Peter L. Oliver

A 4′‐Thioribouridine Modification Mimics 2′‐ O ‐Methoxyethyl in ASO‐Like 20‐Mers Targeting PTEN Knockdown

Gapmer antisense oligonucleotides (ASOs) represent a clinically validated therapeutic platform, with a 2′‐ O ‐methoxyethyl modification commonplace within the flanking regions. An alternative sugar modification, incorporation of 4′‐thioribose, has demonstrated enhanced nuclease r...

Accurately modeling RNase H-mediated antisense oligonucleotide efficacy.

, achieving a 5.72-fold reduction in screening effort compared to random selection. ASO Atlas provides the first systematic resource to rigorously evaluate hypotheses regarding key parameters in ASO design, including sequence composition, chemical modifications, and target region...

Cortical layer 6b mediates state-dependent changes in brain activity and effects of orexin on waking and sleep.

One of the most distinctive features of the mammalian cerebral cortex is its laminar structure. Of all cortical layers, layer 6b (L6b) is by far the least studied, despite exhibiting direct sensitivity to orexin and having widespread connectivity, suggesting an important role in...