First Affiliated Hospital of Zhengzhou University

Nicotinamide Metabolism Constrains Memory CD8 + T Cell Formation Through a Putative HS1BP3‐SIRT1‐FOXO3‐BCL6 Axis

ABSTRACT Memory T cells exhibit long‐term persistence, a defining feature that underpins durable clinical responses to adoptive immunotherapies. The mechanisms that integrate metabolic cues with transcriptional control of memory fate remain undetermined. Here, we identify HS1‐bin...

A mixed-methods study on the effectiveness and optimization needs of a prescription review training program: from the KAP perspective of returning trainees

Abstract Background Prescription review training programs are widely implemented in China to meet increasingly stringent policy requirements for rational drug use. However, evidence on the effectiveness of these programs and the specific needs for their optimization, particularly...

AI-driven drug design for head and neck squamous cell carcinoma using GNN and structure-based simulations

Head and neck squamous cell carcinoma (HNSCC) remains a major global health burden with limited targeted therapeutic options. Here we present an integrated artificial intelligence (AI)-driven drug discovery framework combining graph neural networks (GAT, GCN, AttentiveFP and GIN)...

Biomolecular condensates as dynamic regulators of musculoskeletal homeostasis, disease, and therapeutic challenges

Abstract Biomolecular condensates are membraneless assemblies that concentrate proteins, nucleic acids, and other biomolecules into dynamic cellular compartments. Liquid-liquid phase separation (LLPS) is one important route by which such condensates form, particularly when multiv...

Efficacy and safety of urokinase intravenous thrombolysis in minor stroke according to large vessel occlusion status: a subgroup analysis of the TRUST trial

The risk-benefit profile of thrombolytic therapy in minor stroke patients with large vessel occlusion (LVO) remains uncertain. This post hoc analysis of the TRUST trial (ThRombolysis with Urokinase for minor Stroke Trial) sought to comparatively assess whether the presence of lar...

The SF3b complex in cancer: structural basis, molecular mechanisms, and therapeutic opportunities

Aberrant alternative splicing is increasingly recognized as a fundamental driver of cancer initiation and progression. The splicing factor 3b (SF3b) complex, an essential component of the U2 small nuclear ribonucleoprotein (snRNP), plays a pivotal role in branch point sequence (B...

Mefatinib versus gefitinib as a first-line treatment for EGFR-mutated non-small cell lung cancer: a randomized, double-blind, multicenter phase III study

Mefatinib, a novel second-generation epidermal growth factor (EGFR) tyrosine kinase inhibitor that has shown promising antitumor activity in targeting non-small cell lung cancer (NSCLC) with common and uncommon EGFR- activating mutations. In this phase III, randomized, double-bli...

Integrative multi-omics analysis identifies SMIM24 as a favorable prognostic biomarker in clear cell renal cell carcinoma

Clear cell renal cell carcinoma (ccRCC) exhibits substantial molecular and clinical heterogeneity, contributing to variable disease progression and therapeutic outcomes. We previously identified a de-clear cell differentiation (DCCD) tumor state characterized by loss of canonical...

Clinical utility of molecular residual disease detection in early-stage resected EGFR-mutated non-small cell lung cancer

Molecular residual disease (MRD), detected through circulating tumor DNA, has emerged as a promising biomarker for surveillance in patients with early-stage non-small cell lung cancer (NSCLC) following curative-intent resection. Here we report the MRD analysis from the phase 3 EV...

Noninvasive Detection of MASH and Fibrotic Burden Using Ultrasound‐Derived LIID and LSM in MASLD: A Multicenter Study

ABSTRACT Background and Aims Metabolic dysfunction‐associated steatohepatitis (MASH), the progressive form of metabolic dysfunction‐associated steatotic liver disease (MASLD), is associated with adverse hepatic and cardiometabolic outcomes. The study aimed to validate a novel qua...

Pan-modification proteomics: decoding tumor heterogeneity and guiding the next generation of precision oncology

Tumor heterogeneity is driven by genomic and transcriptomic variation, as well as by extensive protein diversification through post-translational modifications (PTMs), which generate distinct functional proteoforms. These combinatorial modifications reshape signaling, metabolism,...

Treatment of Acute Ischemic Stroke with Intravenous Urokinase (TASK-UK): Protocol for a Multicenter, Prospective, Real-World Study

Intravenous thrombolysis with recombinant tissue plasminogen activator (rt-PA) and urokinase (UK) are both recommended for acute ischemic stroke (AIS) in China. Compared with rt-PA, UK is less expensive and more widely available in clinical practice. The Treatment of Acute Ischem...

CT-guided biopsy of pulmonary ground-glass nodules: core-needle biopsy versus fine-needle aspiration

To compare the diagnostic performance and complication profiles of CT-guided core-needle biopsy (CNB) and fine-needle aspiration (FNA) for pulmonary ground-glass nodules (GGNs). Consecutive patients who underwent CT-guided percutaneous biopsy of pulmonary GGNs between January 202...

Synchronous ReactiveOxygen Species Scavenging andImmunomodulation for Autoimmune Uveitis Therapy by a MannosylatedBiomimetic Nanocloak

Abstract Autoimmune uveitis is a recurrent, vision-threatening inflammatory disease that demands precise and ongoing treatment. However, current therapies have limitations in ocular targeting, in controlling reactive oxygen species (ROS), and in regulating key inflammatory pathwa...

Combined administration of fructooligosaccharides and Clostridium butyricum attenuates bowel cleansing-induced dysbiosis and safeguards mucosal defense in a murine model

Bowel preparation with polyethylene glycol electrolyte solution (PEG-ELS) is indispensable for gastrointestinal endoscopy and related procedures. However, it disrupts gut microbial homeostasis and increases host vulnerability to ensuing infection. In a murine model, we optimized...