General University Hospital in Prague

Pharmacokinetics and immunomodulatory effects of selective orexin 1 receptor agonist OXA 17–33: PBMC responses, HPA axis activation, and outcomes in the collagen-induced arthritis model

Given the expanding clinical use of orexin-modulating drugs, this study investigated whether orexin agonism or antagonism influences inflammatory responses in vitro and in vivo. PBMCs stimulated with LPS or TNF were treated with orexin agonists or antagonists. In rats, pharmacoki...

Grid to glide: A simple visual cue that alleviates upper-limb freezing

Freezing of the upper limb (FoUL) is a poorly explored manifestation of Parkinson's disease. This report describes a case of a 54-year-old woman with FoUL predominantly affecting handwriting. Writing on grid paper with small squares (0.5 × 0.5 cm) markedly reduced FoUL frequency....

Sacituzumab govitecan in heavily pretreated metastatic triple-negative breast cancer: a secondary analysis of the CEBCC-102 real-world cohort

Abstract Background Real-world evidence on sacituzumab govitecan (SG) in heavily pretreated patients with metastatic triple-negative breast cancer (mTNBC) remains limited. This study assessed SG outcomes in a multicentre Central European cohort. Methods This retrospective study i...

Prolonged low-dose intravenous thrombolysis in early recurrent stroke

INTRODUCTION: Treatment of acute ischemic stroke follows well-established guidelines and standards of care. However, the optimal management of ultra-early stroke recurrence remains unclear. We present outcomes of prolonged low-dose intravenous thrombolysis used as rescue therapy...

Methylene blue modulates mitochondrial electron flow via multiple regulatory sites in isolated brain mitochondria: Implications for neurodegeneration

Methylene blue (MB) is a redox-active compound capable of modulating the efficiency of the mitochondrial electron transport system (ETS). We investigated the acute dose-dependent effects of MB on oxygen consumption and H₂O₂ production in isolated brain mitochondria. High-resoluti...

Two-year smartphone motor monitoring in isolated REM sleep behavior disorder and Parkinson’s disease

Abstract We aimed to assess the sensitivity of key motor smartphone tasks to detect disease progression in subjects with isolated rapid eye movement sleep behavior disorder (iRBD) and early Parkinson’s disease (PD) compared to healthy controls. Twenty-six healthy controls, 22 pat...

Large-scale esophageal cancer screening through noncontrast computed tomography and artificial intelligence

The absence of accurate, noninvasive, scalable screening tools keeps early esophageal cancer (EC) detection a global health challenge. Although noncontrast computed tomography (NC CT) is widely accessible, the esophagus is a hollow tubular structure prone to collapse and motion a...

Delivering the EU Safe Hearts Plan: the essential role of laboratory medicine in cardiovascular health

Abstract The European Union Safe Hearts Plan provides an opportunity to strengthen cardiovascular prevention, early detection, treatment, and monitoring across Europe. This EFLM position paper argues that laboratory medicine must be recognized as a central component of its implem...

Nanopore long-read sequencing facilitates accurate diagnosis of KMT2B-related dystonia

Abstract Diagnosis of KMT2B -related dystonia remains challenging due to the high prevalence of variants of uncertain significance and technological constraint of short-read pipelines. To overcome these limitations, we integrated nanopore-based long-read sequencing with a validat...

Diagnostic pathways, healthcare utilization, and mortality in children and young people with early onset substance use disorders: a nationwide registry-based cohort study

Longitudinal, population-based evidence on the clinical profiles and mortality of youth with substance use disorders (SUDs) is limited, despite significant health and social risks. This study aimed to (1) characterize initial SUD diagnoses, demographic and healthcare profiles, an...

High-grade serous ovarian cancer is associated with increased TP53 mutation burden in uterine lavage

High grade serous ovarian cancer (HGSC) has low survival partly due to the lack of methods for detection, diagnosis, and risk prediction. TP53 mutations, which drive HGSC, are found in gynecological tissues as the result of somatic evolution, but it is unknown whether an excess o...

Giant Well-Differentiated Liposarcoma of the Spermatic Cord Associated With Inguinal Hernia: Clinical Insights From a Case Report

Patient: Male

The Impact of Robotization on Employees' Mental and Physical Health: A Qualitative Study in the Czech and Slovak Republics

BACKGROUND: This study aimed to explore the impact of introducing robotic technologies on employees' mental and physical health across three Czech and one Slovak manufacturing company. It sought to understand employees' subjective experiences and perceptions of organizational sup...