Azienda Ospedaliero-Universitaria Careggi

Rethinking Solid-Phase Microextraction (SPME) for Bioanalysis: Workflow Optimisation in Automated Vacuum-Assisted Cooling SPME for Fatty Acid Monitoring in Faecal Waters

Short-chain fatty acids (SCFAs) are key biomarkers of gut microbiota and intestinal health, yet their analysis is challenging due to high volatility, hydrophilicity, and the complexity of the faecal matrix. This study introduces a novel, green, and fully automated workflow for mo...

Evaluating Long-Term Effectiveness and Safety of Glaucoma Surgical Innovations: A Systematic Review

Précis: A systematic review examining reporting of long-term safety of glaucoma surgical innovations. 27 studies met inclusion criteria and demonstrated effectiveness over the short to medium term. Use of registries to optimise future long-term evaluation is recommended. Purpose:...

Extracorporeal Photopheresis as an Immunomodulatory Bridge in Kidney Transplant Recipients With Infections and Concomitant Donor‐Specific Antibodies: A Case Series

ABSTRACT Introduction Kidney transplant recipients with severe infections often require immunosuppression minimization, increasing the risk of donor‐specific antibody (DSA) persistence and antibody‐mediated injury. Extracorporeal photopheresis (ECP) may provide immunomodulation w...

Preliminary evidence of long‐term safety and effectiveness of anti‐ CGRP drugs for migraine prevention in people with comorbid epilepsy: A 52‐week follow‐up retrospective study

Abstract Migraine and epilepsy frequently coexist, but evidence on the safety of calcitonin gene‐related peptide (CGRP)‐targeting therapies in people with epilepsy is lacking. We evaluated long‐term migraine and epilepsy outcomes in a real‐world multicenter retrospective cohort o...

Travel distance to tertiary healthcare reflects disease severity and medication access in Italian systemic sclerosis patients: a geographical study on adult-onset rare diseases from SPRING-SIR registry

Systemic sclerosis (SSc) is a rare, multiorgan disease in adults that requires specialized care. Commuting to tertiary medical centers poses a considerable burden and incurs significant costs, potentially affecting management choices. This study aims to describe the healthcare co...

Autologous haematopoietic stem cell transplantation affects paramagnetic rim lesions in multiple sclerosis

Background: Available literature shows a marginal or no effect of disease-modifying therapies on paramagnetic rim lesions (PRLs), a promising magnetic resonance imaging marker of chronic inflammation in multiple sclerosis. Objective: To investigate longitudinal changes in PRL cou...

Validation of the International Working Group 2023 response criteria for higher risk myelodysplastic syndromes/neoplasms using the International Consortium for Myelodysplastic Syndromes VALIDATE database and a randomized phase III trial

Response assessment criteria in patients with higher-risk myelodysplastic syndromes/neoplasms (HR-MDS) have been updated in the 2023 International Working Group (IWG) criteria. However, validation of these criteria has been limited to date. We analyzed overall survival (OS) by be...

Doxapram in Preterm Infants: From Respiratory Stimulation to Individualized Therapy—A Narrative Review

Background: Apnea of prematurity (AOP) remains one of the most common complications of preterm birth, and despite the established role of caffeine as first-line therapy, a proportion of extremely preterm infants continue to experience clinically significant apnea. Doxapram, a res...

Postpandemic Trends in Pediatric Respiratory Virus Hospitalizations: A Multicenter Study, 2019–2025

ABSTRACT Background The COVID‐19 pandemic profoundly altered the circulation of respiratory viruses in children, changing the epidemiology of acute lower respiratory tract infections. In the postpandemic period, respiratory pathogens showed marked temporal variability. The 2024–2...

CALR ‐mutated myeloproliferative neoplasms: Refining molecular risk beyond the driver

Calreticulin ( CALR )‐mutated myeloproliferative neoplasms (MPNs) are generally associated with favourable outcomes, yet considerable heterogeneity persists across the disease course. Mawalkar et al. provide long‐term data across essential thrombocythaemia (ET), post‐ET myelofibr...

