐Mediated Inflammation

ABSTRACT Chimeric antigen receptor (CAR)‐engineered cell therapies are being extended from hematological malignancies to autoimmune, inflammatory, and fibrotic diseases, although the maturity of evidence differs markedly among platforms and indications. Early clinical evidence fr...

Iron‐Sulfur Cluster Targeting: A Novel Perspective for Treating Alcohol‐Related Lipid Metabolic Diseases

ABSTRACT Iron–sulfur (Fe–S) clusters are pivotal molecular cofactors bridging inorganic chemistry and organic life processes, with their tightly regulated biosynthesis and function underpinning cellular metabolic homeostasis. Chronic alcohol‐induced metabolic stress impairs Fe–S...

Polygonum cuspidatum Exosome‐Like Nanovesicles Alleviate Hypoxic Pulmonary Hypertension by Stabilizing PON1 to Inhibit MAPK ‐Mediated PASMC Phenotypic Switching

Hypoxic pulmonary hypertension (HPH) is characterized by excessive proliferation of pulmonary vascular cells, ultimately resulting in right heart failure and death. Although resveratrol, an active component of Polygonum cuspidatum (PC), has shown efficacy against HPH, the mechani...

Astaxanthin supplementation and cardiometabolic risk factors in overweight and obese adults: a GRADE-assessed systematic review and meta-analysis

Astaxanthin, a marine-derived carotenoid, has shown antioxidant and anti-inflammatory properties in studies. However, its effects on cardiometabolic risk factors in overweight and obese populations remain inconclusive. Therefore, this systematic review and meta-analysis aimed to...