STAR Protocols question-and-answer series: Flow cytometry

Abstract

Flow cytometry is an integral part of the research scientist's toolbox. However, it is a complex methodology, involving decisions from antibody panel design to gating strategies to obtain the best results. Because it is so commonly used, there are many frequently asked questions about the protocols underlying its use. In this Backstory, STAR Protocols authors, reviewers, and advisory board members share their knowledge and expertise regarding common technical questions about flow cytometry.