Long‐Term Progression to Permanent Atrial Fibrillation After Cryoballoon Pulmonary Vein Isolation: Insights From the Multicenter 1STOP Project

ABSTRACT Background Atrial fibrillation (AF) often progresses from paroxysmal to persistent forms, culminating in permanent AF, a transition associated with increased risks of stroke, heart failure, and hospitalization. Cryoballoon pulmonary vein isolation (CB‐PVI) is an establis...

Real-world adjunctive use of an alpha-lactalbumin/sodium butyrate Food for Special Medical Purposes in drug-resistant epilepsy: the multicentre retrospective ARMILLA study

The purpose of this study was to describe real-world adjunctive use, tolerability, and hypothesis-generating seizure and multidomain outcomes of LALBAAY, an alpha-lactalbumin/sodium butyrate Food for Special Medical Purposes (FSMP), in patients with drug-resistant epilepsy (DRE)....

Beyond BMI : Unsupervised Machine Learning Clustering of Bioelectrical Impedance Phenotypes Is Associated With Weight‐Recovery Trajectories in Anorexia Nervosa

ABSTRACT Objective To identify data‐driven body composition phenotypes in patients with moderate‐to‐extreme anorexia nervosa (AN) using machine learning (ML) clustering of bioelectrical impedance analysis (BIA) parameters, and to test whether these phenotypes have specific psycho...

Effect Modification of Rescue Stenting in ICAS-Related Middle Cerebral Artery Occlusion: The Role of Thrombectomy Failure, Stenosis Severity, and ASPECTS

Rescue stenting (RS) is increasingly used for intracranial artery stenosis-related large vessel occlusion (ICAS-LVO), but optimal patient selection remains uncertain. We investigated the baseline and procedural factors associated with receiving RS and whether they modified the as...

When should clinical practice guidelines change? A critical appraisal of the ESVS focused update on paclitaxel-coated devices after SWEDEPAD 2

Clinical practice guidelines represent the most rigorous level of evidence synthesis available to clinicians. Their authority stems not only from the quality of individual studies, but also from the thoroughness with which the entire body of evidence is critically appraised, bala...

Prognostic Role of Adverse Events in a Worldwide Population of Patients with Biliary Tract Cancer Treated with Chemo-Immunotherapy

Background: Biliary tract cancers (BTCs) are aggressive malignancies with limited treatment options. The addition of durvalumab or pembrolizumab to cisplatin–gemcitabine improves overall survival (OS), but validated biomarkers of benefit remain limited. Immune-mediated adverse ev...

A distinct gastrointestinal symptom profile characterizes clinically relevant fatigue in systemic sclerosis

OBJECTIVE: To identify the specific gastrointestinal (GI) symptoms associated with clinically relevant fatigue in systemic sclerosis (SSc), beyond the established association between overall GI involvement and fatigue. METHODS: We conducted a cross-sectional study including conse...

Early Versus Late Biliary Leakage After Hepatic Resection: Incidence, Risk Profiles, and Clinical Impact of Diagnostic Timing

Background/Objectives: Post-hepatectomy biliary leakage (BL) remains a major source of postoperative morbidity. While traditional risk models treat BL as a single clinical entity, its chronological presentation follows distinct patterns that raise the hypothesis of distinct under...

Combined Botulinum Toxin Type A and Hyaluronic Acid Versus Exclusive Hyaluronic Acid Injections: A Potential Nonsurgical Alternative for Chin Augmentation

Nonsurgical chin augmentation with hyaluronic acid (HA) fillers is a widely used technique in aesthetic medicine. However, outcomes may be influenced by hyperactivity of the mentalis muscle, which may lead to reduced longevity. Botulinum toxin type A (BoNT-A) may improve filler i...

International Society of Urological Pathology Multidisciplinary Expert Consultation Conference on Evaluation of Treatment Effects in Prostate and Bladder Cancer

Treatment effects in the urinary bladder are variable and range from localized post-transurethral resection changes to widespread systemic therapy effects. The International Society of Urological Pathology (ISUP) organized a consensus meeting in Vienna, Austria, in September 2025...