David A. Baker

A scalable recombinant pipeline for disulphide-stapled peptides — code and data

Analysis code and sequence-free source data for a 96-well recombinant pipeline for disulphide-stapled cyclic peptides: automated IVA primer design, qPCR cloning QC, periplasmic secretion and DOC lysis, IMAC, SEC yield analysis with hierarchical clustering, and Ellman's free-thiol...

De Novo Design and Structural Optimization of Mn(salen)‐Based Artificial Metalloenzymes for Asymmetric Sulfoxidation

ABSTRACT Artificial metalloenzymes (ArMs) exhibit exceptional selectivity, yet extending their reactivity beyond native cofactors remains a major challenge. While previous designs using native protein scaffolds to incorporate nonnative cofactors have been reported, de novo protei...

Bifunctional Phagocytic Synapse Enhancers Remodel the Tumor Microenvironment to Overcome Immunosuppression

Abstract Current immunotherapies often fail in immunologically “cold”, macrophage-rich tumor microenvironments (TMEs). Multi-targeting approaches that modulate innate-adaptive immune activation represent a promising frontier in cancer immunotherapy. Here, we developed phagocytic...

Accelerating protein design by scaling experimental characterization

Abstract Recent advances in de novo protein design have greatly outpaced standard protein biochemistry workflows, and experimental testing has been a bottleneck in the validation of new designs and methodologies. Here, we describe experimental and computational workflows to addre...