Tomohisa Hasunuma

The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance

Abstract Rubisco is an important but challenging protein engineering target. Fast and selective rubiscos could enhance photosynthesis in plants and accelerate biobased production processes. To facilitate engineering of rubisco, we applied an in vivo screen that couples rubisco ac...

Engineering a Highly meta -Selective Biphenyl Monooxygenase for the Biosynthesis of High-Value Polymer Precursors

Abstract The meta-selective C–H functionalization of aromatic substrates lacking meta-directing electron-withdrawing groups remains challenging. To engineer the regioselective enzymatic conversion of biphenyl (BP) to 3,3′-dihydroxybiphenyl (33DHBP), an industrial precursor to hig...